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| Status |
Public on Jan 21, 2009 |
| Title |
The Arabidopsis BRAHMA Chromatin Remodelling ATPase Is Involved in Direct Repression of Embryonic Traits in Leaves |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Synthesis and accumulation of seed storage proteins (SSPs) is an important aspect of the seed maturation program. Genes encoding SSPs are specifically and highly expressed in the seed during maturation. However, the mechanisms that repress the expression of these genes in leaf tissue are not well understood. To gain insight into the repression mechanisms, we have performed a transgenic screening for mutants that express SSPs in leaves. Here we show that mutations of BRAHMA (BRM), a SNF2 chromatin remodelling ATPase, cause the ectopic expression of a subset of SSPs and other embryogenesis related genes in leaf tissue. Consistent with the notion that such SNF2-like ATPases form protein complexes in vivo, we observed similar phenotypes for mutations of AtSWI3C, a BRM interacting partner, and BSH, a SNF5 homolog and essential SWI/SNF subunit. Further, we present chromatin immunoprecipitation evidence that BRM is recruited to the promoters of a number of embryogenesis genes including the 2S genes, which are expressed/elevated in brm leaves. Consistent with its role in nucleosome remodelling, BRM appears to control the chromatin structure of the At2S2 promoter. These results show that a BRM-containing chromatin remodelling ATPase complex is involved in the direct repression of SSPs in leaf tissue.
A matrix comprising the signal intensity value of each gene per replicate hybridization and the averaged data of each gene generated from three replicate hybridizations of the wild type and mutant samples, respectively, is linked below as a supplementary file.
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| Overall design |
Total RNA was isolated from three independent biological replicates of essp3 mutant and Wild type (ProβCG:GUS) respectively. Three ATH1 chips were used for the mutant and three for the wild type.
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| Contributor(s) |
Tang X, Hou A, Babu M, Nguyen V, Hurtado L, Lu Q, Reyes J, Wang A, Keller W, Tsang E, Cui Y |
| Citation(s) |
18508955 |
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| Submission date |
May 19, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mohan babu |
| E-mail(s) |
[email protected]
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| Phone |
5194571470
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| Fax |
5194573997
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| Organization name |
AAFC
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| Department |
Virology
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| Lab |
Molecular Virology
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| Street address |
1391 Sandford
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| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N5V 4T3 |
| Country |
Canada |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM289637 |
essp3/brm5 mutant sample_biological replicate 1 |
| GSM289638 |
wild type (ProβCG:GUS) sample_biological replicate 1 |
| GSM289639 |
essp3/brm5 mutant sample_biological replicate 2 |
| GSM289640 |
essp3/brm5 mutant sample_biological replicate 3 |
| GSM289641 |
wild type (ProβCG:GUS) sample_biological replicate 3 |
| GSM289642 |
wild type (ProβCG:GUS) sample_biological replicate 2 |
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| Relations |
| BioProject |
PRJNA106329 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11505_ESSP3_and_WT.txt |
5.0 Mb |
(ftp)(http) |
TXT |
| GSE11505_RAW.tar |
19.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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