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Status
Public on Jul 24, 2010
Title
Roseburia inulinivorans 5K array
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Roseburia inulinivorans Manufacturer
Rowett Institute of Nutrition and Health
Manufacture protocol
This array has been made from random genomic DNA fragments from R. inulinivorans A2-194. A total of 5500 individual colonies were picked from the library using a robotic colony picker and these were amplified by PCR using primers M13 forward and reverse. Approximately 5000 clones containing single inserts of 500 to 2500bp were identified and used for the array. Clones have been saved as glycerol stocks.
Support
glass
Coating
aminosilane
Description
The array has been printed in 48 blocks (4 columns and 12 rows), each block contains 324 features; 18 columns with 6 rows in triplicate at 0.20mm spacing. Mixed Arabidopsis thaliana cDNAs have been printed as landmarks in the top left corners of blocks 1,1; 1,4; 4,1; 4,4; 5,1; 5,4; 8,1; 8,4; 9,1; 9,4; 12,1 and 12,4.
Submission date
Feb 08, 2010
Last update date
Jul 24, 2010
Contact name
Gill Campbell
E-mail(s)
[email protected] Phone
+44 (0) 1224 716627
Fax
+44 (0) 1224 716647
URL
http://www.rowett.ac.uk Organization name
University of Aberdeen
Department
Rowett Institute of Nutrition and Health
Street address
Greenburn Road
City
Aberdeen
ZIP/Postal code
AB21 9SB
Country
United Kingdom
Samples (4)
GSM553706 , GSM553707 , GSM553708 , GSM553709 Series (1)
GSE22245 Differential gene expression in Roseburia inulinivorans grown on inulin and starch
Data table header descriptions ID MetaColumn Column number of the block within the array
MetaRow Row number of the block within the array
Column Spot column within the block
Row1 Spot row within the block for first replicate
Row2 Spot row within the block for second replicate
Row3 Spot row within the block for third replicate
BioSequence type Type of DNA sequence
Template Clone ID (culture plate and well position)
Reporter Identifier Clone ID (spot plate and well position)
Reporter usage Type of reporter
predicted function GB_ACC SPOT_ID
Data table ID MetaColumn MetaRow Column Row1 Row2 Row3 BioSequence type Template Reporter Identifier Reporter usage predicted function GB_ACC SPOT_ID 13I20
1
1
1
1
7
13
PCR_amplicon
relocated1
13I20
Landmark
mixed_A.thaliana_PCR_spikes
CONTROL
13A4
1
1
1
2
8
14
PCR_amplicon
13A4
13A4
Experimental
unknown
unknown
12I8
1
1
1
3
9
15
PCR_amplicon
9I8
12I8
Experimental
unknown
unknown
11A16
1
1
1
4
10
16
PCR_amplicon
11A16
11A16
Experimental
unknown
unknown
10I20
1
1
1
5
11
17
PCR_amplicon
10I20
10I20
Experimental
unknown
unknown
10A4
1
1
1
6
12
18
PCR_amplicon
10A4
10A4
Experimental
unknown
unknown
13M20
1
1
2
1
7
13
PCR_amplicon
13M20
13M20
Experimental
unknown
unknown
13E04
1
1
2
2
8
14
PCR_amplicon
13E04
13E04
Experimental
unknown
unknown
12M8
1
1
2
3
9
15
PCR_amplicon
9M8
12M8
Experimental
unknown
unknown
11E16
1
1
2
4
10
16
PCR_amplicon
11E16
11E16
Experimental
unknown
unknown
10M20
1
1
2
5
11
17
PCR_amplicon
10M20
10M20
Experimental
unknown
unknown
10E4
1
1
2
6
12
18
PCR_amplicon
10E4
10E4
Experimental
unknown
unknown
13A24
1
1
3
1
7
13
PCR_amplicon
13A24
13A24
Experimental
unknown
unknown
13I4
1
1
3
2
8
14
PCR_amplicon
13I4
13I4
Experimental
unknown
unknown
12A12
1
1
3
3
9
15
PCR_amplicon
9A12
12A12
Experimental
unknown
unknown
11I16
1
1
3
4
10
16
PCR_amplicon
11I16
11I16
Experimental
unknown
unknown
10A24
1
1
3
5
11
17
PCR_amplicon
10A24
10A24
Experimental
unknown
unknown
10I4
1
1
3
6
12
18
PCR_amplicon
10I4
10I4
Experimental
unknown
unknown
13E24
1
1
4
1
7
13
PCR_amplicon
3D22
13E24
Experimental
unknown
unknown
13M4
1
1
4
2
8
14
PCR_amplicon
13M4
13M4
Experimental
unknown
unknown
4992 352 Kbytes .Supplementary data files not provided
