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| Status |
Public on Feb 13, 2018 |
| Title |
Transcriptome analysis of murine macrophages infected with different strains of Leptospira spp |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Leptospirosis is a neglected zoonotic disease of global importance. Despite its prevalence, pathogenesis is still poorly understood. Our aim was to discover transcripts responsable for pathogenicity of leptospirosis. We compared the transcriptome profiles of saprophyte, attenuated and virulent strain of Leptospira spp.
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| Overall design |
Four treatments were performed in triplicates in J774A.1 macrophages in vitro, as follows: non-infected control cells and cells infected with virulent, attenuated and saprophyte strains of Leptospira. Following 6h of infection, total RNA was extracted and 250 ng of total RNA (RIN 10) was used to evaluate the mRNA profile using the microarray Affymetrix® Mouse Gene 2.1 ST Array Strips. Raw intensity values were background corrected, log2 transformed and then quantile normalized by the software Expression Console (Affymetrix) using the Robust Multi-array Average (RMA-Sketch) algorithm. Next, statistical analysis was performed in the TAC software (Affymetrix) by ANOVA (fold change ± 2, FDR-corrected p<0.01).
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| Contributor(s) |
Júnior EC, Garcia LE, Peiró JR, Arnold DR, Marinho M, Lopes FL |
| Citation(s) |
29326966 |
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| Submission date |
Oct 18, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Flavia Lombardi Lopes |
| E-mail(s) |
[email protected]
|
| Phone |
551836360032
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| Organization name |
FMVA-UNESP
|
| Department |
DAPSA
|
| Lab |
Epigenomics Lab
|
| Street address |
Rua Clovis Pestana, 793
|
| City |
Araçatuba |
| State/province |
São Paulo |
| ZIP/Postal code |
16050-680 |
| Country |
Brazil |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM2823126 |
Macrophages infected with saprophyte strain_6h_rep1 |
| GSM2823127 |
Macrophages infected with saprophyte strain_6h_rep2 |
| GSM2823128 |
Macrophages infected with saprophyte strain_6h_rep3 |
| GSM2823129 |
Macrophages infected with attenuated strain_6h_rep1 |
| GSM2823130 |
Macrophages infected with attenuated strain_6h_rep2 |
| GSM2823131 |
Macrophages infected with attenuated strain_6h_rep3 |
| GSM2823132 |
Macrophages infected with virulent strain_6h_rep1 |
| GSM2823133 |
Macrophages infected with virulent strain_6h_rep2 |
| GSM2823134 |
Macrophages infected with virulent strain_6h_rep3 |
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| Relations |
| BioProject |
PRJNA414919 |
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