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| Status |
Public on Jun 18, 2019 |
| Title |
The MybMuvB complex is required for YAP dependent transcription of mitotic genes |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
YAP/TAZ, downstream effectors of the Hippo pathway, are important regulators of proliferation. Here we show that the ability of YAP to activate mitotic gene expression is dependent on the Myb-MuvB (MMB) complex, a master regulator of genes expressed in the G2/M phase of the cell cycle. By carrying out genome-wide expression and binding analyses, we found that YAP promotes binding of the MMB subunit B-MYB to the promoters of mitotic target genes. YAP binds to B-MYB and stimulates B-MYB chromatin-association through distal enhancer elements that interact with MMB-regulated promoters through chromatin looping. The cooperation between YAP and B-MYB is critical for YAP-mediated entry into mitosis. Furthermore, the expression of genes co-activated by YAP and B-MYB is associated with poor survival of cancer patients. Together, our findings provide a molecular mechanism by which YAP and MMB regulate mitotic gene expression and suggest a link between two cancer-relevant signaling pathways.
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| |
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| Overall design |
ChIP-seq of LIN9, YAP, B-MYB and histone marks in murine lung adenocarcinoma cells (KPL) and in human MCF10A-YAP5SA cells.
Gene expression changes upon Lin9-depletion in KPL cells were measured with RNA-seq.
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| Contributor(s) |
Pattschull G, Walz S, Ruehl E, Wolf E, Ade CP, Kneitz S, Rosenwald A, von Eyss B, Gaubatz S |
| Citation(s) |
31216474 |
| |
| Submission date |
Jun 13, 2018 |
| Last update date |
Jun 27, 2019 |
| Contact name |
Stefan Gaubatz |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Wuerzburg
|
| Department |
Physiological Chemistry
|
| Lab |
Gaubatz
|
| Street address |
Am Hubland
|
| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (33)
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| Relations |
| BioProject |
PRJNA476018 |
| SRA |
SRP150501 |
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