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| Status |
Public on Oct 18, 2019 |
| Title |
Absolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
High-level H3K27me3 domains are required for proper gene repression mediated by Polycomb proteins. Genome-wide profiling of H3K27 methylation during developmental progression and in animals with reduced PRC2 levels illustrates how mass spectrometry-based normalization permits the quantitative analysis of changes in chromatin states.
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| |
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| Overall design |
Definition of high-level H3K27me3 domains by genome segmentation, direct comparison of H3K27me3 profiles in 2-4 hr and 14-16 hr wt embryos as well as in 0-6 hr wt and esc mutant embryos.
|
| Web link |
https://www.cell.com/developmental-cell/fulltext/S1534-5807(19)30767-1
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| |
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| Contributor(s) |
Müller J, Bonnet J, Gagneur J, Stricker G, Çelik MH |
| Citation(s) |
31630981 |
| |
| Submission date |
May 23, 2018 |
| Last update date |
Jan 20, 2020 |
| Contact name |
Jürg Müller |
| Organization name |
Max Planck Institute of Biochemistry
|
| Street address |
Am Klopferspitz
|
| City |
Martinsried |
| State/province |
Bavaria |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
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| Platforms (2) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA472784 |
| SRA |
SRP148798 |
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