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| Status |
Public on Jan 22, 2019 |
| Title |
ChIP of PolII from cells with Mago knockdown, control knockdown, and RnpS1 knockdwon [Promoter-proximal pausing mediated by the exon junction complex regulates splicing] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Promoter-proximal pausing of RNA polymerase II (Pol II) is a widespread transcriptional regulatory step across metazoans. Here we find that the nuclear exon junction complex (pre-EJC) is a critical and conserved regulator of this process. Depletion of pre-EJC subunits leads to a global decrease in Pol II pausing and to premature entry into elongation. This effect occurs, at least in part, via non-canonical recruitment of pre-EJC components at promoters. Failure to recruit the pre-EJC at promoters results in increased binding of the positive transcription elongation complex (P-TEFb) and in enhanced Pol II release. Notably, restoring pausing is sufficient to rescue exon skipping and the photoreceptor differentiation defect associated with depletion of pre-EJC components in vivo. We propose that the pre-EJC serves as an early transcriptional checkpoint to prevent premature entry into elongation, ensuring proper recruitment of RNA processing components that are necessary for exon definition.
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| |
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| Overall design |
Pol II ChIP in control and Mago depleted S2R+ cells, in duplicates
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| |
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| Contributor(s) |
Akhtar J, Kreim N |
| Citation(s) |
30705266 |
| |
| Submission date |
Nov 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jean-Yves Roignant |
| Organization name |
Institute of molecular Biology
|
| Lab |
Roignant
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE92389 |
Promoter-proximal pausing mediated by the exon junction complex regulates splicing |
|
| Relations |
| BioProject |
PRJNA418419 |
| SRA |
SRP125013 |
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