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| Status |
Public on Aug 01, 2018 |
| Title |
END-seq profile in B,T, and HCT116 untreated and upon HU treatment |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
|
| Summary |
In this study, we map sites of replication initiation and breakage in primary cells at high resolution under conditions of replication stress. We show that replication initiates between transcribed genes within nucleosome-depleted structures established by long asymmetrical poly(dA:dT) tracts flanking the initiation site. Paradoxically, large (>20 bp) homopolymeric (dA/dT) tracts are also preferential sites of polar replication fork stalling and collapse. We propose that the evolutionary expansion of poly(dA:dT) tracts in eukaryotic genomes serves to promote replication initiation, but at the cost of increasing chromosome fragility.
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| Overall design |
END-seq profile in B,T, and HCT116 untreated and upon HU treatment.
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| Contributor(s) |
Tubbs A, Sridharan S, van Wietmarschen N, Maman Y, Callen E, Stanlie A, Wu W, Wu X, Day A, Wong N, Yin M, Canela A, Fu H, Redon C, Pruitt S, Jaszczyszyn Y, Aladjem M, Aplan P, Hyrien O, Nussenzweig A |
| Citation(s) |
30078706 |
| |
| Submission date |
Jun 27, 2018 |
| Last update date |
Jul 02, 2019 |
| Contact name |
Andre Nussenzweig |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institutes of Health
|
| Department |
National Cancer Institute
|
| Lab |
Laboratory of Genome Integrity
|
| Street address |
9000 Rockville Pike, Building 37
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (2) |
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| Samples (14)
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| GSM3227947 |
ES4: END_seq_aB_28h_NT_rep1 |
| GSM3227948 |
ES5: END_seq_aB_28h_NT_rep2 |
| GSM3227949 |
ES6: END_seq_aB_28h_HU_28h_0.5mM_rep1 |
| GSM3227950 |
ES7: END_seq_aT_28h_HU_28h_2mM_rep1 |
| GSM3227951 |
ES8: END_seq_aHCT116_8h_HU_8h_10mM_rep1 |
| GSM3227952 |
ES9: END_seq_aHCT116_8h_NT_rep1 |
| GSM3227953 |
ES10: END_seq_aB_28h_CDC2i_CDC7i_0.5h_HU_6h_10mM_rep1 |
| GSM3227954 |
ES11: END_seq_aB_28h_CDC2i_CDC7i_0.5h_HU_6h_0.5mM_rep1 |
| GSM3227955 |
ES12: END_seq_aB_28h_ATRi_28h_rep1 |
| GSM3227956 |
ES13: END_seq_aB_28h_HU_28h_0.5mM_BL6_eij_rep1 |
| GSM3227957 |
ES14: END_seq_aB_28h_HU_28h_0.5mM_CAST_eij_rep1 |
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| This SubSeries is part of SuperSeries: |
| GSE116321 |
Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork Collapse |
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| Relations |
| BioProject |
PRJNA478132 |
| SRA |
SRP151419 |
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