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Status
Public on Oct 05, 2010
Title
miRXplore TM Microarray (922)
Technology type
spotted oligonucleotide
Distribution
commercial
Organisms
Homo sapiens ; Mus musculus ; Rattus norvegicus Manufacturer
Miltenyi Biotec GmbH, Cologne, Germany
Manufacture protocol
1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted four times on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides using a customized piezoelectric spotter.
Description
The miRXplore TM Microarray is composed of 6228 DNA oligonucleotides with a sequence being reverse complement to the respective mature miRNA or control RNA, representing a total of 1460 miRNA sequences and 97 controls with all probes spotted in quadruplicates. The 1460 miRNA sequences covered sequence verified miRNAs of the species human (878) mouse (696) and rat (426) as well as viral miRNAs (141) and further mammalian species (76) as deposited in the miRBase sequence database version 10.1. The 97 positive and negative control probes were chosen as follows. Three sets of controls, miRControl 1 (four sequences used to assess efficiency and sensitivity of labelling), miRControl 2 (single sequence used to assess efficiency of small RNA enrichment) and miRControl 3 (43 sequences used to normalize signal intensities across different samples and arrays) were artificial sequences chosen not be present in any of the human, mouse, rat or viral genome. A fourth set of positive controls presented 36 positive small but non-miRNA sequences including probes e.g. for 5.1 sRNA, tRNAs, and U6 RNAs. A fifth set of oligonucleotides included 13 negative controls like different single or double mismatch, antisense, or scrambled miRNA sequences. Microarrays were manufactured by Miltenyi Biotec. Briefly, 1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides in quadruplicates using a customized piezoelectric spotter.
Web link
http://www.miltenyibiotec.com Contributor(s)
Gerstmayer B , Bosio A Submission date
Sep 29, 2010
Last update date
Mar 17, 2011
Contact name
Silvia Rueberg
E-mail(s)
[email protected] Organization name
Miltenyi Biotec GmbH
Department
Genomic Services
Street address
Friedrich-Ebert-Str. 68
City
Bergisch Gladbach
ZIP/Postal code
51429
Country
Germany
Samples (177)
GSM609843 , GSM609844 , GSM609845 , GSM694337 , GSM694338 , GSM694339
GSM720620 ,
GSM720621 ,
GSM720622 ,
GSM720623 ,
GSM763678 ,
GSM763679 ,
GSM763680 ,
GSM763681 ,
GSM763682 ,
GSM763683 ,
GSM763684 ,
GSM763685 ,
GSM763686 ,
GSM763687 ,
GSM763688 ,
GSM763689 ,
GSM763690 ,
GSM763691 ,
GSM763692 ,
GSM763693 ,
GSM763694 ,
GSM763695 ,
GSM763696 ,
GSM865746 ,
GSM865747 ,
GSM865748 ,
GSM865749 ,
GSM865750 ,
GSM865751 ,
GSM865752 ,
GSM865753 ,
GSM865754 ,
GSM865755 ,
GSM865756 ,
GSM865757 ,
GSM865758 ,
GSM865759 ,
GSM865760 ,
GSM865761 ,
GSM865762 ,
GSM865763 ,
GSM865764 ,
GSM865765 ,
GSM865766 ,
GSM865767 ,
GSM865768 ,
GSM865769 ,
GSM865770 ,
GSM865771 ,
GSM865772 ,
GSM865773 ,
GSM865774 ,
GSM865775 ,
GSM865776 ,
GSM865777 ,
GSM865778 ,
GSM865779 ,
GSM865780 ,
GSM865781 ,
GSM865782 ,
GSM865783 ,
GSM865818 ,
GSM865819 ,
GSM865820 ,
GSM865821 ,
GSM865822 ,
GSM865823 ,
GSM865824 ,
GSM865825 ,
