To obtain samples for RNA analysis following treatment of primary human lung fibroblasts (FB)) with universal interferon alpha beta or interferon gamma.
Overall design
Human lung fibroblasts cells were infected with a multiplicity of 4 PFU per cell. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 3, 6, and 24 hrs, post-infection. MERSCOV003.0P references the write up of the experimental procedure.