TPL Transducin family protein / WD-40 repeat family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: TPL
Official Full Name: Transducin family protein / WD-40 repeat family protein
Primary source: TAIR:AT1G15750
Locus tag: AT1G15750
See related: Araport:AT1G15750
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: F7H2.9; F7H2_9; TOPLESS; WSIP1; WUS-INTERACTING PROTEIN 1
Summary: Encodes a protein with several WD40 repeats at the C-terminus and predicted protein-protein interaction domains at the N-terminus. Together with the TOPLESS-RELATED PROTEINS (TPRs), it is thought to be involved in transcriptional repression of root-promoting genes in the top half of the embryo during the transition stage of embryogenesis. It can also interact with IAA12 through the EAR domain of IAA12 and the CTLH domain of TPL. The ability of IAA12 to repress transcription is diminished in a tpl-1 mutant background.
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Genomic context

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See TPL in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 24

Sequence:: Chromosome: 1; NC_003070.9 (5414817..5421033, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex.
Title: Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex.
These results suggested that integrating activation of Brassinosteroid (BR)-signaling pathways with the formation of the protein complex containing BES1/BZR1 and TPL-HDA19 via the EAR motif was important in fine-tuning BR-related gene networks in plants.
Title: Transcriptional network regulation of the brassinosteroid signaling pathway by the BES1-TPL-HDA19 co-repressor complex.
the 1st WD40 propeller of TPL can form a complex with RAV1 both in yeast and in Arabidopsis protoplasts.
Title: The C-terminal WD40 repeats on the TOPLESS co-repressor function as a protein-protein interaction surface.
relative to mammalian TBL1, evolved a new tetramerization interface and unique and highly conserved surface for interaction with repressors
Title: Structure of the Arabidopsis TOPLESS corepressor provides insight into the evolution of transcriptional repression.
The GIR1 and GIR2 might may link TPL to and participate in epigenetic regulation of root hair development.
Title: Adaptor proteins GIR1 and GIR2. II. Interaction with the co-repressor TOPLESS and promotion of histone deacetylation of target chromatin.
These data unveil a pivotal role of the co-repressor TPL in the shoot and root meristems.
Title: TOPLESS mediates brassinosteroid control of shoot boundaries and root meristem development in Arabidopsis thaliana.
Interaction of CO with miP1a/b/TPL causes late flowering due to a failure in the induction of FLOWERING LOCUS T (FT) expression under inductive long day conditions.
Title: MicroProtein-Mediated Recruitment of CONSTANS into a TOPLESS Trimeric Complex Represses Flowering in Arabidopsis.
Data showed that SPOROCYTELESS (SPL) most likely functions as a transcription repressor by interacting with TCP family transcription factors and the TOPLESS (TPL) co-repressors.
Title: SPOROCYTELESS is a novel embryophyte-specific transcription repressor that interacts with TPL and TCP proteins in Arabidopsis.
TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1.
Title: TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1.
Topless and Topless-related proteins form a tertiary complex with TIE1 protein at early stages of leaf development.
Title: The TIE1 transcriptional repressor links TCP transcription factors with TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001035973.3

Supported by ESTs:

NM_001035974.4

Supported by ESTs:

NM_101443.4

Supported by ESTs:

NM_179341.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within jasmonic acid mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within primary shoot apical meristem specification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within primary shoot apical meristem specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to auxin	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within xylem and phloem pattern formation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Transducin family protein / WD-40 repeat family protein

NP_001031050.1

TOPLESS (TPL); FUNCTIONS IN: protein binding, transcription repressor activity, protein homodimerization activity; INVOLVED IN: xylem and phloem pattern formation, response to auxin stimulus, primary shoot apical meristem specification, jasmonic acid mediated signaling pathway; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), CTLH, C-terminal LisH motif (InterPro:IPR006595), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), LisH dimerisation motif (InterPro:IPR006594), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: TOPLESS-related 1 (TAIR:AT1G80490.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_001031051.1

