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Platform GPL1474 Query DataSets for GPL1474
Status Public on Oct 01, 2004
Title NIEHS Yeast Chip v1.0
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Saccharomyces cerevisiae
 
Description This cDNA Yeast Chip was developed in-house at NIEHS. A complete listing of the 6,218 open reading frames on this chip is available at the following website: https://tools.niehs.nih.gov/maps/index.cfm
cDNA microarray chips were prepared as previously described (DeRisi, Penland et al. 1996; Hauser, Vingron et al. 1998). The spotted cDNAs were derived from a collection of primers of yeast ORFs (Research Genetics, Carlsbad, CA). These primers were used to amplify yeast cDNAs from genomic DNA in a 100µl PCR reaction mixture. A sample of the PCR products (10 ul) was separated on 2% agarose gels to ensure quality of the amplifications. The remaining PCR products were purified by ethanol precipitation, resuspended in ArrayIt Spotting Solution buffer (Telechem, San Jose CA) and spotted onto poly-L-lysine coated glass slides using a modified, robotic DNA arrayer (Beecher Instruments, Bethesda MD).
 
Web link https://tools.niehs.nih.gov/maps/index.cfm
Submission date Sep 28, 2004
Last update date Mar 25, 2022
Contact name NIEHS Microarray Core
E-mail(s) [email protected], [email protected]
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Samples (20) GSM31147, GSM31173, GSM31174, GSM31175, GSM31176, GSM31177 
Series (1)
GSE1798 Integrating phenotypic and expression profiles to map arsenic response networks

Data table header descriptions
ID
PenAt Pen position (left to right)
RowAt Within each subarray (pen), row offset
ColumnAt Within each subarray (pen), column offset
Description Gene Name
ORF ORF number;
PlatePos Plate/row/column of source plate
FeatureID ID of feature on array (eeeee=empty well)
SPOT_ID

Data table
ID PenAt RowAt ColumnAt Description ORF PlatePos FeatureID SPOT_ID
1 1 1 1 transcription factor tau (TFIIIC) subunit 138 YAL001C 1A1 YAL001C
2 2 1 1 Cell wall protein involved in flocculation YAR050W 2A1 YAR050W
3 3 1 1 YAL002W 1A2 YAL002W
4 4 1 1 YAR052C 2A2 YAR052C
5 1 1 3 Translation elongation factor EF-1beta, GDP\/GTP exchange factor for Tef1p\/Tef2p YAL003W 1A3 YAL003W
6 2 1 3 YAR053W 2A3 YAR053W
7 3 1 3 YAL004W 1A4 YAL004W
8 4 1 3 YAR060C 2A4 YAR060C
9 1 1 5 Heat shock protein of HSP70 family, cytoplasmic YAL005C 1A5 YAL005C
10 2 1 5 YAR061W 2A5 YAR061W
11 3 1 5 YAL007C 1A6 YAL007C
12 4 1 5 YAR062W 2A6 YAR062W
13 1 1 7 YAL008W 1A7 YAL008W
14 2 1 7 YAR064W 2A7 YAR064W
15 3 1 7 Protein required for meiosis YAL009W 1A8 YAL009W
16 4 1 7 YAR066W 2A8 YAR066W
17 1 1 9 Mitochondrial outer membrane protein involved in mitochondrial morphology and inheritance YAL010C 1A9 YAL010C
18 2 1 9 YAR068W 2A9 YAR068W
19 3 1 9 YAL011W 1A10 YAL011W
20 4 1 9 YAR069C 2A10 YAR069C

Total number of rows: 6720

Table truncated, full table size 324 Kbytes.






Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary data files not provided

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