GEO DataSets

Expression profiling of embryonic fibroblasts deleted for the transcription factor CHOP. Fibroblast treated with 2 ug/ml tunicamycin for 4 or 8 hours to induce endoplasmic reticulum (ER) stress. Results provide insight into molecular mechanisms regulating the response to ER stress.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation, 3 time sets

Platform:

GPL81

Series:

GSE2082

24 Samples

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(Submitter supplied) Primart mouse embryonic fibroblasts (pass 3 to 4) were treated with tunicamycin (2 micrograms/ml) for specified times - genotypes were WILDTYPE and CHOP-/-. Each time point n=4 Keywords: ordered

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS997

Platform:

GPL81

24 Samples

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(Submitter supplied) Accumulation of misfolded proteins in the endoplasmic reticulum (ER) triggers the unfolded protein response (UPR), which results in the increased phosphorylation of the eukaryotic initiation factor, eIF2a, and widespread translational repression. Protein synthesis is subsequently restored following the stress-induced transcriptional upregulation of GADD34 (growth arrest and DNA damage transcript 34) protein, a regulator of an eIF2a phosphatase. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL17021

40 Samples

Download data: XLSX

(Submitter supplied) The unfolded protein response (UPR) couples cellular translation rates and gene expression to the protein folding status of the endoplasmic reticulum (ER). Upon activation, the UPR machinery elicits a general suppression of protein synthesis and activation of stress gene expression, which act coordinately to restore protein folding homeostasis. We report here that UPR activation promotes the release of signal sequence-encoding mRNAs from the ER to the cytosol as a mechanism to decrease protein influx into the ER. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL13112

52 Samples

Download data: XLSX

(Submitter supplied) Purpose: The purpose of this data is to compare the transcriptome of embryonic stem cell (ESCs), Induced pluripotent stem cell (iPSCs) and fibroblasts using RNA-seq. Methods: Total RNA was harvested by Trizol (Invitrogen) and RNA profiles of samples were generated by sequencing. Illumina Casava 1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence (using fastx_trimmer) then mapped to hg19 (genome build) whole genome using Tophat. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

3 Samples

Download data: TXT