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| Status |
Public on Feb 13, 2019 |
| Title |
RNA sequencing reveals RNA binding protein HuR/ELAVL1 regulates extracellular matrix gene expression in nucleus pulposus cells of the intervertebral disc |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used RNA sequencing to identify transcripts that were controlled by the RNA binding factor HuR/ELAVL1 in nucleus pulposus cells of the intervertebral disc.
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| Overall design |
Nucleus pulposus cells were isolated from rat intervertebral discs and cultured under hypoxic conditions. Cells were transduced with either control (MISSION PLKO.1 non-target ShRNA) or HuR-specific shRNA (ShHuR). mRNA was isolated using Rneasy mini columns (Qiagen), treated with DNase I, and RNA sequencing was performed. n=4 for each experimental group.
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| Contributor(s) |
Risbud MV, Pan H, Johnson ZI |
| Citation(s) |
30092282 |
| |
| Submission date |
Feb 19, 2018 |
| Last update date |
Feb 13, 2019 |
| Contact name |
Makarand Risbud |
| E-mail(s) |
[email protected]
|
| Phone |
(215) 955-1063
|
| Organization name |
Thomas Jefferson University
|
| Department |
Orthopaedic Surgery
|
| Lab |
511 College Bldg.
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| Street address |
1015 Walnut St.
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA434587 |
| SRA |
SRP133075 |
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