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| Status |
Public on Nov 02, 2022 |
| Title |
Next generation sequencing to determine changes in Drosophila Atg6 mutant larval transcriptome |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Next generation sequencing was performed on third instar Drosophila larvae to determine changes in the transcriptomes of Atg6 heterozygous and Atg6 homozygous animals compared to wild type animals
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| Overall design |
RNA was isolated from Drosophila third instar larvae of the genotypes w118, w118;;Atg6-/+, and w118;;Atg6-/- using Qiagen Rneasy kit. Paired-end next generation RNA sequencing (20 milliion reads at 2 x 75bp PE) was performed in triplicate for each sample using illumina
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| |
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| Contributor(s) |
Doherty J |
| Citation(s) |
35523956 |
| |
| Submission date |
May 22, 2018 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Julie Zhu |
| E-mail(s) |
[email protected]
|
| Phone |
5088565377
|
| Organization name |
UMass Medical School
|
| Department |
Molecular Cellular and Cancer Biology
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA472626 |
| SRA |
SRP148761 |
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