GSM865826 ,
GSM865827 ,
GSM865828 ,
GSM865829 ,
GSM865830 ,
GSM865831 ,
GSM865832 ,
GSM865833 ,
GSM865835 ,
GSM865836 ,
GSM865837 ,
GSM865838 ,
GSM865839 ,
GSM865840 ,
GSM865841 ,
GSM865842 ,
GSM865843 ,
GSM865844 ,
GSM865845 ,
GSM865846 ,
GSM865847 ,
GSM865848 ,
GSM865849 ,
GSM865850 ,
GSM865851 ,
GSM865852 ,
GSM865853 ,
GSM865854 ,
GSM865855 ,
GSM865856 ,
GSM865857 ,
GSM865858 ,
GSM865859 ,
GSM865860 ,
GSM865861 ,
GSM865862 ,
GSM865863 ,
GSM865864 ,
GSM865865 ,
GSM865866 ,
GSM865867 ,
GSM865868 ,
GSM865869 ,
GSM865870 ,
GSM865871 ,
GSM865872 ,
GSM865873 ,
GSM865874 ,
GSM865875 ,
GSM865876 ,
GSM865877 ,
GSM865878 ,
GSM865879 ,
GSM865880 ,
GSM865881 ,
GSM865882 ,
GSM865883 ,
GSM865884 ,
GSM865885 ,
GSM865886 ,
GSM865887 ,
GSM865888 ,
GSM865889 ,
GSM865890 ,
GSM865891 ,
GSM865892 ,
GSM865893 ,
GSM865894 ,
GSM865895 ,
GSM865896 ,
GSM865897 ,
GSM865898 ,
GSM865899 ,
GSM865900 ,
GSM865901 ,
GSM865902 ,
GSM865903 ,
GSM865904 ,
GSM865905 ,
GSM865906 ,
GSM865907 ,
GSM865908 ,
GSM865909 ,
GSM865910 ,
GSM865911 ,
GSM865912 ,
GSM865913 ,
GSM865914 ,
GSM865915 ,
GSM865916 ,
GSM865917 ,
GSM865918 ,
GSM865919 ,
GSM865920 ,
GSM865921 ,
GSM865922 ,
GSM865923 ,
GSM865924 ,
GSM865925 ,
GSM865926 ,
GSM865927 ,
GSM865928 Series (4)
GSE24761 HeLa cell mitochondrial microRNAs
GSE29085 MicroRNA expression profiling of Ewing sarcoma cell lines
GSE30730 Argonaute-miRNA complexes reveal concerted action in disease related pathways of a human, glioblastoma cell line
GSE35320 microRNAs distinguish cytogenetic subgroups in pediatric AML and contribute to complex regulatory networks in AML-relevant pathways
Data table header descriptions ID stem-loop_acc Stem-loop_acc
mature_acc Mature_acc
name Name
miRNA_ID_LIST miRNA identifiers
miltenyi_id cDNA identifier at Miltenyi Biotec GmbH
SPOT_ID
Data table ID stem-loop_acc mature_acc name miRNA_ID_LIST miltenyi_id SPOT_ID 1
MI0000558 , MI0000829 , MI0000063 , MI0005453 , MI0005351 , MI0001172 MIMAT0000522 , MIMAT0000775 , MIMAT0000063 , MIMAT0004331 , MIMAT0004162 , MIMAT0001102 LET-7B LET-7B 35816
2
MI0000064 , MI0000559 , MI0000560 , MI0000830 , MI0000831 , MI0005454 , MI0002445 , MI0001174 MIMAT0000776 , MIMAT0000523 , MIMAT0000064 , MIMAT0004332 , MIMAT0002151 , MIMAT0001104 LET-7C LET-7C 35817
3
MI0000103 , MI0000739 , MI0000148 , MI0000886 , MI0002719 , MI0004735 , MI0002717 , MI0002723 , MI0002725 , MI0005283 , MI0005282 , MI0002720 , MI0002722 , MI0002721 , MI0002718 , MI0001270 , MI0007610 MIMAT0000133 , MIMAT0000099 , MIMAT0000823 , MIMAT0002427 , MIMAT0003520 , MIMAT0002425 , MIMAT0002431 , MIMAT0002433 , MIMAT0004098 , MIMAT0002428 , MIMAT0002430 , MIMAT0002429 , MIMAT0002426 , MIMAT0001185 MIR-101 MIR-101 35823
4
MI0000266 , MI0000685 , MI0000841 , MI0002791 , MI0005007 , MI0002788 , MI0005273 , MI0002792 , MI0002790 MIMAT0000782 , MIMAT0000648 , MIMAT0000253 , MIMAT0002489 , MIMAT0003786 , MIMAT0002486 , MIMAT0004089 , MIMAT0002490 , MIMAT0002488 MIR-10A MIR-10A 35824
5
MI0000442 , MI0000256 , MI0000891 , MI0005063 , MI0005287 , MI0002413 , MI0001277 , MI0001280 MIMAT0000827 , MIMAT0000246 , MIMAT0000421 , MIMAT0003849 , MIMAT0004101 , MIMAT0002119 , MIMAT0001190 MIR-122 MIR-122 35826
6
