NLA SPX (SYG1/Pho81/XPR1) domain-containing protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: NLA
Official Full Name: SPX (SYG1/Pho81/XPR1) domain-containing protein
Primary source: TAIR:AT1G02860
Locus tag: AT1G02860
See related: Araport:AT1G02860
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: BAH1; BENZOIC ACID HYPERSENSITIVE 1; F22D16.14; F22D16_14; nitrogen limitation adaptation
Summary: Encodes a ubiquitin E3 ligase with RING and SPX domains that is involved in mediating immune responses and mediates degradation of PHT1s at plasma membranes. Targeted by MIR827. Ubiquitinates PHT1;3, PHT1;2, PHT1;1/AtPT1 and PHT1;4/AtPT2.
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Genomic context

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See NLA in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 6

Sequence:: Chromosome: 1; NC_003070.9 (635144..637262)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inositol polyphosphates-regulated polyubiquitination of PHR1 by NLA E3 ligase during phosphate starvation response in Arabidopsis.
Title: Inositol polyphosphates-regulated polyubiquitination of PHR1 by NLA E3 ligase during phosphate starvation response in Arabidopsis.
regulates ORE1 homeostasis during senescence induced by nitrogen deficiency
Title: Arabidopsis NITROGEN LIMITATION ADAPTATION regulates ORE1 homeostasis during senescence induced by nitrogen deficiency.
BAH1, an E3 ligase from plant that has a similar zinc finger domain to DnaJ, can perform block the effect of DnaK on sigma(32) in Escherichia coli.
Title: BAH1 an E3 Ligase from Arabidopsis thaliana Stabilizes Heat Shock Factor σ(32) of Escherichia coli by Interacting with DnaK/DnaJ Chaperone Team.
NLA interacts with NRT1.7 in the plasma membrane.
Title: Nitrogen Limitation Adaptation (NLA) is involved in source-to-sink remobilization of nitrate by mediating the degradation of NRT1.7 in Arabidopsis.
NLA is an E3 ligase that specifically requires UBC24 for polyubiquitination in Arabidopsis thaliana.
Title: NITROGEN LIMITATION ADAPTATION recruits PHOSPHATE2 to target the phosphate transporter PT2 for degradation during the regulation of Arabidopsis phosphate homeostasis.
NLA is important for regulating the abundance of PHT1 proteins to maintain Pi homeostasis
Title: Nitrogen limitation adaptation, a target of microRNA827, mediates degradation of plasma membrane-localized phosphate transporters to maintain phosphate homeostasis in Arabidopsis.
NLA and miR827 have pivotal roles in regulating Pi homeostasis in plants in a nitrate-dependent fashion
Title: Genetic regulation by NLA and microRNA827 for maintaining nitrate-dependent phosphate homeostasis in arabidopsis.
BAH1/NLA plays a crucial role in the ubiquitination-mediated regulation of immune response, and regulates the accumulation of salicylic acid.
Title: BAH1/NLA, a RING-type ubiquitin E3 ligase, regulates the accumulation of salicylic acid and immune responses to Pseudomonas syringae DC3000.
controls Arabidopsis adaptability to nitrogen limitation by channelling the phenylpropanoid metabolic flux to the induced anthocyanin synthesis
Title: Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene.
Nitrogen limitation triggers extensive transcriptome response in Arabidopsis plants and is regulated by NLA. [NLA]
Title: Genome-wide analysis of Arabidopsis responsive transcriptome to nitrogen limitation and its regulation by the ubiquitin ligase gene NLA.

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General gene information

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Homology

Orthologs from OrthoDB

NM_001123746.1

Supported by ESTs:

NM_001331361.1

Supported by ESTs:

NM_100167.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin-protein transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin-protein transferase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within plant-type hypersensitive response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of salicylic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to benzoic acid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to benzoic acid	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to nitrate	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to salicylic acid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within salicylic acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within systemic acquired resistance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in mitochondrion	ISM Inferred from Sequence Model more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: SPX (SYG1/Pho81/XPR1) domain-containing protein

NP_001117218.1

nitrogen limitation adaptation (NLA); CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type, conserved site (InterPro:IPR017907), Zinc finger, RING-type (InterPro:IPR001841), SPX, N-terminal (InterPro:IPR004331); BEST Arabidopsis thaliana protein match is: SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING finger) protein-related (TAIR:AT2G38920.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_563667.1

nitrogen limitation adaptation (NLA); CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type, conserved site (InterPro:IPR017907), Zinc finger, RING-type (InterPro:IPR001841), SPX, N-terminal (InterPro:IPR004331); BEST Arabidopsis thaliana protein match is: SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING finger) protein-related (TAIR:AT2G38920.1); Has 2026 Blast hits to 2013 proteins in 210 species: Archae - 0; Bacteria - 0; Metazoa - 1472; Fungi - 138; Plants - 261; Viruses - 0; Other Eukaryotes - 155 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

635144..637262

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_100167.3 → NP_563667.1 SPX (SYG1/Pho81/XPR1) domain-containing protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_563667.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B3H5D0, Q8L8T3, Q9SRX9

UniProtKB/TrEMBL

A0A178WJE5, A0A5S9SBB1

Conserved Domains (2) summary

COG5574
Location:228 → 297

PEX10; RING-finger-containing E3 ubiquitin ligase [Posttranslational modification, protein turnover, chaperones]

cd14482
Location:2 → 150

SPX_BAH1-like; SPX domain of the E3 ubiquitin-protein ligase BAH1/NLA and similar proteins
NM_001123746.1 → NP_001117218.1 SPX (SYG1/Pho81/XPR1) domain-containing protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001117218.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9SRX9

Conserved Domains (2) summary

cd00162
Location:230 → 282

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cd14482
Location:2 → 150

SPX_BAH1-like; SPX domain of the E3 ubiquitin-protein ligase BAH1/NLA and similar proteins
NM_001331361.1 → NP_001321448.1 SPX (SYG1/Pho81/XPR1) domain-containing protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AR01