TOPLESS (TPL); FUNCTIONS IN: protein binding, transcription repressor activity, protein homodimerization activity; INVOLVED IN: xylem and phloem pattern formation, response to auxin stimulus, primary shoot apical meristem specification, jasmonic acid mediated signaling pathway; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), CTLH, C-terminal LisH motif (InterPro:IPR006595), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), LisH dimerisation motif (InterPro:IPR006594), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: TOPLESS-related 1 (TAIR:AT1G80490.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_563981.1

TOPLESS (TPL); FUNCTIONS IN: protein binding, transcription repressor activity, protein homodimerization activity; INVOLVED IN: xylem and phloem pattern formation, response to auxin stimulus, primary shoot apical meristem specification, jasmonic acid mediated signaling pathway; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), CTLH, C-terminal LisH motif (InterPro:IPR006595), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), LisH dimerisation motif (InterPro:IPR006594), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: TOPLESS-related 1 (TAIR:AT1G80490.2); Has 22110 Blast hits to 13392 proteins in 605 species: Archae - 36; Bacteria - 4397; Metazoa - 7838; Fungi - 4804; Plants - 2241; Viruses - 6; Other Eukaryotes - 2788 (source: NCBI BLink).

NP_849672.1

TOPLESS (TPL); FUNCTIONS IN: protein binding, transcription repressor activity, protein homodimerization activity; INVOLVED IN: xylem and phloem pattern formation, response to auxin stimulus, primary shoot apical meristem specification, jasmonic acid mediated signaling pathway; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), CTLH, C-terminal LisH motif (InterPro:IPR006595), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), LisH dimerisation motif (InterPro:IPR006594), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: TOPLESS-related 1 (TAIR:AT1G80490.2); Has 22110 Blast hits to 13392 proteins in 605 species: Archae - 36; Bacteria - 4397; Metazoa - 7838; Fungi - 4804; Plants - 2241; Viruses - 6; Other Eukaryotes - 2788 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

5414817..5421033 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_179341.3 → NP_849672.1 Transducin family protein / WD-40 repeat family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_849672.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94AI7, Q9LMQ9

UniProtKB/TrEMBL

A0A178WLB2

Conserved Domains (5) summary

smart00667
Location:4 → 34

LisH; Lissencephaly type-1-like homology motif

smart00668
Location:34 → 92

CTLH; C-terminal to LisH motif

COG2319
Location:360 → 748

WD40; WD40 repeat [General function prediction only]

sd00039
Location:423 → 460

7WD40; WD40 repeat [structural motif]

cl02567
Location:359 → 667

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001035974.4 → NP_001031051.1 Transducin family protein / WD-40 repeat family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031051.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94AI7, Q9LMQ9

UniProtKB/TrEMBL

A0A178WLB2

Conserved Domains (5) summary

smart00667
Location:4 → 34

LisH; Lissencephaly type-1-like homology motif

smart00668
Location:34 → 92

CTLH; C-terminal to LisH motif

COG2319
Location:360 → 748

WD40; WD40 repeat [General function prediction only]

sd00039
Location:423 → 460

7WD40; WD40 repeat [structural motif]

cl02567
Location:359 → 667

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_101443.4 → NP_563981.1 Transducin family protein / WD-40 repeat family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_563981.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94AI7, Q9LMQ9

UniProtKB/TrEMBL

A0A178WLB2

Conserved Domains (5) summary

smart00667
Location:4 → 34

LisH; Lissencephaly type-1-like homology motif

smart00668
Location:34 → 92

CTLH; C-terminal to LisH motif

COG2319
Location:360 → 748

WD40; WD40 repeat [General function prediction only]

sd00039
Location:423 → 460

7WD40; WD40 repeat [structural motif]

cl02567
Location:359 → 667

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001035973.3 → NP_001031050.1 Transducin family protein / WD-40 repeat family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031050.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94AI7, Q9LMQ9

UniProtKB/TrEMBL

A0A178WLB2

Conserved Domains (5) summary

smart00667
Location:4 → 34

LisH; Lissencephaly type-1-like homology motif

smart00668
Location:34 → 92

CTLH; C-terminal to LisH motif

COG2319
Location:360 → 748

WD40; WD40 repeat [General function prediction only]

sd00039
Location:423 → 460

7WD40; WD40 repeat [structural motif]

cl02567
Location:359 → 667

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...