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    PRKAA1 protein kinase AMP-activated catalytic subunit alpha 1 [ Homo sapiens (human) ]

    Gene ID: 5562, updated on 4-Jan-2025

    Summary

    Official Symbol
    PRKAA1provided by HGNC
    Official Full Name
    protein kinase AMP-activated catalytic subunit alpha 1provided by HGNC
    Primary source
    HGNC:HGNC:9376
    See related
    Ensembl:ENSG00000132356 MIM:602739; AllianceGenome:HGNC:9376
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    AMPK; AMPKa1; AMPK alpha 1
    Summary
    The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in gall bladder (RPKM 18.4), thyroid (RPKM 16.3) and 25 other tissues See more
    Orthologs
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    Genomic context

    See PRKAA1 in Genome Data Viewer
    Location:
    5p13.1
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 5 NC_000005.10 (40759389..40798374, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 5 NC_060929.1 (41015789..41054877, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (40759491..40798476, complement)

    Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene small nucleolar RNA SNORA63 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 15982 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr5:40681035-40681562 Neighboring gene prostaglandin E receptor 4 Neighboring gene tetratricopeptide repeat domain 33 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 22503 Neighboring gene uncharacterized LOC124900968 Neighboring gene HNF1 motif-containing MPRA enhancer 12 Neighboring gene small nucleolar RNA SNORA57 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 15983 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 15984 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr5:40801183-40802004 Neighboring gene CRISPRi-validated cis-regulatory element chr5.1161 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 22504 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr5:40835903-40836726 Neighboring gene ribosomal protein L37 Neighboring gene small nucleolar RNA, C/D box 72 Neighboring gene caspase recruitment domain family member 6

    Genomic regions, transcripts, and products

    Expression

    • Project title: Tissue-specific circular RNA induction during human fetal development
    • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
    • BioProject: PRJNA270632
    • Publication: PMID 26076956
    • Analysis date: Mon Apr 2 22:54:59 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    BioGRID CRISPR Screen Phenotypes (31 hits/1303 screens)

    EBI GWAS Catalog

    Description
    A genome-wide association study identifies new susceptibility loci for non-cardia gastric cancer at 3q13.31 and 5p13.1.
    EBI GWAS Catalog

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat HIV-1 Tat-induced LTR transactivation is reversed by tanshinone II A through the AMPK-Nampt pathway PubMed
    tat MiR-217 regulates HIV-1 Tat-induced activation of NF-kappaB and inactivation of AMPK in MAGI cells PubMed
    tat Knockdown of SIRT1 by its inhibitor or siRNA results in Tat-mediated reduction of phosphorylation of AMP-activated protein kinase (AMPK) and downstream acetyl-CoA carboxylase (ACC) PubMed
    tat Knockdown of PRKAA1 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed
    Vif vif Protein kinase A (PKA) binds and phosphorylates A3G at Thr32 in vitro and in vivo. This phosphorylation event reduces the binding of A3G to Vif and its subsequent ubiquitination and degradation PubMed
    Vpr vpr HIV-1 Vpr directly interacts with PKA and is phosphorylated at position Ser79 by PKA. PKA activity is necessary for virion-delivered Vpr cell cycle arrest PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    General gene information

