|
| Status |
Public on Dec 21, 2017 |
| Title |
IKZF2 is required for myeloid leukemic stem cells by driving self-renewal and inhibiting the myeloid differentiation program I |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report that IKZF2 is required for maintaining chromatin accessibility in leukemic stem cells in myeloid leukemia. RNA seq and ATAC-seq were performed to investigate the changes in chromatin accessibility of differentially expressed genes in leukemic stem cells when IKZF2 was absent. We found that IKZF2 maintains open accessibility in self-renewal transcription factor motifs such as HOXA9 sites whereas motifs of differentiation transcription factors including C/EBPs are kept closed.
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| |
|
| Overall design |
4 RNA-seq, 4 ATAC-seq; 2 Ikzf2 WT each, 2 Ikzf2 KO each
|
| |
|
| Contributor(s) |
Park S, Thornton AM, Cho H, Barlowe TS, Chou T, Chhangawala S, Fairchild L, Taggart J, Chow A, Schurer A, Witkin MD, Shevach EM, Kristov A, Armstrong SA, Leslie C, Kharas MG |
| Citation(s) |
30472158 |
| |
| Submission date |
Dec 20, 2017 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Hyein Sophia Cho |
| E-mail(s) |
[email protected]
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Developmental Biology
|
| Street address |
430 E 67th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE120630 |
IKZF2 is required for myeloid leukemic stem cells by driving self-renewal and inhibiting the myeloid differentiation program |
|
| Relations |
| BioProject |
PRJNA423273 |
| SRA |
SRP127266 |
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