Apc APC-like [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Apcprovided by FlyBase
Official Full Name: APC-likeprovided by FlyBase
Primary source: FLYBASE:FBgn0015589
Locus tag: Dmel_CG1451
See related: AllianceGenome:FB:FBgn0015589
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: apc; APC; apc 1; apc1; Apc1; APC1; CG1451; d-APC; D-APC; D-APC1; dApc; dAPC; DAPC; dapc1; dAPC1; Dm APC1; Dmel\CG1451
Summary: Enables beta-catenin binding activity. Involved in several processes, including nervous system development; oogenesis; and regionalization. Located in apical part of cell; axon; and cytosol. Part of beta-catenin destruction complex. Is expressed in several structures, including epidermis; motor neurons; neuron projection bundle of larval ventral nerve cord; presumptive embryonic/larval nervous system; and tendon cell. Used to study colorectal cancer and familial adenomatous polyposis 1. Human ortholog(s) of this gene implicated in several diseases, including Sotos syndrome 3; autosomal recessive intellectual developmental disorder 74; carcinoma (multiple); gastrointestinal system cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human APC (APC regulator of WNT signaling pathway) and APC2 (APC regulator of WNT signaling pathway 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 98E5-98E6; 3-97 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (28832301..28844748, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (24658023..24670470, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic beta-catenin.
Title: Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic β-catenin.
Novel requirement for APC in Axin regulation through phosphorylation both prevents signaling in the Wnt-off state and promotes signaling immediately following Wnt stimulation.
Title: Axin phosphorylation in both Wnt-off and Wnt-on states requires the tumor suppressor APC.
in the adult intestine, Ebd acts independently of Ewg in physiological Wnt signaling, but cooperates with Ewg to induce the hyperactivation of Wnt target gene expression following Apc1 loss. These findings have relevance for human tumorigenesis, as Jerky (JRK/JH8), the human Ebd homolog, promotes Wnt pathway hyperactivation and is overexpressed in colorectal, breast, and ovarian cancers
Title: Intestinal stem cell overproliferation resulting from inactivation of the APC tumor suppressor requires the transcription cofactors Earthbound and Erect wing.
We conclude that even in the unstimulated state, a pool of Axin forms membrane-proximal puncta that are dependent on Apc, and that membrane association regulates both Axin levels and Axin's role in the rapid activation of signaling that follows Wnt exposure.
Title: Dual Roles for Membrane Association of Drosophila Axin in Wnt Signaling.
Data indicate that Ras is not required for Wnt signaling activation after APC loss.
Title: APC loss-induced intestinal tumorigenesis in Drosophila: Roles of Ras in Wnt signaling activation and tumor progression.
Drosophila and vertebrate APCs exhibit highly related activities and mechanisms and directly collaborate with formins such as diaphanous.
Title: Drosophila homologues of adenomatous polyposis coli (APC) and the formin diaphanous collaborate by a conserved mechanism to stimulate actin filament assembly.
co-expression experiments reveal that APC displaces Dishevelled from Axin assemblies, promoting degradasome over signalosome formation in the absence of Wnts
Title: The Adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled.
Non-autonomous crosstalk between the Jak/Stat and Egfr pathways mediates Apc1-driven intestinal stem cell hyperplasia in the Drosophila adult midgut.
Title: Non-autonomous crosstalk between the Jak/Stat and Egfr pathways mediates Apc1-driven intestinal stem cell hyperplasia in the Drosophila adult midgut.
Data show that a loss of function for dapc1 or both dapc1 and dapc2 (dapc) in ovary border (BC) and adjacent follicle cells impaired BC delamination, and this mutant phenotype can be rescued upon overexpression of either dapc1 or dapc2.
Title: Drosophila apc regulates delamination of invasive epithelial clusters.
spindle assembly checkpoint is not responsible for the inability of APC(Fzy) to target cyclin B and promote anaphase in metaphase II arrested eggs from cort mutant females
Title: Evidence that the spindle assembly checkpoint does not regulate APC(Fzy) activity in Drosophila female meiosis.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables beta-catenin binding	IBA Inferred from Biological aspect of Ancestor more info
enables beta-catenin binding	IDA Inferred from Direct Assay more info	PubMed
enables beta-catenin binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in brain development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell fate specification	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in chitin-based larval cuticle pattern formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in imaginal disc pattern formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of canonical Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of canonical Wnt signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of compound eye retinal cell programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of microtubule depolymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in neuroblast proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in oocyte localization involved in germarium-derived egg chamber formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in pattern specification process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of axo-dendritic protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in segment polarity determination	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
part_of beta-catenin destruction complex	IBA Inferred from Biological aspect of Ancestor more info
part_of beta-catenin destruction complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of catenin complex	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cytoplasmic microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: APC-like

Names: Apc-PA; Apc-PB; Apc-PC; CG1451-PA; CG1451-PB; CG1451-PC; adenomatous polyposis coli; adenomatous polyposis coli 1; adenomatous polyposis coli protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

28832301..28844748 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057804.3 → NP_477152.1 APC-like, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477152.1

Status: REVIEWED

UniProtKB/TrEMBL

P91667, Q9VAS9

Related

FBpp0084720

Conserved Domains (3) summary

cd00020
Location:506 → 624

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam05923
Location:903 → 927

APC_crr; APC cysteine-rich region

sd00043
Location:498 → 539

ARM; armadillo repeat [structural motif]
NM_001316494.1 → NP_001303423.1 APC-like, isoform C [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A126GV30

Conserved Domains (4) summary

cd00020
Location:506 → 624

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam05923
Location:903 → 927

APC_crr; APC cysteine-rich region

pfam16629
Location:648 → 694

Arm_APC_u3; Armadillo-associated region on APC

sd00043
Location:498 → 539

ARM; armadillo repeat [structural motif]
NM_001276117.1 → NP_001263046.1 APC-like, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001263046.1

Status: REVIEWED

UniProtKB/TrEMBL

P91667, Q9VAS9

Conserved Domains (3) summary

cd00020
Location:506 → 624

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam05923
Location:903 → 927

APC_crr; APC cysteine-rich region

sd00043
Location:498 → 539

ARM; armadillo repeat [structural motif]