    Clone Names

    • MGC33776, MGC57364

    Gene Ontology Provided by GOA

    Items 1 - 25 of 35
    Function Evidence Code Pubs
    enables 3-phosphoinositide-dependent protein kinase activity  
    enables AMP-activated protein kinase activity PubMed 
    enables AMP-activated protein kinase activity  
    enables AMP-activated protein kinase activity PubMed 
    enables ATP binding  
    enables DNA-dependent protein kinase activity  
    enables Rho-dependent protein serine/threonine kinase activity  
    enables [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity  
    enables cAMP-dependent protein kinase activity PubMed 
    enables chromatin binding  
    enables eukaryotic translation initiation factor 2alpha kinase activity  
    enables histone H2AS1 kinase activity  
    enables histone H2AS121 kinase activity  
    enables histone H2AT120 kinase activity  
    enables histone H2AXS139 kinase activity  
    enables histone H2BS14 kinase activity  
    enables histone H2BS36 kinase activity  
    enables histone H3S10 kinase activity  
    enables histone H3S28 kinase activity  
    enables histone H3S57 kinase activity  
    enables histone H3T11 kinase activity  
    enables histone H3T3 kinase activity  
    enables histone H3T45 kinase activity  
    enables histone H3T6 kinase activity  
    enables histone H4S1 kinase activity  
    enables metal ion binding  
    enables protein binding PubMed 
    enables protein kinase activity PubMed 
    enables protein serine kinase activity  
    enables protein serine/threonine kinase activity  
    enables protein serine/threonine kinase activity PubMed 
    enables protein-containing complex binding  
    enables ribosomal protein S6 kinase activity  
    enables tau protein binding PubMed 
    enables tau-protein kinase activity PubMed 
    Items 1 - 25 of 35
    Items 1 - 25 of 72
    Process Evidence Code Pubs
    involved_in CAMKK-AMPK signaling cascade PubMed 
    involved_in CAMKK-AMPK signaling cascade PubMed 
    involved_in CAMKK-AMPK signaling cascade PubMed 
    involved_in Wnt signaling pathway  
    involved_in autophagy  
    involved_in cellular response to calcium ion  
    involved_in cellular response to ethanol  
    involved_in cellular response to glucose starvation  
    involved_in cellular response to glucose starvation PubMed 
    involved_in cellular response to glucose stimulus  
    involved_in cellular response to hydrogen peroxide  
    involved_in cellular response to hypoxia  
    involved_in cellular response to nutrient levels PubMed 
    involved_in cellular response to nutrient levels  
    involved_in cellular response to oxidative stress  
    involved_in cellular response to prostaglandin E stimulus  
    involved_in cellular response to stress PubMed 
    involved_in cellular response to xenobiotic stimulus  
    involved_in cholesterol biosynthetic process  
    involved_in chromatin remodeling  
    involved_in cold acclimation  
    involved_in energy homeostasis  
    involved_in energy homeostasis PubMed 
    involved_in fatty acid biosynthetic process  
    involved_in fatty acid homeostasis  
    involved_in fatty acid oxidation  
    involved_in glucose homeostasis  
    involved_in glucose metabolic process  
    involved_in lipid biosynthetic process  
    acts_upstream_of_positive_effect lipid droplet disassembly PubMed 
    involved_in motor behavior PubMed 
    involved_in negative regulation of TOR signaling  
    involved_in negative regulation of TOR signaling  
    involved_in negative regulation of TORC1 signaling PubMed 
    involved_in negative regulation of apoptotic process  
    involved_in negative regulation of gene expression  
    involved_in negative regulation of glucosylceramide biosynthetic process PubMed 
    involved_in negative regulation of hepatocyte apoptotic process PubMed 
    involved_in negative regulation of insulin receptor signaling pathway  
    involved_in negative regulation of lipid catabolic process  
    involved_in negative regulation of tubulin deacetylation  
    involved_in neuron cellular homeostasis PubMed 
    involved_in positive regulation of DNA-templated transcription  
    involved_in positive regulation of T cell activation PubMed 
    involved_in positive regulation of T cell mediated immune response to tumor cell PubMed 
    involved_in positive regulation of adipose tissue development  
    involved_in positive regulation of autophagy PubMed 
    involved_in positive regulation of autophagy PubMed 
    involved_in positive regulation of autophagy  
    involved_in positive regulation of cell population proliferation  
    involved_in positive regulation of cholesterol biosynthetic process PubMed 
    involved_in positive regulation of gene expression  
    involved_in positive regulation of glycolytic process  
    involved_in positive regulation of mitochondrial transcription  
    involved_in positive regulation of peptidyl-lysine acetylation  
    involved_in positive regulation of protein localization  
    involved_in positive regulation of protein targeting to mitochondrion  
    involved_in positive regulation of skeletal muscle tissue development  
    involved_in protein localization to lipid droplet PubMed 
    involved_in protein phosphorylation PubMed 
    involved_in regulation of circadian rhythm  
    involved_in regulation of microtubule cytoskeleton organization  
    involved_in regulation of stress granule assembly  
    involved_in regulation of vesicle-mediated transport  
    involved_in response to UV  
    involved_in response to activity  
    involved_in response to caffeine  
    involved_in response to estrogen  
    involved_in response to gamma radiation  
    involved_in response to hypoxia PubMed 
    involved_in rhythmic process  
    involved_in signal transduction PubMed 
    Items 1 - 25 of 72
    Component Evidence Code Pubs
    located_in apical plasma membrane  
    located_in axon  
    located_in chromatin  
    is_active_in cytoplasm  
    is_active_in cytoplasm PubMed 
    located_in cytoplasm PubMed 
    located_in cytosol  
    located_in cytosol  
    located_in dendrite  
    located_in neuronal cell body  
    located_in nuclear speck  
    located_in nucleoplasm  
    part_of nucleotide-activated protein kinase complex  
    part_of nucleotide-activated protein kinase complex PubMed 
    part_of nucleotide-activated protein kinase complex  
    part_of nucleotide-activated protein kinase complex PubMed 
    is_active_in nucleus  
    located_in nucleus  

