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| Status |
Public on Sep 20, 2017 |
| Title |
Measuring RNA editing through mmPCR-seq in Drosophila adapting to divergent microclimates and raised at different temperatures |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
We applied microfluidic multiplex PCR and deep sequencing (mmPCR-seq) to quantify RNA editing levels at targeted sites in 32 isofemale lines from two divergent microclimates at 'Evolution Canyon' in Israel (16 fly lines from each microclimate). Editing levels were compared between different populations at 25˚C , and were also compared between 25˚C and 18˚C within populations.
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| Overall design |
For each of the 32 isofemale fly lines from 'Evolution Canyon', quantification of RNA editing levels in the whole bodies of 3-5 day old male flies raised at 25˚C or 18˚C through RT-PCR amplification of 605 loci. Two biological replicates are represented per fly line.
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| Contributor(s) |
Yablonovitch AL, Li K, Rashkovetsky E, Korol AB, Michalak P, Nevo E, Li JB |
| Citation(s) |
29146998 |
| |
| Submission date |
Sep 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jin Billy Li |
| E-mail(s) |
[email protected]
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| Organization name |
Stanford University
|
| Department |
Genetics
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| Street address |
300 Pasteur Drive, Alway M-341
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (2) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (128)
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| This SubSeries is part of SuperSeries: |
| GSE104085 |
Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates |
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| Relations |
| BioProject |
PRJNA408169 |
| SRA |
SRP118387 |
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