TLR4 toll like receptor 4 [Sus scrofa (pig)] - Gene

Summary

Official Symbol: TLR4provided by VGNC
Official Full Name: toll like receptor 4provided by VGNC
Primary source: VGNC:VGNC:94025
See related: Ensembl:ENSSSCG00000005503
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Sus scrofa
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus
Expression: Broad expression in spleen (RPKM 43.3), lung (RPKM 32.5) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 1

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
106	current	Sscrofa11.1 (GCF_000003025.6)	1	NC_010443.5 (258044610..258054641)
105	previous assembly	Sscrofa10.2 (GCF_000003025.5)	1	NC_010443.4 (289775816..289785847)

Chromosome 1 - NC_010443.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Transcriptome data used for gene annotation
Description: Nine kinds of tissues and a mixture of multiple tissues were sequenced for genome annotation
BioProject: PRJNA311523
Publication: PMID 27646534
Analysis date: Thu Dec 14 17:10:49 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55.
Title: High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55.
Data suggest that expression of TLR4 in intestinal mucosa can be regulated by dietary factors; here, flaxseed oil down-regulates expression of TLR4 in piglet model of necrotizing enterocolitis.
Title: Flaxseed Oil Attenuates Intestinal Damage and Inflammation by Regulating Necroptosis and TLR4/NOD Signaling Pathways Following Lipopolysaccharide Challenge in a Piglet Model.
Actinobacillus pleuropneumoniae induces alveolar Macrophages to produce proinflammatory cytokines via upregulation of TLR4 and NF-kappaB.
Title: Activation of Porcine Alveolar Macrophages by Actinobacillus pleuropneumoniae Lipopolysaccharide via the Toll-Like Receptor 4/NF-κB-Mediated Pathway.
The TWEAK-independent Fn14 activation augments TLR4-mediated inflammatory responses in the intestine of piglets.
Title: Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation.
These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets.
Title: Differential expression of Toll-like receptor 4 signaling pathway genes in Escherichia coli F18-resistant and - sensitive Meishan piglets.
Data suggest expression of TLR4 and NFKB (nuclear factor kappa B) are regulated by dietary factors affecting innate immunity; here, Lactobacillus acidophilus in feed down-regulates expression of TLR4 and NFKB in mononuclear cells after LPS challenge.
Title: Lactobacillus acidophilus modulates inflammatory activity by regulating the TLR4 and NF-κB expression in porcine peripheral blood mononuclear cells after lipopolysaccharide challenge.
At 30 days after autotransplantation of a pig kidney, mRNA expression increases for TLR4.
Title: Toll-like receptors 2 and 4 in pigs' kidneys early after autotransplantation procedure.
Data suggest TLR2, TLR4, and calcium signaling in enterocytes play principal roles in mucosal immunity against enterotoxigenic Escherichia coli; probiotic Lactobacillus delbrueckii and its extracellular polysaccharides appear to stimulate TLR2/TLR4.
Title: Lactobacillus delbrueckii TUA4408L and its extracellular polysaccharides attenuate enterotoxigenic Escherichia coli-induced inflammatory response in porcine intestinal epitheliocytes via Toll-like receptor-2 and 4.
TLR2 is required for the suppression of TLR4 signaling activation.
Title: Lactobacillus amylovorus inhibits the TLR4 inflammatory signaling triggered by enterotoxigenic Escherichia coli via modulation of the negative regulators and involvement of TLR2 in intestinal Caco-2 cells and pig explants.
The current study screened for single nucleotide polymorphisms (SNPs) in the TLR4 gene and tested their association with Salmonella fecal shedding.
Title: TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs.

Submit: New GeneRIF Correction See all GeneRIFs (23)

General gene information

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Gene Ontology Provided by RefSeq

Function	Evidence Code	Pubs
enables lipopolysaccharide binding	IEA Inferred from Electronic Annotation more info	PubMed
enables lipopolysaccharide immune receptor activity	IEA Inferred from Electronic Annotation more info	PubMed
enables protein binding	IEA Inferred from Electronic Annotation more info	PubMed
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info	PubMed

Process	Evidence Code	Pubs
involved_in defense response to Gram-negative bacterium	IEA Inferred from Electronic Annotation more info	PubMed
involved_in immune response	IEA Inferred from Electronic Annotation more info	PubMed
involved_in inflammatory response	IEA Inferred from Electronic Annotation more info	PubMed
involved_in toll-like receptor signaling pathway	IEA Inferred from Electronic Annotation more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info	PubMed

General protein information

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Preferred Names: toll-like receptor 4

NP_001106510.2

EC 3.2.2.6

NP_001280245.1

EC 3.2.2.6

NP_001280246.1

EC 3.2.2.6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113039.2 → NP_001106510.2 toll-like receptor 4 isoform 1 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AK396573

UniProtKB/TrEMBL

A0A480K9W0, A0A8D1GYP2, A0A8D1VNA8, C9EHS1, Q2TNK4

Related

ENSSSCP00000060828.1, ENSSSCT00000076294.2

Conserved Domains (4) summary

smart00255
Location:674 → 816

TIR; Toll - interleukin 1 - resistance

PLN00113
Location:47 → 532

PLN00113; leucine-rich repeat receptor-like protein kinase; Provisional

sd00031
Location:56 → 79

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:375 → 400

LRR_RI; leucine-rich repeat [structural motif]
NM_001293316.1 → NP_001280245.1 toll-like receptor 4 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AK396573, HM026368

UniProtKB/TrEMBL

A0A4X1UT03, A0A8D0QJW4, A0A8D1VP56, F1SMI0

Related

ENSSSCP00000005897.3, ENSSSCT00000006051.5

Conserved Domains (5) summary

smart00255
Location:432 → 574

TIR; Toll - interleukin 1 - resistance

cd00116
Location:128 → 340

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00031
Location:91 → 110

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:133 → 158

LRR_RI; leucine-rich repeat [structural motif]

pfam13855
Location:230 → 290

LRR_8; Leucine rich repeat
NM_001293317.1 → NP_001280246.1 toll-like receptor 4 isoform 3 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 3, which lacks a large internal in-frame segment and is shorter, compared to isoform 1.

Source sequence(s)

AK396573, DB797683

Conserved Domains (3) summary

sd00031
Location:56 → 79

LRR_1; leucine-rich repeat [structural motif]

pfam01582
Location:73 → 221

TIR; TIR domain

pfam13855
Location:55 → 87

LRR_8; Leucine rich repeat

RNA

NR_120680.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks an internal exon and uses an alternate splice site in the 3' terminal exon, compared to variant 1. This variant is represented as non-coding because the predicted protein does not meet RefSeq quality criteria.

Source sequence(s)

AK230475, AK396573