cnn centrosomin [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: cnnprovided by FlyBase
Official Full Name: centrosominprovided by FlyBase
Primary source: FLYBASE:FBgn0013765
Locus tag: Dmel_CG4832
See related: AllianceGenome:FB:FBgn0013765
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: cen; CG 4832; CG18370; CG4832; Cnn; CNN; Cnn1; Dmel\CG4832; mat(2)syn-C; mat(2)synHK21; mfs; obv
Summary: Predicted to enable molecular adaptor activity. Involved in several processes, including embryonic cleavage; microtubule cytoskeleton organization; and nervous system development. Located in microtubule cytoskeleton. Is expressed in several structures, including brain; central brain primordium; epithelial furrow; gonad; and male germline cell. Human ortholog(s) of this gene implicated in Seckel syndrome; microcephaly; and primary autosomal recessive microcephaly 3. Orthologous to several human genes including CDK5RAP2 (CDK5 regulatory subunit associated protein 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 50A8-50A9; 2-68 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (13439314..13450475, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (9326819..9337980, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Multifaceted modes of gamma-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.
Title: Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.
Autoinhibition of Cnn binding to gamma-TuRCs prevents ectopic microtubule nucleation and cell division defects.
Title: Autoinhibition of Cnn binding to γ-TuRCs prevents ectopic microtubule nucleation and cell division defects.
These findings suggest that interactions between Spd-2, Polo and Cnn form a positive feedback loop that drives the dramatic expansion of the mitotic pericentriolar material in fly embryos.
Title: Evidence that a positive feedback loop drives centrosome maturation in fly embryos.
Drosophila centrosomin (cnn) expresses two major protein variants: the centrosomal form (CnnC) and a non-centrosomal form in testes (CnnT). CnnC is established as essential for functional centrosomes, the major MTOCs in animal cells. CnnT is expressed exclusively in testes and localizes to giant mitochondria in spermatids.
Title: A Splice Variant of Centrosomin Converts Mitochondria to Microtubule-Organizing Centers.
suggest an expanded model where during interphase CM2 interacts with pericentrin-like-protein to form a layer of centrosomin around the centriole wall and that at the onset of mitosis this population acts as a nucleation site of intramolecular centrosomin interactions that support the expansion into the metaphase matrix
Title: The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles.
Study shows that cnn exon1A-encoded proteins interact with Polo at 2 residues and that this interaction is required for polar body and pericentriolar matrix formation in syncytial embryos.
Title: The end of a monolith: Deconstructing the Cnn-Polo interaction.
During pericentriolar matrix formation exon 1A Cnn-Long Form proteins likely bind Polo kinase before phosphorylation by Polo for Cnn transport to the centrosome.
Title: An Amino-Terminal Polo Kinase Interaction Motif Acts in the Regulation of Centrosome Formation and Reveals a Novel Function for centrosomin (cnn) in Drosophila.
The findings reveal that polarized targeting of Centrosomin to Golgi outposts during elaboration of the dendrite arbor creates a local system for guiding microtubule polymerization.
Title: Centrosomin represses dendrite branching by orienting microtubule nucleation.
Cnn and PLP directly interact at two defined sites to coordinate the cell cycle-dependent rearrangement and scaffolding activity of the centrosome to permit normal centrosome organization, cell division, and embryonic viability.
Title: Interphase centrosome organization by the PLP-Cnn scaffold is required for centrosome function.
Data indicate that interphase centrioles are closely associated with Sas-4, Spd-2, Polo kinase, Pericentrin-like protein (Dplp), Asterless (Asl), Plk4 kinase, Centrosomin (Cnn) and gamma-tubulin.
Title: Structured illumination of the interface between centriole and peri-centriolar material.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables molecular adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in asymmetric cell division	IDA Inferred from Direct Assay more info	PubMed
involved_in asymmetric neuroblast division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in central nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centriole replication	IDA Inferred from Direct Assay more info	PubMed
involved_in centrosome cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in centrosome cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic cleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in midgut development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peripheral nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in photoreceptor cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole cell formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of Golgi organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of centriole-centriole cohesion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in centriole	IDA Inferred from Direct Assay more info	PubMed
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in ciliary basal body	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule organizing center	IEA Inferred from Electronic Annotation more info
located_in pericentriolar material	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: centrosomin

Names: CDK5RAP2; CG4832-PA; CG4832-PB; CG4832-PD; CG4832-PE; CG4832-PG; CG4832-PH; CG4832-PI; CG4832-PJ; CG4832-PK; CG4832-PL; CG4832-PM; CG4832-PN; centrosmin; cnn-PA; cnn-PB; cnn-PD; cnn-PE; cnn-PG; cnn-PH; cnn-PI; cnn-PJ; cnn-PK; cnn-PL; cnn-PM; cnn-PN; maternal effect syncytial blastoderm arrest C; oblivious

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

13439314..13450475 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057736.6 → NP_477084.1 centrosomin, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477084.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P54623

