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Platform GPL3412 Query DataSets for GPL3412
Status Public on Mar 17, 2006
Title Li UCSF yeast 14062 v1.0
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Saccharomyces cerevisiae
Manufacturer UCSF Li Lab
Manufacture protocol PCR products representing ORF and intergenic yeast genomic sequences were amplified. Products were spotted on poly-L-lysine coated glass slides using a linear servo arrayer from the DeRisi Lab at UCSF.

Blast analysis of oligos sequences was used to determine the position of the final PCR product. Those oligo pairs which Blast analysis indicated could not generate a product or generated more than one product given the flag –1. In cases where no product was found the fields Chr, Start and End have been left empty. In cases where multiple products were found values in the fields Chr, Start and End correspond to the first product identified.

For all elements (except mitochondrial) the sequence of the PCR product was determined using the positions determined above. Blast analysis of the PCR products was used to determine how repetitive each product was. Elements that had products that were too repetitive were given a flag of –2. The sequence of these products is listed.

Elements with flags of –1 or –2 were removed from all datasets prior to analysis.

Lamda DNA spots were not included in analysis.
Support glass
Coating polysine
 
 
Contributor(s) Morreale RJ, Green BM, Ozaydin B, Derisi JL
Submission date Feb 01, 2006
Last update date Mar 17, 2006
Contact name Joachim J Li
E-mail(s) [email protected]
Phone 415-476-8782
Fax 415-514-4080
Organization name University of California, San Francisco
Department Microbiology and Immunology
Lab Li
Street address 600 16th Street S376
City San Francisco
State/province CA
ZIP/Postal code 94143-2200
Country USA
 
Samples (1083) GSM94891, GSM95255, GSM95257, GSM95258, GSM95352, GSM95353 
Series (6)
GSE4181 Genome-wide mapping of DNA re-replication in S. cerevisiae
GSE22018 Loss of DNA replication control is a potent initiator of gene amplification
GSE41259 Single-Stranded Annealing Induced by Re-Initiation of Replication Origins Provides a Novel and Efficient Mechanism for Generating Copy Number Expansion via Non-Allelic Homologous Recombination.

Data table header descriptions
ID
Flag 0: Good, element included in analysis, -1: Bad, oligos do not generate a single unique PCR product, -2: Bad, sequence of PCR product too repetitive
Name SGD name of ORF
ORF SGD Gene
SPOT_ID
Chromosome Chromosome of element (1-16, Mito)
Start Starting chromosomal position of element (bp from left end)
End Ending chromosomal position of element (bp from left end)
SEQUENCE Sequence of PCR product on the array

Data table
ID Flag Name ORF SPOT_ID Chromosome Start End SEQUENCE
15S_rRNA_2 0 15S_rRNA_2 Mito 6546 8194
15S_rRNA1 0 15S_rRNA1 Mito 6591 7255
15S_rRNA2 0 15S_rRNA2 Mito 7373 8177
21S_rRNA_3 0 21S_rRNA_3 Mito 58009 60724
21S_rRNA_4 0 21S_rRNA_4 Mito 61868 62447
21S_rRNA0 0 21S_rRNA0 Mito 61932 62585
21S_rRNA1 0 21S_rRNA1 Mito 58042 59319
21S_rRNA2 0 21S_rRNA2 Mito 59350 60703
9S_rRNA 0 9S_rRNA Mito 85311 85778
9S_rRNA_5 0 9S_rRNA_5 Mito 85290 85779
CEN1 0 CEN1 1 151438 151605 CTTTTATGTAATGATTTAAGTCTTGTCACATGACATAATAATAAATAATTTTAAAAATATAAAATATTTTTAATAGTTTTTAAATATTTTACAGTTTATTTTTTAAATTTATTTATATGTTTTTGTTTTCCGAAGCAGTCAAAGTATTTTAATTTTCGGAGCTTTCAT
CEN10 0 CEN10 10 436002 436126 GTTCGGAAATCATAAACATTGTTTTTTTGTGTTTTTTATTTTTAAATAAAAAGAATAAATATTTTATATTAAAAAATAAATTTTAAAGTAAATTAATTATTTAACACGTGATTAAGTTACTCTAA
CEN11 0 CEN11 11 439775 439889 TTCGGAAATCATGAACATTTATTTTTATGATTATGTAATTAAAAAAATATAATAAATTTATTTTGAAAATTAATTTTTTTAAAATTTTAAATATGTTTTTATCATGTGACTTATG
CEN12 0 CEN12 12 150832 150958 TTGTTCGGATAACAAATACAATAGTGTTATTAAAAAATAAAACTTATTTAAAAATAGTAATTTAAATTATTATTTTATTTTAATAAACTTTTTAATAATATTTATTACACGTGATTTAATATATCCT
CEN13 0 CEN13 13 268020 268135 CGTGACAAAAAATCACATGACTACCTAACAAAATATTTATTTTTCTTTTTTAATATTTGAAAATACTAAAATATTTTTGTTGTTTTTTGAAAAAAGGATTTTTAATGTGTATGCGT
CEN14 0 CEN14 14 628747 628878 TCTAGTTAGTCACGTGCAGCTTTTTAAAAATATTTTAAAACATTTTAAAAAATATACATTTTTTTATTATTTTTTTATATATTAATGTTAAAATTTATTTATGTATTTGTCTTCCGAAAAGTAAAATAACGT
CEN15 0 CEN15 15 326592 326699 TTCGGAAGTCATATACATTAAATAATTAAAACATTATTTTTAAAAATTAAAAAAATATATTTTAGCAAATAGTTTTTACTTTTTTTTAAATGCAAAATAAGTTCACGT
CEN16 0 CEN16 16 555940 556056 GATCACAAATAGATCACATGATATATTTTTTATTTTTAATTTTTTTTAATTATAAAAATAATTTTTTTCTTTAAATTAAACAAAAATAAAAAATTGTTTTTTGTTGGTTAAGATTTC
CEN2 0 CEN2 2 238154 238298 ATTGATCCTACATAAATCATGTGACTTATTTATTTAATTATTATTAAGTAAAAAAGATTTTCTATTTAAATTTATTAATTAATTTTTTTTCTTAAATAATTATTTTATGTTTTTGTTTTCCGAAAAAGAAAAATATTTCAACAAG
CEN3 0 CEN3 3 113972 114099 AATAGTACAAATAAGTCACATGATGATATTTGATTTTATTATATTTTTAAAAAAAGTAAAAAATAAAAAGTAGTTTATTTTTAAAAAATAAAATTTAAAATATTAGTGTATTTGATTTCCGAAAGTTA

Total number of rows: 14062

Table truncated, full table size 12050 Kbytes.






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