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relE mRNA interferase toxin RelE [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947549, updated on 3-Dec-2024

Summary

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Official Symbol
relE
Official Full Name
mRNA interferase toxin RelE
Primary source
ECOCYC:EG11131
Locus tag
b1563
See related
ASAP:ABE-0005221
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1557
Summary
Overproduction of RelE has been shown to increase persistence. [More information is available at EcoGene: EG11131]. RelE is the toxin of the RelE-RelB toxin-antitoxin system . [More information is available at EcoCyc: EG11131].
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Genomic context

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Sequence:
NC_000913.3 (1645346..1645633, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein Rem Neighboring gene toxic protein HokD Neighboring gene antitoxin/DNA-binding transcriptional repressor RelB Neighboring gene protein YdfV

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Transition State Charge Stabilization and Acid-Base Catalysis of mRNA Cleavage by the Endoribonuclease RelE. Dunican BF, et al. Biochemistry, 2015 Dec 1. PMID 26535789, Free PMC Article
  2. The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome. Andreev D, et al. RNA, 2008 Feb. PMID 18083838, Free PMC Article
  3. Delayed-relaxed response explained by hyperactivation of RelE. Christensen SK, et al. Mol Microbiol, 2004 Jul. PMID 15228536
  4. Toxin-antitoxin pairs in bacteria: killers or stress regulators? Hayes CS, et al. Cell, 2003 Jan 10. PMID 12526786
  5. Bacterial toxin RelE induces apoptosis in human cells. Yamamoto TA, et al. FEBS Lett, 2002 May 22. PMID 12023043

See all (57) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A ribosome profiling study of mRNA cleavage by the endonuclease RelE has been reported.
    Title: A ribosome profiling study of mRNA cleavage by the endonuclease RelE.
  2. The study uses a single-turnover kinetic analysis to measure the effect of pH and phosphorothioate substitution on the rate constant for cleavage of mRNA by wild-type RelE and seven active-site mutants.
    Title: Transition State Charge Stabilization and Acid-Base Catalysis of mRNA Cleavage by the Endoribonuclease RelE.
  3. RelE may principally promote catalysis via transition-state charge stabilization and leaving-group protonation, in addition to achieving in-line substrate positioning in cooperation with the ribosome.
    Title: Bacterial toxin RelE: a highly efficient ribonuclease with exquisite substrate specificity using atypical catalytic residues.
  4. RelE-like toxins have evolved to rapidly and efficiently shut down translation in a large spectrum of bacterial species, which correlates with the observation that toxin-antitoxin systems are spreading by horizontal gene transfer.
    Title: Relaxed cleavage specificity within the RelE toxin family.
  5. Here, the authors report that the transition to dormant states is mediated by one of the major toxin-antitoxin systems, RelEB, in a cell density-dependent manner in Escherichia coli K-12 MG1655.
    Title: RelE-mediated dormancy is enhanced at high cell density in Escherichia coli.
  6. RelE occupies the A site and causes cleavage of mRNA after the second nucleotide of the codon by reorienting and activating the mRNA for 2'-OH-induced hydrolysis.
    Title: The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE.
  7. molecular interactions important for transcriptional control of the relBE model operon were characterized.
    Title: RelB and RelE of Escherichia coli form a tight complex that represses transcription via the ribbon-helix-helix motif in RelB.
  8. This study analyses mechanistic aspects of how RelE of Escherichia coli can function both as a co-repressor and as a derepressor of relBE transcription
    Title: Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity.
  9. RelE specifically cleaves mRNA in the A site of the eukaryote ribosome.
    Title: The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome.
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General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA-binding transcription repressor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables DNA-binding transcription repressor activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables RNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables ribosome binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables toxin sequestering activity EXP
Inferred from Experiment
more info
 PubMed 
enables transcription cis-regulatory region binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables transcription cis-regulatory region binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within cellular response to amino acid starvation IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in mRNA catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within mRNA catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within negative regulation of translation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in regulation of growth NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to antibiotic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in single-species biofilm formation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of protein-DNA complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of protein-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of toxin-antitoxin complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
mRNA interferase toxin RelE

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1645346..1645633 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416081.1 mRNA interferase toxin RelE [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416081.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AAI9H3H8
    Conserved Domains (1) summary
    COG2026
    Location:1 → 87
    RelE; mRNA-degrading endonuclease RelE, toxin component of the RelBE toxin-antitoxin system [Defense mechanisms]
Nucleotide Protein
Heading Accession and Version

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