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Platform GPL20190 Query DataSets for GPL20190
Status Public on Jul 31, 2015
Title NCSU C. bescii 3K v1.0
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Caldicellulosiruptor bescii
Manufacturer NCSU Kelly Group
Manufacture protocol A DNA microarray was designed and constructed based on 2674 protein-coding sequences in the C. bescii genome obtained from the Department of Energy's Joint Genomics Institute (https://img.jgi.doe.gov). OligoArray 2.1 (Rouillard, 2003) was used to generate one 30 to 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted five times onto each array to fortify statistical analysis.
 
 
Submission date May 12, 2015
Last update date May 13, 2015
Contact name Jeffrey Zurawski
Organization name North Carolina State University
Department Chemical and Biomolecular Engineering
Lab Robert Kelly
Street address 840 Main Campus Drive Room 3309
City Raleigh
State/province NC
ZIP/Postal code 27606
Country USA
 
Samples (1) GSM1682221
Series (1)
GSE68810 Comparative analysis of extremely thermophilic Caldicellulosiruptor species reveals common and differentiating cellular strategies for plant biomass utilization

Data table header descriptions
ID
ORF ORF Name
Gene ID IMG Gene ID
SEQUENCE Microarray Probe Sequence

Data table
ID ORF Gene ID SEQUENCE
Athe_0001 Athe_0001 643714415 CAAGAAGGTCAAGAAACGTAGCATTCCCGCGACAGATAGCTATGTATTTAGCAAGGGAAC
Athe_0002 Athe_0002 643714416 TAACCTCAAATTTCACAAGCTTTTGACACTGTTATTAACTTATCCACAGCTATTACTGTG
Athe_0003 Athe_0003 643714417 CAAAAGCCAGACGACCCTAACTACATCTACCTTGTTCTTCCTATAAAGATGCCAGAGTAA
Athe_0004 Athe_0004 643714418 TCTGCAGAAGATTTAAGTGTTCTTGAAAGAGAAGAAATACTGGAGTTTGGCAGTGCAAAC
Athe_0005 Athe_0005 643714419 TATCATAATCCTTGAGGTTGATGGAAGAATGTACGGAATAATATCTCCTGTGTCGCCGGG
Athe_0006 Athe_0006 643714420 GGTAGAGCCAAGAAGGGAGTTTATTGAAAAGAATGCTAAGTATGTAAGGAATTTGGATAT
Athe_0007 Athe_0007 643714421 TAAGAAGTTATAAAACTGCCTGAAGAGACTCAGAGAGAAGTTTTGGATTTTATTGAATTT
Athe_0008 Athe_0008 643714422 CAGTTCAGGGAAGAAATACACAGGGTGTTAAACTTATGCGAATTGATGGAGATAATATAA
Athe_0009 Athe_0009 643714423 TGGTGATGTGCTCTACTCAGTTTCAAACTCAATGATAAAAGCAAGCTCTCTTGAAAGCTT
Athe_0010 Athe_0010 643714424 TGCGTTATTGTTCCAGTTTTAACGGCTACATATCAAAGTAAAAGTTATTTAGAAACTACA
Athe_0011 Athe_0011 643714425 TTTTTTTTTTGAAAAGTGAAGAAAAATGACCTTGGCATGGGAATTTACATTTGCGAATAA
Athe_0012 Athe_0012 643714426 TTTTAGCTGGAGCATACAATCAAAGATGGGAGCTGATTTAAACTTAGGGGCGGTAAGAGG
Athe_0013 Athe_0013 643714427 CATACTACTGTGATGGCAATGAATCAGAAAGAATGTTATTGGTAGACGCAATTAAAGAAG
Athe_0014 Athe_0014 643714428 GGGGAGAGGAAGGAATTGAAGATGAAGATGAAAGGAAGATATATGAGAAAATAAAAGAAG
Athe_0015 Athe_0015 643714429 TTATCTTATCTTCTTAAAAGGTATGACATTATAACTAGGTCTGATGAAGGGGATTTATGA
Athe_0016 Athe_0016 643714430 CAGGGAAAATAAGAAAGCTGAGGTAAAAGAAGGAGGTATATTTGTAAAAGACGACGAAAA
Athe_0017 Athe_0017 643714431 CGGCACGTAACGGAAGATGACAAAATTACTCTTTCAGAAAATTTAATAGAATTGACCTCA
Athe_0019 Athe_0019 643714432 ATGGTGAAGCATATGATTTTGTCTACAGAGTATTAGAAAGCGAGATAATAGAAATGGGTG
Athe_0020 Athe_0020 643714433 TGCGCACAGTGTTGAAAGATTTCTAATTATATGCATTATATTAGTATTAATTGCAGGTGT
Athe_0022 Athe_0022 643714434 CCAATAAACATCAAAGAAGGGGAACTTCGTATAGTTGGAAAAGTTGTCGGCGTAATTAGG

Total number of rows: 2674

Table truncated, full table size 237 Kbytes.




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Supplementary data files not provided
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