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| Status |
Public on Dec 04, 2018 |
| Title |
RNA-Sequencing of Control KD and KD for Ime4-dMettl14, Fl(2)d, Vir, Nito, CG7358 in S2R+ cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
N6-methyladenosine (m6A) is an abundant RNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Flacc/Zc3h13 as a novel interactor of m6A methyltransferase complex components in Drosophila and mouse. Like other components, Flacc controls m6A levels and is involved in sex determination in Drosophila. We demonstrate that Flacc promotes the recruitment of the methyltransferase to mRNA by bridging Fl(2)d to the mRNA binding factor Spenito. Altogether, our work advances our molecular understanding of conservation and regulation of the m6A machinery.
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| Overall design |
Cells were treated with corresponding double stranded RNA three times during 6 days
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| Contributor(s) |
Lence T, Kreim N, Roignant J |
| Citation(s) |
29535189, 34145251 |
| |
| Submission date |
Oct 23, 2017 |
| Last update date |
Jul 08, 2021 |
| Contact name |
Jean-Yves Roignant |
| Organization name |
Institute of molecular Biology
|
| Lab |
Roignant
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE106614 |
Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to other components of the m6A machinery |
|
| Relations |
| BioProject |
PRJNA415477 |
| SRA |
SRP121013 |
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