    General protein information

    Preferred Names
    5'-AMP-activated protein kinase catalytic subunit alpha-1
    Names
    5'-AMP-activated protein kinase, catalytic alpha-1 chain
    ACACA kinase
    AMP -activate kinase alpha 1 subunit
    AMP-activated protein kinase, catalytic, alpha-1
    AMPK subunit alpha-1
    AMPK, alpha, 1
    HMGCR kinase
    acetyl-CoA carboxylase kinase
    hydroxymethylglutaryl-CoA reductase kinase
    protein kinase, AMP-activated, alpha 1 catalytic subunit
    tau-protein kinase PRKAA1
    NP_001341957.1
    NP_001341958.1
    NP_001341963.1
    NP_001341964.1
    NP_001341965.1
    NP_001341966.1
    NP_006242.5
    NP_996790.3
    XP_016865113.1
    XP_054208885.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001355028.2 → NP_001341957.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 3

      Status: REVIEWED

      Source sequence(s)
      AC008810
      Conserved Domains (3) summary
      cd12199
      Location:378 → 531
      AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit
      cd14403
      Location:270 → 334
      UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)
      cd14079
      Location:17 → 253
      STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase
    2. NM_001355029.2 → NP_001341958.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 4

      Status: REVIEWED

      Source sequence(s)
      AC008810
      Conserved Domains (3) summary
      cd12199
      Location:260 → 413
      AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit
      cd14403
      Location:152 → 216
      UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)
      cl21453
      Location:1 → 135
      PKc_like; Protein Kinases, catalytic domain
    3. NM_001355034.2 → NP_001341963.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 5

      Status: REVIEWED

      Source sequence(s)
      AC008810
      UniProtKB/TrEMBL
      Q96E92
      Related
      ENSP00000296800.4, ENST00000296800.4
      Conserved Domains (1) summary
      cl21453
      Location:24 → 199
      PKc_like; Protein Kinases, catalytic domain
    4. NM_001355035.2 → NP_001341964.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 6

      Status: REVIEWED

      Description
      Transcript Variant: This variant (6), as well as variants 7 and 8, encodes isoform 6.
      Source sequence(s)
      AC008810
      Conserved Domains (3) summary
      cd12199
      Location:146 → 299
      AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit
      cd14403
      Location:38 → 102
      UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)
      cl21453
      Location:1 → 21
      PKc_like; Protein Kinases, catalytic domain
    5. NM_001355036.2 → NP_001341965.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 6

      Status: REVIEWED

      Description
      Transcript Variant: This variant (7), as well as variants 6 and 8, encodes isoform 6.
      Source sequence(s)
      AC008810
      Conserved Domains (3) summary
      cd12199
      Location:146 → 299
      AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit
      cd14403
      Location:38 → 102
      UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)
      cl21453
      Location:1 → 21
      PKc_like; Protein Kinases, catalytic domain
    6. NM_001355037.2 → NP_001341966.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 6

      Status: REVIEWED

      Description
      Transcript Variant: This variant (8), as well as variants 6 and 7, encodes isoform 6.
      Source sequence(s)
      AC008810
      Conserved Domains (3) summary
      cd12199
      Location:146 → 299
      AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit
      cd14403
      Location:38 → 102
      UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)
      cl21453
      Location:1 → 21
      PKc_like; Protein Kinases, catalytic domain
    7. NM_006251.6 → NP_006242.5  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1

      See identical proteins and their annotated locations for NP_006242.5

      Status: REVIEWED

      Source sequence(s)
      AC008810
      Consensus CDS
      CCDS3932.2
      UniProtKB/Swiss-Prot
      A8MTQ6, B2R7E1, O00286, Q13131, Q5D0E1, Q86VS1, Q9UNQ4
      Related
      ENSP00000380317.2, ENST00000397128.7
      Conserved Domains (3) summary
      cd12199
      Location:404 → 557
      AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit
      cd14403
      Location:296 → 360
      UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)
      cd14079
      Location:24 → 279
      STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase
    8. NM_206907.4 → NP_996790.3  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 2

      See identical proteins and their annotated locations for NP_996790.3

      Status: REVIEWED

      Source sequence(s)
      AC008810
      Consensus CDS
      CCDS3933.2
      UniProtKB/Swiss-Prot
      Q13131
      Related
      ENSP00000346148.3, ENST00000354209.7
      Conserved Domains (3) summary
      cd12199
      Location:419 → 572
      AMPKA1_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit
      cd14403
      Location:311 → 375
      UBA_AID_AAPK1; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPKalpha-1)
      cd14079
      Location:24 → 294
      STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000005.10 Reference GRCh38.p14 Primary Assembly

      Range
      40759389..40798374 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017009624.2 → XP_016865113.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060929.1 Alternate T2T-CHM13v2.0

      Range
      41015789..41054877 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054352910.1 → XP_054208885.1  5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1

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