Syt1 Synaptotagmin 1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Syt1provided by FlyBase
Official Full Name: Synaptotagmin 1provided by FlyBase
Primary source: FLYBASE:FBgn0004242
Locus tag: Dmel_CG3139
See related: AllianceGenome:FB:FBgn0004242
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 2L17; CG3139; D. Syt I; Dmel\CG3139; Droso1; DSypt; DSypt1; dSYT; DSyt; DSYT; dsyt1; dSyt1; DSyt1; DSYT1; Dsyt2; DSYT2; dsytI; l(2)23AB1; l(2)23Ba; l(2)k05909; STY1; syn; syt; Syt; SYT; syt 1; Syt 1; syt I; Syt I; SYT I; syt-1; Syt-1; syt1; SYT1; sytI; SytI
Summary: Enables several functions, including calcium ion binding activity; phospholipid binding activity; and protein homodimerization activity. Involved in several processes, including modulation of chemical synaptic transmission; secretion of lysosomal enzymes; and synaptic vesicle cycle. Located in neuromuscular junction; synaptic vesicle; and terminal bouton. Is expressed in several structures, including adult brain; adult somatic muscle cell; ganglia; neuromuscular junction; and presumptive embryonic/larval system. Used to study congenital myasthenic syndrome; congenital myasthenic syndrome 7; and neuromuscular junction disease. Human ortholog(s) of this gene implicated in congenital myasthenic syndrome and congenital myasthenic syndrome 7. Orthologous to several human genes including SYT2 (synaptotagmin 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 23A5-23B1; 2-7 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (2772327..2799979, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (2772327..2799979, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The role of the C2A domain of synaptotagmin 1 in asynchronous neurotransmitter release.
Title: The role of the C2A domain of synaptotagmin 1 in asynchronous neurotransmitter release.
This study uncovered essential residues within Synaptotagmin that suggest a structural basis for several activities required for fusion, including a C2B domain surface implicated in SNARE complex interaction that is required for rapid synchronization and Ca(2+) cooperativity of vesicle release.
Title: A synaptotagmin suppressor screen indicates SNARE binding controls the timing and Ca(2+) cooperativity of vesicle fusion.
tethering of Syt1 to synaptic vesicles in vivo is a prerequisite for its role in facilitating fast synchronous synaptic vesicle release and suppressing asynchronous and spontaneous fusion
Title: Transmembrane tethering of synaptotagmin to synaptic vesicles controls multiple modes of neurotransmitter release.
synaptic transmission can be regulated by Syt1 multimerization and that both C2 domains of Syt1 are uniquely required for modulating Ca(2+)-independent spontaneous fusion and Ca(2+)-dependent synchronous release.
Title: Genetic analysis of synaptotagmin C2 domain specificity in regulating spontaneous and evoked neurotransmitter release.
effect of APP gene on synaptotagmin 1 mRNA level
Title: Human APP gene expression in nerve cells of Drosophila melanogaster causes alteration of synaptoptagmin 1 mRNA level.
The major function of Ca2+ binding to synaptotagmin's C2A domain is to neutralize the negative charge of the pocket, thereby unleashing the fusion-stimulating activity of synaptotagmin.
Title: Calcium binding by synaptotagmin's C2A domain is an essential element of the electrostatic switch that triggers synchronous synaptic transmission.
this study provided direct support for the hypothesis that plasma membrane penetration, specifically by the C(2)B domain of synaptotagmin, is the critical effector interaction for coupling Ca(2+) binding with vesicle fusion
Title: Membrane penetration by synaptotagmin is required for coupling calcium binding to vesicle fusion in vivo.
Results suggest that the tandem C2 domains of Syt 1 play independent roles in neurotransmission.
Title: Differential regulation of synchronous versus asynchronous neurotransmitter release by the C2 domains of synaptotagmin 1.
Results demonstrate that synaptotagmin I plays a direct role in regulating spontaneous transmitter release, indicative of an active role in synaptic vesicle stabilization mediated by the C(2)A polylysine motif.
Title: Synaptotagmin I stabilizes synaptic vesicles via its C(2)A polylysine motif.
human APP gene plays role in synaptogenesis in transgenic lines of Drosophila melanogaster whose nerve cells express the human APP695 isoform, truncated APPs, and the presynaptic marker synaptotagmin driving the sequence of the green fluorescent protein.
Title: [Studying pathogenesis of Alzheimer's disease in a Drosophila melanogaster model: human APP overexpression in the brain of transgenic flies leads to deficit of the synaptic protein synaptotagmin].

