GEO DataSets

(Submitter supplied) We used ribosome profiling (Ribo-seq) to generate a translatome map for the archaeon M. mazei under two growth conditions. The analysis of the datasets allowed to generate a high confidence inventory of small proteins in the model archaeon M. mazei and their regulation under nitrogen limiting conditions.

Organism:

Methanosarcina mazei Go1

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL33665

8 Samples

Download data: BW

(Submitter supplied) CRISPR loci are found in bacterial and archaeal genomes where they provide the molecular machinery for acquisition of immunity against foreign DNA. In addition to the cas genes fundamentally required for CRISPR activity, a second class of genes is associated with the CRISPR loci, of which many have no reported function in CRISPR-mediated immunity. Here, we characterize MM_0565 of Methanosarcina mazei Gö1 associated to the type I-B CRISPR-locus providing evidence for its relevance in regulating this system. more...

Organism:

Methanosarcina mazei Go1

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28591

4 Samples

Download data: TSV, WIG

(Submitter supplied) Trans-encoded sRNA154 is exclusively expressed under nitrogen (N)-deficiency in Methanosarcina mazei strain Gö1. The respective deletion strain showed a significant growth defect under N-limitation, pointing towards a regulatory role of sRNA154 in the N-metabolism. Aiming to elucidate this regulatory function we characterized sRNA154 by biochemical and genetic approaches. 24 homologs of sRNA154 were identified in recently reported draft genomes of Methanosarcina strains, demonstrating high conservation in sequence and predicted secondary structure with two highly conserved single stranded loop regions. more...

Organism:

Methanosarcina mazei Go1

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22308

4 Samples

Download data: WIG