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| Status |
Public on Jan 11, 2021 |
| Title |
Global transcriptome analysis of Stenotrophomonas maltophilia in response to human body temperature |
| Organism |
Stenotrophomonas maltophilia |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stenotrophomonas maltophilia is an emerging multidrug resistance opportunistic pathogen affecting immunocompromised and hospitalized patients. S. maltophilia is an environmental bacterium which adapts to human body and causing infection. S. rhizophilia, a non-pathogenic and phylogenetic neighbour of S. maltophilia is unable to grow at human body temperature. Thus, to understand molecular mechanism underlying the adaptation of S. maltophilia at human body temperature, we performed the comparative transcriptome analysis of S.maltophilia at 28 °C (representative for the environmental niches) and 37 °C (representative for human body) by using RNA-Seq. The major temperature-induced genes include genes for Type IV secretion system, aerotaxis, and cation diffusion facilitator family transporter suggesting its potential role in the adaptation and virulence of S. maltophilia. The downregulated genes at 37 °C includes the genes for the cell motility, energy generation and metabolism, lipid metabolism, translation, amino acid metabolism and transport, replication and repair, inorganic ion and transport metabolism lipid metabolism, coenzyme metabolism.
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| Overall design |
Comparative transcriptome analysis of S.maltophilia at 28°C as a temperature representative for the environmental niches and 37 °C for the human body by using RNA-Seq. Total RNA was isolated by growing the cells at 28°C and 37°C in triplicate, ribodepleted RNA samples, Illumina library constructed of cDNA and sequenced on Illumina Miseq.
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| Contributor(s) |
Patil PP, Kumar S, Midha S, Patil PB |
| Citation(s) |
34252021 |
| BioProject |
PRJNA395418 |
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| Submission date |
Jul 26, 2017 |
| Last update date |
Oct 06, 2021 |
| Contact name |
Prabhu B Patil |
| E-mail(s) |
[email protected]
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| Organization name |
CSIR Institute of Microbial Technology Chandigarh
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| Lab |
Bacterial Genomics and Evolution
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| Street address |
Sector 39 A
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| City |
Chandigarh |
| State/province |
Chandigarh |
| ZIP/Postal code |
160036 |
| Country |
India |
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| Platforms (1) |
| GPL23835 |
Illumina MiSeq (Stenotrophomonas maltophilia) |
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| Samples (6)
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