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MTF-1 Metal response element-binding Transcription Factor-1 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 39089, updated on 2-Nov-2024

Summary

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Official Symbol
MTF-1provided by FlyBase
Official Full Name
Metal response element-binding Transcription Factor-1provided by FlyBase
Primary source
FLYBASE:FBgn0040305
Locus tag
Dmel_CG3743
See related
AllianceGenome:FB:FBgn0040305
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
anon-WO0118547.411; CG3743; Dmel\CG3743; dMTF-1; mtf-1; Mtf-1
Summary
Enables DNA-binding transcription factor activity and transcription cis-regulatory region binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to copper ion; and stress response to metal ion. Located in cytoplasm and nucleus. Is expressed in several structures, including embryonic/larval garland cell; embryonic/larval midgut primordium; garland cell primordium; gut section; and larval muscle cell. Orthologous to human MTF1 (metal regulatory transcription factor 1). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs
all
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Genomic context

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See MTF-1 in Genome Data Viewer
Location:
67B5-67B6; 3-29 cM
Exon count:
7
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (9430312..9439490)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (9423412..9432590)

Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene SHC-adaptor protein Neighboring gene Ribosomal protein S17 Neighboring gene uncharacterized protein Neighboring gene antisense RNA:CR45872 Neighboring gene Blocked early in transport 3 Neighboring gene Nuclear factor Y-box A Neighboring gene Sting-regulated gene 3

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. An in-vivo microfluidic assay reveals cardiac toxicity of heavy metals and the protective effect of metal responsive transcription factor (MTF-1) in Drosophila model. Zabihihesari A, et al. 3 Biotech, 2022 Oct. PMID 36275358, Free PMC Article
  2. Expression and localisation of the essential copper transporter DmATP7 in Drosophila neuronal and intestinal tissues. Burke R, et al. Int J Biochem Cell Biol, 2008. PMID 18321764
  3. Gawky modulates MTF-1-mediated transcription activation and metal discrimination. Jia R, et al. Nucleic Acids Res, 2021 Jun 21. PMID 34107019, Free PMC Article
  4. Mercury and cadmium trigger expression of the copper importer Ctr1B, which enables Drosophila to thrive on heavy metal-loaded food. Balamurugan K, et al. Biol Chem, 2009 Feb. PMID 19040355
  5. Overexpression of metal-responsive transcription factor (MTF-1) in Drosophila melanogaster ameliorates life-span reductions associated with oxidative stress and metal toxicity. Bahadorani S, et al. Neurobiol Aging, 2010 Jul. PMID 18775584

See all (83) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Gawky modulates MTF-1-mediated transcription activation and metal discrimination.
    Title: Gawky modulates MTF-1-mediated transcription activation and metal discrimination.
  2. according to these results, a reduced expression of at least some of the zinc transporters is sufficient to normalize iron content. We also found that overexpression of MTF-1 improves the iron accumulation phenotype as well. In contrast, knocking down MtnA had no effect on the iron content
    Title: Metal Homeostasis Regulators Suppress FRDA Phenotypes in a Drosophila Model of the Disease.
  3. In addition, both metal specific domains are autorepressive in the absence of metal and contribute to the low level of basal transcription from metal inducible promoters.
    Title: Efficient metal-specific transcription activation by Drosophila MTF-1 requires conserved cysteine residues in the carboxy-terminal domain.
  4. Data indicate that MTF-1 is capable of binding to different regions of MtnA and Ctr1b genes depending on different metal stimulation.
    Title: Single nucleotide in the MTF-1 binding site can determine metal-specific transcription activation.
  5. Activation of this gene induced under opposite conditions of copper load and copper starvation manifested itself in a shortened lifespan, crippled wings, and female sterility.
    Title: Dissection of Drosophila MTF-1 reveals a domain for differential target gene activation upon copper overload vs. copper starvation.
  6. propose that Parkin and MTF-1 provide complementary functions in metal homeostasis, oxidative stress and other cellular stress response
    Title: The parkin mutant phenotype in the fly is largely rescued by metal-responsive transcription factor (MTF-1).
  7. When metal homeostasis is compromised by a mutation in metal-responsive transcription factor MTF-1, the life-span of the fly is shortened.
    Title: Overexpression of metal-responsive transcription factor (MTF-1) in Drosophila melanogaster ameliorates life-span reductions associated with oxidative stress and metal toxicity.
  8. Characterization of a novel cysteine-rich domain which is crucial for sensing excess intracellular copper by dMTF-1.
    Title: Copper sensing function of Drosophila metal-responsive transcription factor-1 is mediated by a tetranuclear Cu(I) cluster.
  9. The MTF-1's major role in both organisms is to control the transcription of genes involved in the homeostasis and detoxification of heavy metal ions such as Cu2+, Zn2+ and Cd2+.
    Title: Metal-responsive transcription factor (MTF-1) and heavy metal stress response in Drosophila and mammalian cells: a functional comparison.
  10. knockout of the protein in Drosophila by homologous recombination reveals its central role in heavy metal homeostasis
    Title: Knockout of 'metal-responsive transcription factor' MTF-1 in Drosophila by homologous recombination reveals its central role in heavy metal homeostasis.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables DNA-binding transcription factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transcription cis-regulatory region binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cellular response to oxidative stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in response to copper ion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to metal ion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in stress response to metal ion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
metal response element-binding transcription Factor-1
Names
CG3743-PA
CG3743-PB
CG3743-PC
CG3743-PD
CG3743-PE
CG3743-PF
CG3743-PG
MTF-1-PA
MTF-1-PB
MTF-1-PC
MTF-1-PD
MTF-1-PE
MTF-1-PF
MTF-1-PG
Metal-responsive transcription factor 1
metal response element binding transcription factor
metal response element binding transcription factor 1
metal response element-binding transcription factor-1
metal responsive transcription factor
metal responsive transcription factor 1
metal-regulatory transcription factor 1
metal-responsive transcription factor-1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_037436.4 Reference assembly

