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    TT12 MATE efflux family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 825072, updated on 18-Sep-2024

    Summary

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    Official Symbol
    TT12
    Official Full Name
    MATE efflux family protein
    Primary source
    TAIR:AT3G59030
    Locus tag
    AT3G59030
    See related
    Araport:AT3G59030
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    A. THALIANA TRANSPARENT TESTA; ATTT12; TRANSPARENT TESTA 12
    Summary
    Encodes a proton antiporter. Involved in the transportation of proanthocyanidin precursors into the vacuole. In vitro transport experiments showed that cyanidin-3-O-glucoside (anthocyanin) was an effective substrate, whereas the proanthocyanidin precursor epicatechin was not transported. However catechin-3-O-glucoside inhibited anthocyanin transport in a dose-dependent manner suggesting that glycosylated epicatechin is the in vivo substrate. Recessive mutation has strong reduction of proanthocyanidin deposition in vacuoles and has reduced dormancy. Expressed in the endothelium of ovules and developing seeds.
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    Genomic context

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    See TT12 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    8
    Sequence:
    Chromosome: 3; NC_003074.8 (21819040..21821440)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene pectin methylesterase 61 Neighboring gene ARM repeat superfamily protein Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene ncRNA Neighboring gene polyamine oxidase 3

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Arabidopsis MATE transporter TT12 acts as a vacuolar flavonoid/H+ -antiporter active in proanthocyanidin-accumulating cells of the seed coat. Marinova K, et al. Plant Cell, 2007 Jun. PMID 17601828, Free PMC Article
    2. Identification and biochemical characterization of mutants in the proanthocyanidin pathway in Arabidopsis. Abrahams S, et al. Plant Physiol, 2002 Oct. PMID 12376625, Free PMC Article
    3. The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium. Debeaujon I, et al. Plant Cell, 2001 Apr. PMID 11283341, Free PMC Article
    4. TRANSPARENT TESTA 13 is a tonoplast P3A -ATPase required for vacuolar deposition of proanthocyanidins in Arabidopsis thaliana seeds. Appelhagen I, et al. Plant J, 2015 Jun. PMID 25891958
    5. Metabolic profiling and cytological analysis of proanthocyanidins in immature seeds of Arabidopsis thaliana flavonoid accumulation mutants. Kitamura S, et al. Plant J, 2010 May 1. PMID 20180920

    See all (9) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that Arabidopsis TT12 functions to transport epicatechin 3'-O-glucoside as a precursor for proanthocyanidin biosynthesis.
      Title: MATE transporters facilitate vacuolar uptake of epicatechin 3'-O-glucoside for proanthocyanidin biosynthesis in Medicago truncatula and Arabidopsis.
    2. TT12 is involved in the vacuolar accumulation of proanthocyanidin precursors in the seed.
      Title: The Arabidopsis MATE transporter TT12 acts as a vacuolar flavonoid/H+ -antiporter active in proanthocyanidin-accumulating cells of the seed coat.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001339948.1
    NM_115765.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables antiporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables polyspecific organic cation:proton antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transmembrane transporter activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables xenobiotic transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in flavonoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in flavonoid transport from endoplasmic reticulum to plant-type vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within maintenance of seed dormancy IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within proanthocyanidin biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in proanthocyanidin biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in plant-type vacuole membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    MATE efflux family protein
    NP_191462.1
    • TRANSPARENT TESTA 12 (TT12); CONTAINS InterPro DOMAIN/s: Multi antimicrobial extrusion protein MatE (InterPro:IPR002528); BEST Arabidopsis thaliana protein match is: MATE efflux family protein (TAIR:AT3G21690.1); Has 8592 Blast hits to 8553 proteins in 1935 species: Archae - 188; Bacteria - 5730; Metazoa - 144; Fungi - 324; Plants - 1347; Viruses - 0; Other Eukaryotes - 859 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      21819040..21821440
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_115765.4 → NP_191462.1  MATE efflux family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_191462.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9LYT3
      UniProtKB/TrEMBL
      A0A178VKA2, A0A5S9XM42
      Conserved Domains (1) summary
      cd13132
      Location:51 → 487
      MATE_eukaryotic; Eukaryotic members of the multidrug and toxic compound extrusion (MATE) family

    2. NM_001339948.1 → NP_001325700.1  MATE efflux family protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1I9LM17
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9LYT3.1

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