|
| Status |
Public on Mar 29, 2008 |
| Title |
Gene expression in colon cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genetic and epigenetic defects in Wnt/ß-catenin signaling play important roles in colorectal cancer progression. Here we identify DACT3, a member of the DACT (Dpr/Frodo) gene family, as a negative regulator of Wnt/ß-catenin signaling that is transcriptionally repressed in colorectal cancer. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications. Remarkably, DACT3 expression can be robustly de-repressed by a pharmacological combination that simultaneously targets both histone methylation and deacetylation, leading to strong inhibition of Dishevelled (Dvl)-mediated Wnt/ß-catenin signaling and massive apoptosis of colorectal cancer cells. Our study identifies DACT3 as an important regulator of Wnt/ß-catenin signaling in colorectal cancer and suggests a potential strategy for therapeutic control of Wnt/ß-catenin signaling in colorectal cancer.
Keywords: Colon cancer cell line
|
| |
|
| Overall design |
Gene expression profile in HT29, DLD1 and SW480
|
| |
|
| Contributor(s) |
Yu Q |
| Citation(s) |
18538736 |
| |
| Submission date |
Mar 26, 2008 |
| Last update date |
Jan 18, 2013 |
| Contact name |
qiang yu |
| E-mail(s) |
[email protected]
|
| Organization name |
GIS
|
| Street address |
BIOPOLIS STREET
|
| City |
SINGAPORE |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL6104 |
Illumina humanRef-8 v2.0 expression beadchip |
|
| Samples (12)
|
| GSM277543 |
HT 29 Control (1688628068_A) |
| GSM277544 |
HT 29 Trichostatin A 200nM (1688628068_B) |
| GSM277545 |
HT 29 3-Deazaneplanocin 5µM (1688628068_E) |
| GSM277546 |
HT 29 3-Deazaneplanocin + Trichostatin A (1688628068_F) |
| GSM277547 |
DLD1 Control (1855529025_A) |
| GSM277548 |
DLD1 3-Deazaneplanocin 5µM (1855529025_B) |
| GSM277549 |
DLD1 Trichostatin A 200nM (1855529025_C) |
| GSM277550 |
DLD1 3-Deazaneplanocin + Trichostatin A (1855529025_D) |
| GSM277551 |
SW480 Control (1855529041_A) |
| GSM277552 |
SW480 3-Deazaneplanocin 5µM (1855529041_B) |
| GSM277553 |
SW480 Trichostatin A 100nM (1855529041_C) |
| GSM277554 |
SW480 3-Deazaneplanocin + Trichostatin A (1855529041_D) |
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA108791 |
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