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Series GSE10135 Query DataSets for GSE10135
Status Public on Jan 06, 2009
Title Cigarette Smoking Induces Overexpression of a Fat Depleting Gene AZGP1 in the Human Airway Epithelium
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Smokers weigh less and have less body fat than non-smokers, and increased body fat and weight gain are observed following smoking cessation. To assess a possible molecular mechanism underlying the inverse association between smoking and body weight, we hypothesized that smoking may induce the expression of a fat depleting gene in the airway epithelium, the cell population that takes the brunt of the stress of cigarette smoke. As a candidate gene we evaluated the expression of alpha2-zinc-glycoprotein1 (AZGP1), a soluble protein that stimulates lipolysis, induces a reduction in body fat in mice and is associated with the cachexia related to cancer, and is known to be expressed in secretory cells of lung epithelium. To assess if smoking upregulates AZGP1 expression, microarray analysis with TaqMan confirmation was used to evaluate large airway epithelial samples obtained by fiberoptic bronchoscopy from 37 normal smokers and 55 normal nonsmokers. Both microarray and TaqMan analysis demonstrated that AZGP1 mRNA levels were higher in the large airway epithelium of normal smokers compared to normal nonsmokers (p<0.05, all comparisons). Western analysis of airway biopsies of smokers compared with nonsmokers demonstrated upregulation of AZGP1 at the protein level, and immunohistochemical analysis demonstrated upregulation of AZGP1 in secretory as well as neuroendocrine cells of smokers. In the context that AZGP1 is involved in lipolysis and fat loss, its overexpression in the airway epithelium of chronic smokers may represent one mechanism for the weight difference in smokers vs nonsmokers.
Keywords: Comparison of gene expression in large airway epithleium of normal non-smokers and normal smokers
 
Overall design Airway epithelial cells obtained by bronchoscopy with brushing from healthy smokers and healthy non-smokers
 
Contributor(s) Vanni H, Kazeros A, Harvey B, Ferris B, De BP, Carolan BJ, Hübner R, Crystal RG
Citation(s) 19188554
Submission date Jan 10, 2008
Last update date Mar 25, 2019
Contact name Yael Strulovici-Barel
E-mail(s) [email protected]
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platforms (3)
GPL80 [Hu6800] Affymetrix Human Full Length HuGeneFL Array
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (92)
GSM114035 large airways, non-smoker 010119R, MAS5
GSM114037 large airways, non-smoker 010316R, MAS5
GSM114039 large airways, non-smoker 010430R, MAS5
Relations
BioProject PRJNA108683

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10135_RAW.tar 1.2 Gb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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