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    HOT5 GroES-like zinc-binding dehydrogenase family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 834417, updated on 18-Sep-2024

    Summary

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    Official Symbol
    HOT5
    Official Full Name
    GroES-like zinc-binding dehydrogenase family protein
    Primary source
    TAIR:AT5G43940
    Locus tag
    AT5G43940
    See related
    Araport:AT5G43940
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ADH2; ALCOHOL DEHYDROGENASE 2; ATGSNOR1; FALDH; GLUTATHIONE-DEPENDENT FORMALDEHYDE DEHYDROGENASE; GSNOR; MRH10.4; MRH10_4; PAR2; PARAQUAT RESISTANT 2; S-NITROSOGLUTATHIONE REDUCTASE; sensitive to hot temperatures 5
    Summary
    Encodes a glutathione-dependent formaldehyde dehydrogenase (also known as class III type alcohol dehydrogenase) reduces S-nitrosoglutathione (GSNO), the condensation product of glutathione and NO, that is a naturally occurring NO reservoir and also a reactive nitrogen intermediate. Gene expression is reduced by wounding and induced by salicylic acid. Is required for the acclimation of plants to high temperature and for fertility.
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    Genomic context

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    See HOT5 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    9
    Sequence:
    Chromosome: 5; NC_003076.8 (17684156..17686534)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene flavonol synthase 6 Neighboring gene vacuolar protein sorting-associated protein (DUF946) Neighboring gene Nuclear transport factor 2 (NTF2) family protein with RNA binding (RRM-RBD-RNP motifs) domain-containing protein Neighboring gene translocase of outer membrane 22-V

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. AtGSNOR1 function is required for multiple developmental programs in Arabidopsis. Kwon E, et al. Planta, 2012 Sep. PMID 22767201
    2. Arabidopsis formaldehyde dehydrogenase. Molecular properties of plant class III alcohol dehydrogenase provide further insights into the origins, structure and function of plant class p and liver class I alcohol dehydrogenases. Martínez MC, et al. Eur J Biochem, 1996 Nov 1. PMID 8944774
    3. Cloning of the Arabidopsis and rice formaldehyde dehydrogenase genes: implications for the origin of plant ADH enzymes. Dolferus R, et al. Genetics, 1997 Jul. PMID 9215914, Free PMC Article
    4. S-nitrosoglutathione reductase affords protection against pathogens in Arabidopsis, both locally and systemically. Rustérucci C, et al. Plant Physiol, 2007 Mar. PMID 17277089, Free PMC Article
    5. Modulation of nitrosative stress by S-nitrosoglutathione reductase is critical for thermotolerance and plant growth in Arabidopsis. Lee U, et al. Plant Cell, 2008 Mar. PMID 18326829, Free PMC Article

