KAK HECT ubiquitin protein ligase family protein KAK [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: KAK
Official Full Name: HECT ubiquitin protein ligase family protein KAK
Primary source: TAIR:AT4G38600
Locus tag: AT4G38600
See related: Araport:AT4G38600
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AT4G38610; F20M13.160; F20M13_160; KAKTUS; UBIQUITIN-PROTEIN LIGASE 3; UBIQUITIN_PROTEIN LIGASE; UPL3
Summary: encodes a member of HECT ubiquitin protein ligase family that is involved in trichome cell morphogenesis. Mutants in this gene exhibit supernumerary trichome branches and increased DNA content.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See KAK in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 18

Sequence:: Chromosome: 4; NC_003075.7 (18040623..18049700, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HECT-type ubiquitin ligase KAKTUS mediates the proteasome-dependent degradation of cyclin-dependent kinase inhibitor KRP2 during trichome morphogenesis in Arabidopsis.
Title: HECT-type ubiquitin ligase KAKTUS mediates the proteasome-dependent degradation of cyclin-dependent kinase inhibitor KRP2 during trichome morphogenesis in Arabidopsis.
a recessive mutation in the KAKTUS (KAK) gene that suppressed dwarfism and the collapsed xylem character, the cause of decreased hydraulic conductivity in the esk1-5 mutant.
Title: Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis.
Mutation in the gl3 locus negatively affects UPL3 expression, whereas overexpression of GL3 moderately upregulates it, suggesting the presence of a regulatory loop involving GL3 and UPL3.
Title: Ubiquitin protein ligase 3 mediates the proteasomal degradation of GLABROUS 3 and ENHANCER OF GLABROUS 3, regulators of trichome development and flavonoid biosynthesis in Arabidopsis.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

NM_120021.2

Supported by ESTs:

NM_179236.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA endoreduplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within trichome branching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info
located_in plasma membrane	HDA more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: HECT ubiquitin protein ligase family protein KAK

NP_195572.2

KAKTUS (KAK); FUNCTIONS IN: ubiquitin-protein ligase activity; INVOLVED IN: trichome branching, DNA endoreduplication; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Armadillo-like helical (InterPro:IPR011989), Armadillo (InterPro:IPR000225), Armadillo-type fold (InterPro:IPR016024), HECT (InterPro:IPR000569); BEST Arabidopsis thaliana protein match is: ubiquitin-protein ligase 4 (TAIR:AT5G02880.1); Has 6273 Blast hits to 5534 proteins in 324 species: Archae - 2; Bacteria - 240; Metazoa - 3391; Fungi - 909; Plants - 600; Viruses - 3; Other Eukaryotes - 1128 (source: NCBI BLink).

NP_849567.2

KAKTUS (KAK); FUNCTIONS IN: ubiquitin-protein ligase activity; INVOLVED IN: trichome branching, DNA endoreduplication; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Armadillo-like helical (InterPro:IPR011989), Armadillo (InterPro:IPR000225), Armadillo-type fold (InterPro:IPR016024), HECT (InterPro:IPR000569); BEST Arabidopsis thaliana protein match is: ubiquitin-protein ligase 4 (TAIR:AT5G02880.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

18040623..18049700 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001342508.1 → NP_001329354.1 HECT ubiquitin protein ligase family protein KAK [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WN20, Q6WWW4, Q9SZN9, Q9SZP0

UniProtKB/TrEMBL

A0A7G2F717
NM_179236.3 → NP_849567.2 HECT ubiquitin protein ligase family protein KAK [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_849567.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WN20, Q6WWW4, Q9SZN9, Q9SZP0

UniProtKB/TrEMBL

A0A7G2F717

Conserved Domains (7) summary

smart00119
Location:1494 → 1883

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

COG5064
Location:211 → 611

SRP1; Karyopherin (importin) alpha [Intracellular trafficking and secretion]

cd00020
Location:239 → 348

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

cd00078
Location:1471 → 1883

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam13513
Location:336 → 388

HEAT_EZ; HEAT-like repeat

sd00044
Location:361 → 387

HEAT; HEAT repeat [structural motif]

sd00043
Location:273 → 310

ARM; armadillo repeat [structural motif]
NM_120021.2 → NP_195572.2 HECT ubiquitin protein ligase family protein KAK [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_195572.2

Status: REVIEWED

UniProtKB/TrEMBL

A0A7G2F717, B3H700

Conserved Domains (7) summary

smart00119
Location:1403 → 1789

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

COG5064
Location:138 → 538

SRP1; Karyopherin (importin) alpha [Intracellular trafficking and secretion]

cd00020
Location:166 → 275

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

cd00078
Location:1380 → 1789

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam13513
Location:263 → 315

HEAT_EZ; HEAT-like repeat

sd00044
Location:288 → 314

HEAT; HEAT repeat [structural motif]

sd00043
Location:200 → 237

ARM; armadillo repeat [structural motif]