rad-51 DNA repair protein RAD51 homolog [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: rad-51
Official Full Name: DNA repair protein RAD51 homolog
Primary source: WormBase:WBGene00004297
Locus tag: CELE_Y43C5A.6
See related: AllianceGenome:WB:WBGene00004297
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables several functions, including ATP hydrolysis activity; DEAD/H-box RNA helicase binding activity; and catalytic activity, acting on DNA. Involved in several processes, including intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; meiotic chromosome condensation; and negative regulation of apoptotic process. Located in condensed nuclear chromosome; germ cell nucleus; and site of double-strand break. Is expressed in gonad. Human ortholog(s) of this gene implicated in Fanconi anemia complementation group R; breast cancer; congenital mirror movement disorder; and ovarian cancer. Orthologous to human RAD51 (RAD51 recombinase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See rad-51 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 8

Sequence:: Chromosome: IV; NC_003282.8 (10281634..10284244, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins.
Title: Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins.
Data show that replication factor A (RPA-1) and DNA repair protein RAD51 (RAD-51) colocalization for hours post-microirradiation throughout the germline.
Title: Differential RPA-1 and RAD-51 recruitment in vivo throughout the C. elegans germline, as revealed by laser microirradiation.
Study suggests that the rad-51 gene has a key role in the induction of germ line apoptosis downstream of the DNA damage checkpoint, and that this is not mediated through alteration of the apoptotic machinery.
Title: A Role in Apoptosis Regulation for the rad-51 Gene of Caenorhabditis elegans.
Analyses of jmjd-5 genetic interactions with genes required for resolving recombination intermediates (rtel-1) or promoting the resolution of RAD-51 double stranded DNA filaments (rfs-1 and helq-1) suggest that jmjd-5 prevents the formation of stalled postsynaptic recombination intermediates and favors RAD-51 removal.
Title: JMJD-5/KDM8 regulates H3K36me2 and is required for late steps of homologous recombination and genome integrity.
Rad51 paralog complex, RFS-1/RIP-1, from Caenorhabditis elegans is required for homologous recombination and RAD-51 focus formation at DNA damage sites in vivo, and it stimulates the recombinase activity of RAD-51 and associates directly with RAD-51 filaments in vitro.
Title: Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination.
When CeBRC-2 was introduced into BRCA2-deficient cells, it restored Rad51 foci after DNA damage. Our study suggests a mode of action for BRCA2 with regard to DNA repair.
Title: Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis.
Completely suppresses massive increase in the level of double-strand breaks caused by depletion of him-6 and top-3.
Title: Multiple genetic pathways involving the Caenorhabditis elegans Bloom's syndrome genes him-6, rad-51, and top-3 are needed to maintain genome stability in the germ line.
One hypothesis is that SCE is a manifestation of Rad51-dependent homologous recombination repair.
Title: Rad51C-deficient CL-V4B cells exhibit normal levels of mitomycin C-induced SCEs but reduced levels of UVC-induced SCEs.
These results support a model where an interaction with RAD-51 alone is likely involved in filament nucleation, whereas a second independent interaction is involved in stabilization of RAD-51 filaments by BRCA2.
Title: Stabilization of RAD-51-DNA filaments via an interaction domain in Caenorhabditis elegans BRCA2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y43C5A.6 (e-PCR)
- UniSTS:313310

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent activity, acting on DNA	IEA Inferred from Electronic Annotation more info
enables DEAD/H-box RNA helicase binding	IPI Inferred from Physical Interaction more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA strand exchange activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA strand exchange activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA strand exchange activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombinase assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA strand invasion	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome organization involved in meiotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	IEA Inferred from Electronic Annotation more info
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic recombination-dependent replication fork processing	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in condensed nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA repair protein RAD51 homolog

NP_001023465.1

Confirmed by transcript evidence

NP_001263771.1

Confirmed by transcript evidence

NP_001379410.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

10281634..10284244 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001028294.5 → NP_001023465.1 DNA repair protein RAD51 homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023465.1

Status: REVIEWED

UniProtKB/TrEMBL

Q95Q25

Conserved Domains (3) summary

PTZ00035
Location:62 → 394

PTZ00035; Rad51 protein; Provisional

cd01123
Location:156 → 390

Rad51_DMC1_radA; Rad51_DMC1_radA,B. This group of recombinases includes the eukaryotic proteins RAD51, RAD55/57 and the meiosis-specific protein DMC1, and the archaeal proteins radA and radB. They are closely related to the bacterial RecA group. Rad51 proteins catalyze a ...

pfam14520
Location:94 → 132

HHH_5; Helix-hairpin-helix domain
NM_001392385.1 → NP_001379410.1 DNA repair protein RAD51 homolog [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

G5EGG8

Conserved Domains (1) summary

PTZ00035
Location:24 → 356

PTZ00035; Rad51 protein; Provisional
NM_001276842.3 → NP_001263771.1 DNA repair protein RAD51 homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001263771.1

Status: REVIEWED

UniProtKB/TrEMBL

L8E822

Conserved Domains (3) summary

PTZ00035
Location:29 → 361

PTZ00035; Rad51 protein; Provisional

cd01123
Location:123 → 357

Rad51_DMC1_radA; Rad51_DMC1_radA,B. This group of recombinases includes the eukaryotic proteins RAD51, RAD55/57 and the meiosis-specific protein DMC1, and the archaeal proteins radA and radB. They are closely related to the bacterial RecA group. Rad51 proteins catalyze a ...

pfam14520
Location:61 → 99

HHH_5; Helix-hairpin-helix domain