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    gcl germ cell-less [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 35864, updated on 9-Dec-2024

    Summary

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    Official Symbol
    gclprovided by FlyBase
    Official Full Name
    germ cell-lessprovided by FlyBase
    Primary source
    FLYBASE:FBgn0005695
    Locus tag
    Dmel_CG8411
    See related
    AllianceGenome:FB:FBgn0005695
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG8411; Dmel\CG8411; Gcl; GCL; gcl-1
    Summary
    Enables ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including negative regulation of torso signaling pathway; negative regulation of transcription by RNA polymerase III; and pole cell fate determination. Located in cell cortex and nuclear periphery. Part of Cul3-RING ubiquitin ligase complex and nuclear pore. Is expressed in several structures, including embryonic dorsal epidermis; embryonic/larval midgut primordium; ganglia; germline cell; and gut section. Orthologous to human GMCL1 (germ cell-less 1, spermatogenesis associated) and GMCL2 (germ cell-less 2, spermatogenesis associated). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See gcl in Genome Data Viewer
    Location:
    44E3-44E3; 2-59 cM
    Exon count:
    5
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (8724258..8728999)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (4611763..4616504)

    Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene Peptidoglycan recognition protein SC2 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene ncRNA Neighboring gene uncharacterized protein Neighboring gene stambha A Neighboring gene uncharacterized protein Neighboring gene bloated

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A spindle-independent cleavage pathway controls germ cell formation in Drosophila. Cinalli RM, et al. Nat Cell Biol, 2013 Jul. PMID 23728423, Free PMC Article
    2. No extension of lifespan by ablation of germ line in Drosophila. Barnes AI, et al. Proc Biol Sci, 2006 Apr 22. PMID 16627279, Free PMC Article
    3. Germ Cell Segregation from the Drosophila Soma Is Controlled by an Inhibitory Threshold Set by the Arf-GEF Steppke. Lee DM, et al. Genetics, 2015 Jul. PMID 25971667, Free PMC Article
    4. Germ Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation. Lerit DA, et al. Cell Rep, 2017 Jan 24. PMID 28122234, Free PMC Article
    5. Distinct cis-acting elements mediate targeting and clustering of Drosophila polar granule mRNAs. Eagle WVI, et al. Development, 2018 Nov 22. PMID 30333216, Free PMC Article

    See all (131) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Antagonism between germ cell-less and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.
      Title: Antagonism between germ cell-less and Torso receptor regulates transcriptional quiescence underlying germline/soma distinction.
    2. CRL3(GCL) promotes primordial germ cell fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate.
      Title: GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.
    3. Repression by Bruno during oogenesis is required to restrict Gcl expression in the early embryo.
      Title: Bruno negatively regulates germ cell-less expression in a BRE-independent manner.
    4. Germ cell-less (GCL), a germ plasm component necessary for the proper formation of "pole cells," the germ cell precursors in Drosophila, is required for the establishment of this transcriptional quiescence
      Title: germ cell-less acts to repress transcription during the establishment of the Drosophila germ cell lineage.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables ubiquitin-like ligase-substrate adaptor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in germ cell development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in germ cell development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated transcription TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of torso signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of transcription by RNA polymerase III IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pole cell fate determination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in pole cell fate determination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in pole cell formation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein polyubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Cul3-RING ubiquitin ligase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cell cortex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear periphery IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of nuclear pore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    germ cell-less
    Names
    CG8411-PA
    CG8411-PB
    gcl-PA
    gcl-PB
    germ cell less
    germ-cell-less

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033778.4 Reference assembly

      Range
      8724258..8728999
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001299281.1 → NP_001286210.1  germ cell-less, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286210.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4LEY8
      Conserved Domains (2) summary
      cd18305
      Location:51 → 165
      BTB_POZ_GCL; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Drosophila melanogaster protein germ cell-less (GCL) and similar proteins
      cd18495
      Location:166 → 245
      BACK_GCL; BACK (BTB and C-terminal Kelch) domain found in Drosophila melanogaster protein germ cell-less (GCL) and similar proteins

    2. NM_165622.2 → NP_724708.1  germ cell-less, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_724708.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q01820, Q9V4X5
      Related
      FBpp0087789
      Conserved Domains (2) summary
      smart00225
      Location:67 → 166
      BTB; Broad-Complex, Tramtrack and Bric a brac
      pfam00651
      Location:56 → 163
      BTB; BTB/POZ domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A0A0B4LEY8 A0A0B4LEY8
    Q01820.1 Q01820

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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