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| Status |
Public on May 10, 2018 |
| Title |
Transcriptomic analysis of neuroepithelium and sorted neural progenitors in the murine cortex duirng early stages of development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Relatively little is known about how the identity of early neuronal stem cells changes before and after neural tube closure (neurulation). We performed RNA sequencing on microdissected forebrain precursors and revealed sharp reductions in expresion of protein biosynthetic machinery after neurulation. These reductions were paralleled by down-regulation of Myc, which regulated forebrain precursor ribosome ribosome biogenesis. To study consequences of Myc dysregulation, we overexpressed Myc in Nestin+ neural progenitors, sorted these progenitors for RNA sequencing, and identified 135 genes that are differentially expressed between Myc-overexpressed embryos and their wildtype littermates.
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| Overall design |
The first RNA sequencing dataset contains micordissected neuroepithelium from E8.5 and E10.5 mouse embryos, two biological replicates for each age.
The second RNA sequencing dataset contain FACS isolated Pax6+ neural progenitors form the cortex of E13.5 MYC-overexpressed embryos and their wildtype littermates, three biological replicates for each genotype.
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| Contributor(s) |
Chau KF, Johnson MB, Springel MW, Lehtinen MK |
| Citation(s) |
29745900 |
| |
| Submission date |
Jun 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Fongching Chau |
| Organization name |
Boston Children's Hospital
|
| Lab |
Maria Lehtinen
|
| Street address |
Enders Building. Room 1150, 300 Longwood Avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA391702 |
| SRA |
SRP110277 |
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