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| Status |
Public on Sep 01, 2018 |
| Title |
Single cell RNA-sequencing of visceral adipose tissue Pdgfrβ+ cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously derived a doxycycline-inducible (Tet-On) lineage-tracing model that allows for the indelible labeling of Pdgfrb-expressing perivascular cells in adipose tissue of adult mice (PdgfrbrtTA; TRE-Cre; Rosa26RmT/mG; herein, “MuralChaser mice”). Prior to exposing animals to doxycyline, all cells within the stromal-vascular fraction (SVF) of adult gonadal WAT (gWAT) express membrane tdTomato from the Rosa26 locus. Following 9 days of exposure to doxycycline-containing chow diet, Cre-mediated excision of the loxP-flanked tdTomato cassette occurs in Pdgfrb-expressing cells, and membrane-bound GFP (mGFP) expression is constitutively activated. We set out to test the hypothesis that Pdgfrb-expressing perivascular cells in gonadal visceral WAT of adult mice are heterogeneous, with subpopulations harboring functionally distinct phenotypes. To this end, we performed single cell RNA-sequencing of mGFP+ cells isolated from gWAT of lean (chow fed) 8 weeks-old male MuralChaser mice following 9 days of doxycycline exposure.
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| Overall design |
We pooled tdTomato- GFP+ cells from gonadal white adipose tissue (WAT) from 5 MuralChaser mice. Single cell library preparation was performed using the 10X Genomics Single Cell 3’ v2 according to the manufacturer’s instructions.
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| Contributor(s) |
Gupta RK, Hepler C |
| Citation(s) |
30265241 |
| |
| Submission date |
Mar 08, 2018 |
| Last update date |
Jan 27, 2020 |
| Contact name |
Rana K Gupta |
| E-mail(s) |
[email protected]
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| Organization name |
Duke University
|
| Department |
Duke Molecular Physiology Institute
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| Street address |
Carmichael Building, 300 North Duke St.
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| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27701 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (1) |
| GSM3034641 |
Gonadal tdTomato- GFP+ cells from gonadal WAT |
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| This SubSeries is part of SuperSeries: |
| GSE144299 |
Single cell RNA-sequencing of gonadal and inguinal adipose tissue Pdgfrβ+ cells |
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| Relations |
| BioProject |
PRJNA437472 |
| SRA |
SRP134197 |