MI0000897 , MI0000446 , MI0000470 , MI0000152 , MI0000725 , MI0000896 , MI0002510 , MI0002575 , MI0004753 , MI0005457 , MI0002509 , MI0002574 , MI0002514 , MI0002579 , MI0002517 , MI0002581 , MI0005291 , MI0005292 , MI0002511 , MI0002513 , MI0002578 , MI0002512 , MI0002577 , MI MIMAT0000830 , MIMAT0000136 , MIMAT0000423 , MIMAT0002221 , MIMAT0003539 , MIMAT0002220 , MIMAT0002225 , MIMAT0002228 , MIMAT0004103 , MIMAT0002222 , MIMAT0002224 , MIMAT0002223 , MIMAT0002226 , MIMAT0002120 , MIMAT0001105 MIR-125B MIR-125B 35830
7
MI0000471 , MI0000153 , MI0000898 , MI0004754 , MI0001244 MIMAT0000137 , MIMAT0000444 , MIMAT0000831 , MIMAT0004328 , MIMAT0003723 MIR-126-5P MIR-126-5P 35832
8
MI0000473 , MI0000585 , MI0000637 MIMAT0000601 , MIMAT0000544 , MIMAT0004605 MIR-129-3P MIR-129-3P 35835
9
MI0000252 , MI0000473 , MI0000222 , MI0000585 , MI0000637 , MI0000902 , MI0005294 MIMAT0000600 , MIMAT0000209 , MIMAT0000242 , MIMAT0004105 MIR-129-5P MIR-129-5P 35836
10
MI0000448 , MI0000156 , MI0000903 , MI0002525 , MI0002524 , MI0002526 , MI0002527 MIMAT0000836 , MIMAT0000141 , MIMAT0000425 , MIMAT0002236 , MIMAT0002235 , MIMAT0002237 , MIMAT0002238 MIR-130A MIR-130A 35837
11
MI0000904 , MI0000748 , MI0000408 MIMAT0000837 , MIMAT0000387 , MIMAT0000691 MIR-130B MIR-130B 35838
12
MI0000474 , MI0000160 , MI0000907 , MI0002589 , MI0002591 , MI0002592 , MI0002590 MIMAT0000840 , MIMAT0000447 , MIMAT0000146 , MIMAT0002288 , MIMAT0002290 , MIMAT0002291 , MIMAT0002289 MIR-134 MIR-134 35841
13
MI0000475 , MI0000162 , MI0000909 , MI0002594 , MI0001523 , MI0002596 , MI0002593 , MI0002595 , MI0002452 MIMAT0000148 , MIMAT0000448 , MIMAT0000842 , MIMAT0002293 , MIMAT0001417 , MIMAT0002295 , MIMAT0002292 , MIMAT0002294 , MIMAT0002158 MIR-136 MIR-136 35843
14
MI0000454 , MI0000163 , MI0000910 , MI0005299 , MI0001221 MIMAT0000843 , MIMAT0000429 , MIMAT0000149 , MIMAT0004110 , MIMAT0001153 MIR-137 MIR-137 35844
15
MI0000456 , MI0000611 , MI0000165 , MI0002547 , MI0002437 , MI0001229 MIMAT0000573 , MIMAT0000431 , MIMAT0000151 , MIMAT0002255 , MIMAT0002143 , MIMAT0001159 MIR-140-5P MIR-140-5P 35847
16
MI0000457 , MI0000166 , MI0000914 , MI0002487 , MI0002485 , MI0002489 MIMAT0000846 , MIMAT0000153 , MIMAT0000432 , MIMAT0002198 , MIMAT0002196 , MIMAT0002200 MIR-141 MIR-141 35849
17
MI0000917 , MI0000460 , MI0000168 , MI0002556 , MI0002557 , MI0002554 , MI0002555 MIMAT0000156 , MIMAT0000436 , MIMAT0000850 , MIMAT0002264 , MIMAT0002265 , MIMAT0002262 , MIMAT0002263 MIR-144 MIR-144 35854
18
MI0000461 , MI0000169 , MI0000918 , MI0004756 , MI0002560 , MI0002558 , MI0002562 , MI0005305 , MI0002559 , MI0002561 , MI0002417 MIMAT0000851 , MIMAT0000157 , MIMAT0000437 , MIMAT0003542 , MIMAT0002268 , MIMAT0002266 , MIMAT0002270 , MIMAT0004116 , MIMAT0002267 , MIMAT0002269 , MIMAT0002123 MIR-145 MIR-145 35855
19
MI0000477 , MI0000170 , MI0000919 , MI0001235 MIMAT0000852 , MIMAT0000158 , MIMAT0000449 , MIMAT0001163 MIR-146A MIR-146A 35856
20
MI0000253 , MI0000550 , MI0004737 , MI0002418 , MI0001189 MIMAT0000516 , MIMAT0000243 , MIMAT0003522 , MIMAT0002124 , MIMAT0001120 MIR-148A MIR-148A 35857
1557 125 Kbytes .Supplementary data files not provided
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