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| Status |
Public on Feb 05, 2018 |
| Title |
The single cell RNA seq of pulmonary alveolar epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The pulmonary alveolar epithelium which play key role in lung biological function is mainly composed of two types of epithelial cells: alveolar type I (AT1) and type II (AT2) cells. We know very little about developmental heterogeneity of the AT1 cell population. By using 10X genomics “Chromium Single Cell” technology, we performed single-cell RNA-seq (scRNA-seq) analyses of AT1 cells at postnatal day 3 (P3), P15, and P60, along with AT2 cells (P60) in mice. Our study identified a robust new genetic marker (Igfbp2) of postnatal AT1 cells. The study also provided the transcriptome information of AT1 cells during alveologensis.
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| Overall design |
We performed 10X genomics single-cell RNA-seq at various developmental stages of AT1 cells of lungs at postnatal (P)3, P15, and P60. We also performed 10X genomics single-cell RNA-seq of AT2 cells of P60 lungs.
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| |
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| Contributor(s) |
Wang Y, Tang N, Cai T |
| Citation(s) |
29463737 |
| |
| Submission date |
Nov 15, 2017 |
| Last update date |
Mar 31, 2020 |
| Contact name |
huanwei huang |
| E-mail(s) |
[email protected]
|
| Organization name |
NIBS
|
| Street address |
7 Science Park Rd., ZGC life science park
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA418587 |
| SRA |
SRP125061 |
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