|
| Status |
Public on Aug 12, 2017 |
| Title |
Transcriptome sequencing identified RNAs associated with renal fibrosis in UUO rat model (kidney) |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Renal fibrosis as the final outcome of many renal diseases, has always been paid more attention to by the researchers. To better understand whether lncRNAs could be a player in this process or be a biomarker for renal fibrosis diagnosis, we compared transcriptome sequencing data on renal tissues and urine respectively between UUO and shamed (Sham) rat model. Numerous genes including lncRNAs with significant changes in their expression were identified. 24 lncRNAs were up-regulated and 79 lncRNAs were down-regulated in the renal tissues of the UUO rats. 625 lncRNAs were up-regulated and 177 lncRNAs were down-regulated in urines of the UUO rats. Among the lncRNAs upregulated in renal tissue of UUO rats, 19 lncRNAs were predicted containing several conserved Smad3 binding motifs in the promoter. Among them, lncRNAs with putative promoter containing more than 4 conserved Smad3 binding motifs were demonstrated to be induced by TGF-β significantly in NRK-52E cells. We further confirmed that lncRNA TCONS_00088786 and TCONS_01496394 were regulated by TGF-β stimulation and also can influence the expression of some fibrosis-related genes through a feedback loop. Based on transcriptome sequencing data, bioinformatics analysis and qRT-PCR detection, we also demonstrated lncRNA in Urine are detectable and might be a novel biomarker of renal fibrosis. These data provide new information about the involvement of lncRNAs in renal fibrosis, indicating that they may serve as candidate biomarkers or therapeutic targets in the future.
|
| |
|
| Overall design |
Rat RNA profiles of 14-day wild type (WT) and UUO rat model type were generated by deep sequencing, in triplicate, using Illumina HI-seq 2500.
|
| |
|
| Contributor(s) |
Sun J, Zhang S, Shi B, Zheng D, Shi J |
| Citation(s) |
28912732 |
| |
| Submission date |
Jun 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiazeng Sun |
| E-mail(s) |
[email protected]
|
| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Street address |
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China
|
| City |
Beijing |
| State/province |
None Selected |
| ZIP/Postal code |
100005 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE100633 |
Transcriptome sequencing identified RNAs associated with renal fibrosis in UUO rat model |
|
| Relations |
| BioProject |
PRJNA392328 |
| SRA |
SRP110710 |
Less...
More...