Related

FBpp0086822

Conserved Domains (3) summary

COG1196
Location:192 → 973

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

pfam07989
Location:101 → 170

Cnn_1N; Centrosomin N-terminal motif 1

pfam16046
Location:789 → 857

FAM76; FAM76 protein
NM_001259364.2 → NP_001246293.1 centrosomin, isoform L [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246293.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K863

Conserved Domains (3) summary

pfam07989
Location:101 → 170

Cnn_1N; Centrosomin N-terminal motif 1

pfam15905
Location:891 → 1203

HMMR_N; Hyaluronan mediated motility receptor N-terminal

pfam16046
Location:1019 → 1087

FAM76; FAM76 protein
NM_001259366.2 → NP_001246295.1 centrosomin, isoform N [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246295.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7N9

Conserved Domains (3) summary

pfam07989
Location:73 → 142

Cnn_1N; Centrosomin N-terminal motif 1

pfam15905
Location:633 → 945

HMMR_N; Hyaluronan mediated motility receptor N-terminal

pfam16046
Location:761 → 829

FAM76; FAM76 protein
NM_001259365.2 → NP_001246294.1 centrosomin, isoform M [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246294.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7N9

Conserved Domains (3) summary

pfam07989
Location:73 → 142

Cnn_1N; Centrosomin N-terminal motif 1

pfam15905
Location:633 → 945

HMMR_N; Hyaluronan mediated motility receptor N-terminal

pfam16046
Location:761 → 829

FAM76; FAM76 protein
NM_165990.3 → NP_725297.1 centrosomin, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725297.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7N9

Related

FBpp0086823

Conserved Domains (3) summary

pfam07989
Location:73 → 142

Cnn_1N; Centrosomin N-terminal motif 1

pfam15905
Location:633 → 945

HMMR_N; Hyaluronan mediated motility receptor N-terminal

pfam16046
Location:761 → 829

FAM76; FAM76 protein
NM_001038855.3 → NP_001033944.1 centrosomin, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001033944.1

Status: REVIEWED

UniProtKB/TrEMBL

A1Z9D3

Conserved Domains (3) summary

pfam07989
Location:73 → 142

Cnn_1N; Centrosomin N-terminal motif 1

pfam15905
Location:643 → 955

HMMR_N; Hyaluronan mediated motility receptor N-terminal

pfam16046
Location:771 → 839

FAM76; FAM76 protein
NM_001014523.2 → NP_001014523.1 centrosomin, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001014523.1

Status: REVIEWED

UniProtKB/TrEMBL

A1Z9D4

Conserved Domains (3) summary

COG1196
Location:134 → 915

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

pfam07989
Location:43 → 112

Cnn_1N; Centrosomin N-terminal motif 1

pfam16046
Location:731 → 799

FAM76; FAM76 protein
NM_165991.4 → NP_725298.1 centrosomin, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725298.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A0A0B4K705, E1JH56, P54623, Q5BIF5, Q86NP3, Q8IHE5, Q8ML65, Q9V6P7, Q9V6P8

Related

FBpp0301623

Conserved Domains (3) summary

pfam07989
Location:43 → 112

Cnn_1N; Centrosomin N-terminal motif 1

pfam15905
Location:833 → 1145

HMMR_N; Hyaluronan mediated motility receptor N-terminal

pfam16046
Location:961 → 1029

FAM76; FAM76 protein
NM_001259363.2 → NP_001246292.1 centrosomin, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246292.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K773

Conserved Domains (2) summary

pfam07989
Location:101 → 170

Cnn_1N; Centrosomin N-terminal motif 1

pfam13863
Location:213 → 318

DUF4200; Domain of unknown function (DUF4200)
NM_001259367.1 → NP_001246296.1 centrosomin, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246296.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K705

Conserved Domains (2) summary

pfam07989
Location:43 → 112

Cnn_1N; Centrosomin N-terminal motif 1

pfam13863
Location:155 → 260

DUF4200; Domain of unknown function (DUF4200)
NM_001169670.2 → NP_001163141.1 centrosomin, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163141.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P54623

UniProtKB/TrEMBL

E1JH56

Conserved Domains (2) summary

pfam07989
Location:43 → 112

Cnn_1N; Centrosomin N-terminal motif 1

pfam13863
Location:155 → 260

DUF4200; Domain of unknown function (DUF4200)
NM_001259362.1 → NP_001246291.1 centrosomin, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246291.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K6Z9

Conserved Domains (2) summary

pfam07989
Location:43 → 112

Cnn_1N; Centrosomin N-terminal motif 1

pfam13863
Location:155 → 260

DUF4200; Domain of unknown function (DUF4200)

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
A0A0B4K6Z9		A0A0B4K6Z9
A0A0B4K773		A0A0B4K773
A0A0B4K7N9		A0A0B4K7N9
A0A0B4K863		A0A0B4K863
A1Z9D3		A1Z9D3
A1Z9D4		A1Z9D4
P54623.3		P54623