HEMA1 Glutamyl-tRNA reductase family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: HEMA1
Official Full Name: Glutamyl-tRNA reductase family protein
Primary source: TAIR:AT1G58290
Locus tag: AT1G58290
See related: Araport:AT1G58290
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: Arabidopsis thaliana hemA 1; AtHEMA1; F19C14.9; F19C14_9
Summary: Encodes a protein with glutamyl-tRNA reductase (GluTR) activity, catalyzing the NADPH-dependent reduction of Glu-tRNA(Glu) to glutamate 1-semialdehyde (GSA) with the release of free tRNA(Glu). It is involved in the early steps of chlorophyll biosynthesis.
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Genomic context

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See HEMA1 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 3

Sequence:: Chromosome: 1; NC_003070.9 (21623700..21626402, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

binding of heme to the GluTR-binding protein (GBP) inhibits interaction of GBP with the N-terminal regulatory domain of GluTR1, thus making it accessible to the Clp protease.[GluTR1]
Title: The GluTR-binding protein is the heme-binding factor for feedback control of glutamyl-tRNA reductase.
HemA1 and pgr7 genes from the higher plant Arabidopsis thaliana into recombinant Escherichia coli to overproduce extracellular 5-aminolevulinic acid via the C5 pathway.
Title: Production of 5-aminolevulinic acid from glutamate by overexpressing HemA1 and pgr7 from Arabidopsis thaliana in Escherichia coli.
the proteolytic activity of Clp protease counteracts GBP binding to assure the appropriate content of GluTR and the adequate amino levulinic acid synthesis for chlorophyll and heme in higher plants.
Title: Posttranslational Control of ALA Synthesis Includes GluTR Degradation by Clp Protease and Stabilization by GluTR-Binding Protein.
a ternary complex composed of the C-terminal tetratricopepetide-repeat domain of FLU, GBP, and GluTR, is reported
Title: The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein.
characterize the FLU-GluTR interaction by solving the crystal structures of the uncomplexed TPR domain of FLU (FLU(TPR)) at 1.45-A resolution and the complex of the dimeric domain of GluTR bound to FLU(TPR) at 2.4-A resolution
Title: The Non-canonical Tetratricopeptide Repeat (TPR) Domain of Fluorescent (FLU) Mediates Complex Formation with Glutamyl-tRNA Reductase.
A gene encoding aminolevulinate synthase (ALA-S) in yeast (Saccharomyces cerevisiae YHem1) was introduced into the genome of tobacco (Nicoliana tabacum) under the control of Arabidopsis thaliana HemA1 gene promoter (AtHemA1 P).
Title: Expression of yeast Hem1 controlled by Arabidopsis HemA1 promoter enhances leaf photosynthesis in transgenic tobacco.
HEMA1 overexpression in etiolated and dark-grown Arabidopsis and tobacco seedlings leads to additional accumulation of protochlorophyllide.
Title: Overexpression of HEMA1 encoding glutamyl-tRNA reductase.
Analysis of HEMA1 expression in gun1 seedlings under non-photobleaching conditions indicates that GUN1 is an important suppressor of HEMA1 expression in the dark and under saturating white light
Title: The nuclear genes Lhcb and HEMA1 are differentially sensitive to plastid signals and suggest distinct roles for the GUN1 and GUN5 plastid-signalling pathways during de-etiolation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_104609.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables glutamyl-tRNA reductase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables glutamyl-tRNA reductase activity	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chlorophyll biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chlorophyll biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heme biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within porphyrin-containing compound biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in protoporphyrinogen IX biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to light stimulus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast	TAS Traceable Author Statement more info	PubMed
part_of protein-containing complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: Glutamyl-tRNA reductase family protein

NP_176125.1

HEMA1; FUNCTIONS IN: glutamyl-tRNA reductase activity; INVOLVED IN: chlorophyll biosynthetic process, heme biosynthetic process, response to light stimulus, porphyrin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase (InterPro:IPR006151), Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, conserved site (InterPro:IPR018214), Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, C-terminal (InterPro:IPR015896), NAD(P)-binding domain (InterPro:IPR016040), Tetrapyrrole biosynthesis, glutamyl-tRNA reductase (InterPro:IPR000343), Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, N-terminal (InterPro:IPR015895); BEST Arabidopsis thaliana protein match is: Glutamyl-tRNA reductase family protein (TAIR:AT1G09940.1); Has 5070 Blast hits to 5064 proteins in 1817 species: Archae - 226; Bacteria - 3676; Metazoa - 1; Fungi - 0; Plants - 227; Viruses - 0; Other Eukaryotes - 940 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

21623700..21626402 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_104609.4 → NP_176125.1 Glutamyl-tRNA reductase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_176125.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P42804, Q0WWL6, Q9LQB9

UniProtKB/TrEMBL

A0A5S9WQI4, A0A654EJU2

Conserved Domains (1) summary

PLN00203
Location:1 → 530

PLN00203; glutamyl-tRNA reductase