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| Status |
Public on Jun 02, 2008 |
| Title |
Response of Arabidopsis cell culture to phytoprostane A1 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
12-Oxo-phytodienoic acid (OPDA) and several phytoprostanes are structurally related cyclopentenone oxylipins that can be formed via the enzymatic jasmonate pathway and a non-enzymatic, free radical-catalyzed pathway, respectively. To elucidate the biological activities of phytoprostanes in comparison to OPDA as well as the metabolism we performed genome-wide expression analysis.
Keywords: mixotrophic Arabidopsis cell culture, ATH1 Chip
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| Overall design |
In order to get a comprehensive view of the biological activity of non-enzymatically formed reactive oxylipins the regulation of gene expression in Arabidopsis thaliana was analyzed using the ATH1 chip representing 22500 genes. A mixotrophic cell culture of Arabidopsis was treated with 75 µM phytoprostane A1 or with 0.5% methanol in water (Control) and harvested after 4 h. Three independent replicates of each treatment were analyzed and the fold change of normalized signals from PPA1-treated cell culture versus control cell culture was calculated.
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| Citation(s) |
18334669 |
| |
| Submission date |
Mar 04, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Susanne Berger |
| E-mail(s) |
[email protected]
|
| Phone |
49 931 318 6170
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| Organization name |
Julius-von-Sachs-Institute
|
| Department |
Pharmaceutical Biology
|
| Street address |
Julius-von-Sachs-Platz 2
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| City |
Wuerzburg |
| ZIP/Postal code |
97082 |
| Country |
Germany |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM270814 |
Arabidopsis cell culture, 4 h_control_rep1 |
| GSM270853 |
Arabidopsis cell culture, 4 h_control_rep2 |
| GSM270865 |
Arabidopsis cell culture, 4 h_control_rep3 |
| GSM270866 |
Arabidopsis cell culture, 4 h_response to phytoprostane A1_rep1 |
| GSM270868 |
Arabidopsis cell culture, 4 h_response to phytoprostane A1_rep2 |
| GSM270870 |
Arabidopsis cell culture, 4 h_response to phytoprostane A1_rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE10749 |
Response of Arabidopsis cell culture to cyclopentenone oxylipins |
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| Relations |
| BioProject |
PRJNA108855 |
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