Submit: New GeneRIF Correction See all GeneRIFs (20)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables SNARE binding	IBA Inferred from Biological aspect of Ancestor more info
enables SNARE binding	IPI Inferred from Physical Interaction more info	PubMed
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables calcium ion sensor activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-dependent phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-dependent phospholipid binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium-dependent phospholipid binding	TAS Traceable Author Statement more info	PubMed
enables phosphatidylserine binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in calcium ion-regulated exocytosis of neurotransmitter	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium-dependent activation of synaptic vesicle fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in clathrin-dependent synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in larval locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of liquid clearance, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter secretion	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of vesicle fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of calcium ion-dependent exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of neuromuscular synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of pole plasm oskar mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synapse structure or activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic vesicle exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in rhythmic synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in secretion of lysosomal enzymes	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle exocytosis	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
is_active_in dense core granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in exocytic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	ISM Inferred from Sequence Model more info
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	ISS Inferred from Sequence or Structural Similarity more info	PubMed
is_active_in synaptic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic vesicle membrane	NAS Non-traceable Author Statement more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: synaptotagmin 1

Names: CG3139-PA; CG3139-PB; CG3139-PC; CG3139-PE; CG3139-PF; CG3139-PG; CG3139-PH; CG3139-PI; Synaptotagmin-1; Synaptotagmin-I; Synaptotagmin1; SynaptotagminI; Syt I; Syt1-PA; Syt1-PB; Syt1-PC; Syt1-PE; Syt1-PF; Syt1-PG; Syt1-PH; Syt1-PI; synaptoptagmin 1; synaptotagmin; synaptotagmin I

NP_001285567.1

unusual splice

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

2772327..2799979 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001298639.1 → NP_001285568.1 synaptotagmin 1, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285568.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J4C1

Conserved Domains (3) summary

cd08385
Location:190 → 314

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:324 → 459

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1

pfam05808
Location:5 → 136

Podoplanin; Podoplanin
NM_164502.2 → NP_722839.1 synaptotagmin 1, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_722839.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J4C1

Related

FBpp0077412

Conserved Domains (3) summary

cd08385
Location:190 → 314

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:324 → 459

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1

pfam05808
Location:5 → 136

Podoplanin; Podoplanin
NM_205897.2 → NP_995619.2 synaptotagmin 1, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995619.2

Status: REVIEWED

UniProtKB/Swiss-Prot

P21521

Conserved Domains (3) summary

cd08385
Location:192 → 316

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:324 → 459

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1

pfam05808
Location:5 → 136

Podoplanin; Podoplanin
NM_164501.2 → NP_722838.1 synaptotagmin 1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_722838.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J4C1

Related

FBpp0077411

Conserved Domains (3) summary

cd08385
Location:190 → 314

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:324 → 459

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1

pfam05808
Location:5 → 136

Podoplanin; Podoplanin
NM_001298638.1 → NP_001285567.1 synaptotagmin 1, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285567.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J979

Conserved Domains (3) summary

cd08385
Location:192 → 316

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:325 → 460

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1

pfam05808
Location:5 → 136

Podoplanin; Podoplanin
NM_078736.3 → NP_523460.2 synaptotagmin 1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523460.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V023, M9PB17, M9PE99, P21521, Q7KU16, Q86NN2, Q8MRR8, Q9VQG7, Q9VQG8

Related

FBpp0077410

Conserved Domains (3) summary

cd08385
Location:192 → 316

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

cd08402
Location:326 → 461

C2B_Synaptotagmin-1; C2 domain second repeat present in Synaptotagmin 1

pfam05808
Location:5 → 136

Podoplanin; Podoplanin
NM_001370000.1 → NP_001356906.1 synaptotagmin 1, isoform G [Drosophila melanogaster]

Status: REVIEWED

Conserved Domains (3) summary

cd08385
Location:192 → 316

C2A_Synaptotagmin-1-5-6-9-10; C2A domain first repeat present in Synaptotagmins 1, 5, 6, 9, and 10

COG1766
Location:45 → 196

FliF; Flagellar biosynthesis/type III secretory pathway M-ring protein FliF/YscJ [Cell motility, Intracellular trafficking, secretion, and vesicular transport]

pfam12669
Location:120 → 148

P12; Virus attachment protein p12 family
NM_001273036.1 → NP_001259965.1 synaptotagmin 1, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259965.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P21521

Conserved Domains (1) summary

pfam12669
Location:120 → 151

P12; Virus attachment protein p12 family