    Range
    9430312..9439490
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001300073.1 → NP_001287002.1  metal response element-binding transcription Factor-1, isoform F [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001287002.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    X2JCQ6
    Conserved Domains (5) summary
    COG5048
    Location:442 → 519
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:420 → 440
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:418 → 440
    zf-C2H2; Zinc finger, C2H2 type
    pfam13465
    Location:432 → 458
    zf-H2C2_2; Zinc-finger double domain
    pfam17017
    Location:329 → 471
    zf-C2H2_aberr; Aberrant zinc-finger

  2. NM_001104090.2 → NP_001097560.1  metal response element-binding transcription Factor-1, isoform C [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097560.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8JNP3
    Conserved Domains (6) summary
    COG5048
    Location:442 → 519
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:420 → 440
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:418 → 440
    zf-C2H2; Zinc finger, C2H2 type
    pfam12446
    Location:862 → 931
    DUF3682; Protein of unknown function (DUF3682)
    pfam13465
    Location:432 → 458
    zf-H2C2_2; Zinc-finger double domain
    pfam17017
    Location:329 → 471
    zf-C2H2_aberr; Aberrant zinc-finger

  3. NM_001104091.2 → NP_001097561.1  metal response element-binding transcription Factor-1, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097561.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q8IQC5, Q95TF7
    Conserved Domains (4) summary
    COG5048
    Location:6 → 83
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:12 → 34
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam12446
    Location:426 → 495
    DUF3682; Protein of unknown function (DUF3682)
    pfam13465
    Location:26 → 53
    zf-H2C2_2; Zinc-finger double domain

  4. NM_001300074.1 → NP_001287003.1  metal response element-binding transcription Factor-1, isoform G [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001287003.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q8IQC5, Q95TF7
    Conserved Domains (4) summary
    COG5048
    Location:6 → 83
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:12 → 34
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam12446
    Location:426 → 495
    DUF3682; Protein of unknown function (DUF3682)
    pfam13465
    Location:26 → 53
    zf-H2C2_2; Zinc-finger double domain

  5. NM_140054.3 → NP_648311.2  metal response element-binding transcription Factor-1, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_648311.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9GRX2, Q9VSY7
    Related
    FBpp0076187
    Conserved Domains (6) summary
    COG5048
    Location:227 → 304
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:205 → 225
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam00096
    Location:203 → 225
    zf-C2H2; Zinc finger, C2H2 type
    pfam12446
    Location:647 → 716
    DUF3682; Protein of unknown function (DUF3682)
    pfam13465
    Location:217 → 243
    zf-H2C2_2; Zinc-finger double domain
    pfam17017
    Location:114 → 256
    zf-C2H2_aberr; Aberrant zinc-finger

  6. NM_168343.2 → NP_729491.1  metal response element-binding transcription Factor-1, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_729491.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q8IQC5, Q95TF7
    Related
    FBpp0076188
    Conserved Domains (4) summary
    COG5048
    Location:6 → 83
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:12 → 34
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam12446
    Location:426 → 495
    DUF3682; Protein of unknown function (DUF3682)
    pfam13465
    Location:26 → 53
    zf-H2C2_2; Zinc-finger double domain

  7. NM_001104092.2 → NP_001097562.1  metal response element-binding transcription Factor-1, isoform E [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097562.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q8IQC5, Q95TF7
    Conserved Domains (4) summary
    COG5048
    Location:6 → 83
    COG5048; FOG: Zn-finger [General function prediction only]
    sd00017
    Location:12 → 34
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam12446
    Location:426 → 495
    DUF3682; Protein of unknown function (DUF3682)
    pfam13465
    Location:26 → 53
    zf-H2C2_2; Zinc-finger double domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A8JNP3 A8JNP3
Q8IQC5 Q8IQC5
Q95TF7 Q95TF7
Q9GRX2 Q9GRX2
Q9NFS1 Q9NFS1
Q9VSY7 Q9VSY7
X2JCQ6 X2JCQ6

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