    See all (22) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. ADH2/GSNOR1 is a key player in limiting genotoxic damage mediated by formaldehyde and UV-B in Arabidopsis.
      Title: ADH2/GSNOR1 is a key player in limiting genotoxic damage mediated by formaldehyde and UV-B in Arabidopsis.
    2. Induction of S-nitrosoglutathione reductase protects root growth from ammonium toxicity by regulating potassium homeostasis in Arabidopsis and rice.
      Title: Induction of S-nitrosoglutathione reductase protects root growth from ammonium toxicity by regulating potassium homeostasis in Arabidopsis and rice.
    3. Glutathione-dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H2 O2.
      Title: Glutathione-dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H(2) O(2).
    4. GSNOR provides plant tolerance to iron toxicity via preventing iron-dependent nitrosative and oxidative cytotoxicity.
      Title: GSNOR provides plant tolerance to iron toxicity via preventing iron-dependent nitrosative and oxidative cytotoxicity.
    5. discovery reveals a unique mechanism by which S-nitrosylation mediates selective autophagy of GSNOR1, thereby establishing a molecular link between NO signaling and autophagy.
      Title: S-Nitrosylation Targets GSNO Reductase for Selective Autophagy during Hypoxia Responses in Plants.
    6. polar auxin transport was compromised in gsnor1-3, which was correlated with universally reduced levels of PIN or GFP-PIN proteins in the roots of the mutant.
      Title: Loss of GSNOR1 Function Leads to Compromised Auxin Signaling and Polar Auxin Transport.
    7. Collectively, our findings imply that AtGSNOR1 controls multiple genetic networks integral to plant growth and development.
      Title: AtGSNOR1 function is required for multiple developmental programs in Arabidopsis.
    8. Data indicate that after avirulent infection, 38 protein spots in WT plants, but only 18 protein spots in atgsnor plants showed altered abundance.
      Title: Proteomic analysis of defense response of wildtype Arabidopsis thaliana and plants with impaired NO- homeostasis.
    9. Taken together, these results suggest that GSNOR1/HOT5/PAR2 plays an important role in regulating cell death in plant cells through modulating intracellular NO level.
      Title: The Arabidopsis PARAQUAT RESISTANT2 gene encodes an S-nitrosoglutathione reductase that is a key regulator of cell death.
    10. S-nitrosoglutathione reductase is an important and widely utilized component of pathogen resistance protein signaling networks conserved in animals and plants.
      Title: S-nitrosoglutathione reductase affords protection against pathogens in Arabidopsis, both locally and systemically.
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    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_123761.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables S-nitrosoglutathione reductase (NADH) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within formaldehyde metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within formaldehyde metabolic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within heat acclimation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within programmed cell death IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within seed development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm ISM
    Inferred from Sequence Model
    more info
    		  
    located_in cytosol HDA PubMed 
    located_in peroxisome HDA PubMed 
    located_in plastid HDA PubMed 

    General protein information

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    Preferred Names
    GroES-like zinc-binding dehydrogenase family protein
    NP_001190468.1
    • sensitive to hot temperatures 5 (HOT5); CONTAINS InterPro DOMAIN/s: GroES-like (InterPro:IPR011032), Alcohol dehydrogenase, zinc-containing, conserved site (InterPro:IPR002328), Alcohol dehydrogenase GroES-like (InterPro:IPR013154), Alcohol dehydrogenase, C-terminal (InterPro:IPR013149), Alcohol dehydrogenase class III/S-(hydroxymethyl)glutathione dehydrogenase (InterPro:IPR014183), Alcohol dehydrogenase superfamily, zinc-containing (InterPro:IPR002085); BEST Arabidopsis thaliana protein match is: alcohol dehydrogenase 1 (TAIR:AT1G77120.1).
    NP_199207.1
    • sensitive to hot temperatures 5 (HOT5); FUNCTIONS IN: S-(hydroxymethyl)glutathione dehydrogenase activity, S-nitrosoglutathione reductase activity; INVOLVED IN: in 6 processes; LOCATED IN: peroxisome; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: GroES-like (InterPro:IPR011032), Alcohol dehydrogenase GroES-like (InterPro:IPR013154), Alcohol dehydrogenase, zinc-containing, conserved site (InterPro:IPR002328), Alcohol dehydrogenase, C-terminal (InterPro:IPR013149), Alcohol dehydrogenase class III/S-(hydroxymethyl)glutathione dehydrogenase (InterPro:IPR014183), Alcohol dehydrogenase superfamily, zinc-containing (InterPro:IPR002085); BEST Arabidopsis thaliana protein match is: alcohol dehydrogenase 1 (TAIR:AT1G77120.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      17684156..17686534
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001203539.1 → NP_001190468.1  GroES-like zinc-binding dehydrogenase family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001190468.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      F4K7D6
      Conserved Domains (2) summary
      TIGR02818
      Location:24 → 387
      adh_III_F_hyde; S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase
      cd08300
      Location:8 → 388
      alcohol_DH_class_III; class III alcohol dehydrogenases

    2. NM_123761.4 → NP_199207.1  GroES-like zinc-binding dehydrogenase family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_199207.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q0WWE1, Q43384, Q96533, Q9FND2
      UniProtKB/TrEMBL
      A0A178URC5, A0A5S9YD31
      Conserved Domains (1) summary
      cd08300
      Location:8 → 376
      alcohol_DH_class_III; class III alcohol dehydrogenases
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q96533.2

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