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| Status |
Public on Jun 08, 2018 |
| Title |
Exosomal microRNA (miRNA) expression in Blonchoalveolar lavage fluid (BALF) collected from mice infected with influenza virus and inoculated with poly(I:C). |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To clarify the profile of in BALF exosome collected from mice infected with influenza virus, we infected 100000 pfu of A/Puerto Rico/8/1934 (PR8) strain. BALF was collected at 24, 48, and 72 hour post infection (hpi). For comparison of the profile of the miRNA in BALF exosome induced by innate immune response, we also intranasally inoculated mice with 50 μg of poly(I:C) and collected BALF at 72 hour post inoculation. We found that some miRNAs were common to both influenza virus infectiona and poly(I:C) inoculation, suggesting that exosomal miRNAs in BALF may function in the innate immune response to virus infection.
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| Overall design |
Each group had three biological replication. Exosome was isolated by using filteration and ultracentrifugation. BALF exosome isolated from naive mice was used as control. After isolation of total RNA, miRNA microarray was performed.
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| Contributor(s) |
Maemura T, Fukuyama S, Lopes TJ, Kawaoka Y |
| Citation(s) |
29373693 |
| |
| Submission date |
Nov 17, 2017 |
| Last update date |
Jun 10, 2018 |
| Contact name |
Tadashi Maemura |
| E-mail(s) |
[email protected]
|
| Organization name |
Influenza Research Institute
|
| Street address |
575 Science Drive
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
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| Platforms (1) |
| GPL17912 |
Agilent-046065 Mouse_miRNA_V19.0_Microarray (miRNA ID version) |
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| Samples (15)
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| GSM2861393 |
BALF_exosome_PR8_infection_24hpi_rep1 |
| GSM2861394 |
BALF_exosome_PR8_infection_24hpi_rep2 |
| GSM2861395 |
BALF_exosome_PR8_infection_24hpi_rep3 |
| GSM2861396 |
BALF_exosome_PR8_infection_48hpi_rep1 |
| GSM2861397 |
BALF_exosome_PR8_infection_48hpi_rep2 |
| GSM2861398 |
BALF_exosome_PR8_infection_48hpi_rep3 |
| GSM2861399 |
BALF_exosome_PR8_infection_72hpi_rep1 |
| GSM2861400 |
BALF_exosome_PR8_infection_72hpi_rep2 |
| GSM2861401 |
BALF_exosome_PR8_infection_72hpi_rep3 |
| GSM2861402 |
BALF_exosome_poly(I:C)_inoculation_72h_rep1 |
| GSM2861403 |
BALF_exosome_poly(I:C)_inoculation_72h_rep2 |
| GSM2861404 |
BALF_exosome_poly(I:C)_inoculation_72h_rep3 |
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| Relations |
| BioProject |
PRJNA418999 |
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