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| Status |
Public on Sep 16, 2017 |
| Title |
CD95L derived si- and shRNAs kill cancer cells through an RNAi mechanism by targeting survival genes [shL1.RNAseq.lg] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We provide evidence that shRNAs and siRNAs derived from CD95 and CD95L preferentially target the 3' UTRs of survival genes culminating in a very robust mode of cell death we call DISE (Death Induced by Survival gene Elimination)
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| |
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| Overall design |
293T cells were infected with either pTIP-shScr or pTIP-shL1 and following puromycin selection RNA was analyzed by deep sequencing 100hrs after addition of doxycycline
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| |
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| Contributor(s) |
Putzbach W, Peter ME, Bartom E |
| Citation(s) |
29063830 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 CA197450 |
DISE - a natural cancer surveillance mechanism - a new road to cancer therapy |
NORTHWESTERN UNIVERSITY AT CHICAGO |
Marcus E. Peter |
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| |
| Submission date |
Jul 11, 2017 |
| Last update date |
Sep 08, 2022 |
| Contact name |
Marcus Peter |
| E-mail(s) |
[email protected]
|
| Organization name |
Northwestern University Feinberg School of Medicine
|
| Street address |
303 East Superior Street, Lurie 6-123
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (4)
|
| GSM2700022 |
293T-pTIP-shScr + Dox for 100 hrs Replicate 1 |
| GSM2700023 |
293T-pTIP-shScr + Dox for 100 hrs Replicate 2 |
| GSM2700024 |
293T-pTIP-shL1 + Dox for 100 hrs Replicate 1 |
| GSM2700025 |
293T-pTIP-shL1 + Dox for 100 hrs Replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE87817 |
CD95L derived si- and shRNAs kill cancer cells through an RNAi mechanism by targeting survival genes |
|
| Relations |
| BioProject |
PRJNA393824 |
| SRA |
SRP111526 |
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