Microarray Centre University Health Network Toronto Medical Discovery Tower 101 College Street, Rm 9-301 Toronto, Ontario, M5G 1L7
Manufacture protocol
1. Acquisition of bacterial clone set - our Yeast 6.4k clone set is derived from Research Genetics Genepairs, Detailed information about the ORFs and sequences can be found at http://www.yeastgenome.org/ 2. Clone set is PCR amplified and DNA purified in working 96 well plates. 3. The purified DNA is then transferred to 384 well source plates with DNA concentration ranging from .10ug/ul to 0.25ug/ul. These are used for spotting. Microarrays are printed on UltraGAPS™ slides (Corning Inc.) using high-precision robotics. Printed arrays are processed following Corning's protocol and are ready-to-use. Microarray Layout Specifications Grid Rows12 Grid Cols 4 Rows 17 Cols 16 Grid to Grid Distance (um) 4500 Spot to Spot Distance (um) 200
Catalog number
Y6.4K
Support
glass
Description
48 subarrays (spots organised in 17 rows and 16 columns per subarray) distributed in 12 rows and 4 columns of subarrays. Controls are 3XSSC and Arabidopsis
The Platform data table reflects a condensed representation of the array's duplicate features. The full, uncondensed array is deposited under GPL764.
Characterization of sensor-specific stress response by transcriptional profiling of wsc1 and mid2 deletion strains and chimeric sensors in Saccharomyces cerevisiae
Less...
More...