Cenpf centromere protein F [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cenpfprovided by MGI
Official Full Name: centromere protein Fprovided by MGI
Primary source: MGI:MGI:1313302
See related: Ensembl:ENSMUSG00000026605 AllianceGenome:MGI:1313302
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CENF; Lek1; PRO1779; mitosin; 6530404A22Rik
Summary: Predicted to enable DNA-binding transcription factor binding activity; dynein complex binding activity; and protein homodimerization activity. Acts upstream of or within regulation of striated muscle tissue development. Located in cytoskeleton and pronucleus. Part of ciliary transition fiber. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and limb. Used to study dilated cardiomyopathy. Human ortholog(s) of this gene implicated in Stromme syndrome. Orthologous to human CENPF (centromere protein F). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in CNS E11.5 (RPKM 9.3), liver E14 (RPKM 4.4) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 H6; 1 95.03 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (189372803..189420302, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (189640606..189688107, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.
Title: CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.
Apoptosis-induced translocation of centromere protein F in its corresponding autoantibody production in hepatocellular carcinoma.
Title: Apoptosis-induced translocation of centromere protein F in its corresponding autoantibody production in hepatocellular carcinoma.
Loss of CENPF leads to developmental failure in mouse embryos.
Title: Loss of CENPF leads to developmental failure in mouse embryos.
Loss of CENP-F Function Induces Kidney Enlargement.
Title: Malformations in the Murine Kidney Caused by Loss of CENP-F Function.
Results found that loss of function mutation in CENPF results in dilated cardiomyopathy with significant disruption in the microtubule network of cardiac myocytes in mouse.
Title: Loss of CENP-F Results in Dilated Cardiomyopathy with Severe Disruption of Cardiac Myocyte Architecture.
Due to the multifunctionality of CENP-F, the cellular phenotypes observed upon its depletion are difficult to interpret and there is a need to genetically separate its different functions by preventing binding to selected partners. Here we engineer a CENP-F point-mutant that is deficient in Miro1/2 binding and thus is unable to localize to mitochondria, but retains other localizations.
Title: Miro-dependent mitochondrial pool of CENP-F and its farnesylated C-terminal domain are dispensable for normal development in mice.
Our data show that mutation of the CENP-F gene leads to an unexpected hyperstabilization of the MT network, with a unique loss of dynamic instability. With disruption of MT dynamics, CENP-F-/- cells exhibit dramatic loss of directionally persistent migration, defects in focal adhesion disassembly and lamellipodial formation/retraction, change in cilia frequency, and loss of regulation of cell shape.
Title: Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities.
FOXM1 and CENPF are master regulators of prostate cancer malignancy, and can serve as drug response markers for antineoplastic drugs efficiency.
Title: Predicting Drug Response in Human Prostate Cancer from Preclinical Analysis of In Vivo Mouse Models.
Loss of CENP-F function in developing myocytes leads to decreased cell division, blunting of trabeculation and an initially smaller, thin-walled heart.
Title: Cardiac-specific deletion of the microtubule-binding protein CENP-F causes dilated cardiomyopathy.
These data demonstrate that CMF1 regulates myocyte differentiation by interaction with Rb family members to induce expression of myogenic regulatory factors.
Title: CMF1-Rb interaction promotes myogenesis in avian skeletal myoblasts.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

Cenpf (e-PCR), detects polymorphism
- UniSTS:527284

AI325968 (e-PCR)
- UniSTS:158906

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables dynein complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables dynein complex binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in metaphase chromosome alignment	IBA Inferred from Biological aspect of Ancestor more info
involved_in metaphase chromosome alignment	ISO Inferred from Sequence Orthology more info
involved_in mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in protein transport	ISO Inferred from Sequence Orthology more info
involved_in regulation of G2/M transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of striated muscle tissue development	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in axoneme	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in chromosome, centromeric region	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome, centromeric region	ISO Inferred from Sequence Orthology more info	PubMed
located_in ciliary basal body	IDA Inferred from Direct Assay more info	PubMed
part_of ciliary transition fiber	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
NOT located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	ISO Inferred from Sequence Orthology more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info	PubMed
part_of outer kinetochore	ISO Inferred from Sequence Orthology more info
located_in pronucleus	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	ISO Inferred from Sequence Orthology more info
is_active_in spindle pole	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: centromere protein F

Names: centromere autoantigen F; leucine, glutamic acid, lysine family 1 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081363.2 → NP_001074832.2 centromere protein F

Status: VALIDATED

Source sequence(s)

AC140679

Consensus CDS

CCDS35820.2

UniProtKB/TrEMBL

E9Q3P4, Q155P7

Related

ENSMUSP00000129738.2, ENSMUST00000171929.8

Conserved Domains (5) summary

COG0419
Location:915 → 1363

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

TIGR02168
Location:290 → 1122

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10473
Location:2313 → 2449

CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1

pfam10481
Location:1 → 300

CENP-F_N; Cenp-F N-terminal domain

pfam10490
Location:2850 → 2896

CENP-F_C_Rb_bdg; Rb-binding domain of kinetochore protein Cenp-F/LEK1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

189372803..189420302 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006497119.4 → XP_006497182.1 centromere protein F isoform X1

UniProtKB/TrEMBL

Q155P7

Conserved Domains (6) summary

COG0419
Location:843 → 1291

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

COG1196
Location:1865 → 2702

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:218 → 1050

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10473
Location:2241 → 2377

CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1

pfam10481
Location:1 → 228

CENP-F_N; Cenp-F N-terminal domain

pfam10490
Location:2778 → 2824

CENP-F_C_Rb_bdg; Rb-binding domain of kinetochore protein Cenp-F/LEK1