 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 30, 2018 |
| Title |
ChIP-seq profiling of transcriptional co-activator p300 during early myogenic differentiation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Molecular regulation of stem cell differentiation is exerted through both genetic and epigenetic determinants over distal regulatory or enhancer regions. Understanding the mechanistic action of active or poised enhancers is thus imperative for control of stem cell differentiation. Based on a genome-wide co-occurrence of different epigenetic marks in committed proliferating myoblasts, we have previously generated a 14-state chromatin state model to profile residue-specific histone acetylation in early myoblast differentiation. Here, we use genome-wide chromatin state association to delineate the functional mode of transcription regulators in early myogenic differentiation. We define a role of transcriptional coactivator p300, when recruited by master muscle regulator MyoD, in the establishment and regulation of myogenic loci at the onset of terminal differentiation. In addition, we reveal an enrichment of residue- and loci-specific histone acetylation at p300 associated active or poised enhancers, particularly when enlisted by MyoD. We provide novel molecular insights into the regulation of myogenic enhancers by p300 in concert with MyoD. Our studies present a valuable aptitude for driving stage specific chromatin state or enhancers pharmacologically to treat muscle related diseases and for the identification of additional myogenic targets and molecular interactions for therapeutic development.
|
| |
|
| Overall design |
We examined the genome-wide enrichment of transcriptional co-activator p300 in proliferating C2C12 myoblasts and myoblasts differentiated for 24 hours. Input chromatin DNA was used as control.
|
| |
|
| Contributor(s) |
Hamed M, Khilji S, Li Q |
| Citation(s) |
29927685, 36514349 |
| |
| Submission date |
Jan 25, 2018 |
| Last update date |
Jan 05, 2023 |
| Contact name |
Qiao Li |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Ottawa
|
| Department |
Cellular and Molecular Medicine
|
| Street address |
451 smyth road
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1H 8M5 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA431571 |
| SRA |
SRP131228 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109636_RAW.tar |
1.1 Gb |
(http)(custom) |
TAR (of BIGWIG, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
