GEO DataSets

Expression profiling of cultured bladder smooth muscle cells subjected to repetitive mechanical stimulation for 4 hours. Chronic overdistension results in bladder wall thickening, associated with loss of muscle contractility. Results identify genes whose expression is altered by mechanical stimuli.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL96

Series:

GSE1595

8 Samples

Download data

(Submitter supplied) The aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS906

Platform:

GPL96

8 Samples

Download data

(Submitter supplied) Tissue Factor Pathway Inhibitor-2 is Induced by Fluid Shear Stress in Vascular Smooth Muscle Cells and Affects Cell Proliferation and Survival Introduction: Vascular smooth muscle cells (SMCs) are exposed to fluid shear stress (FSS) after interventional procedures such as balloon-angioplasty. Whereas the effects of hemodynamic forces on endothelial cells are explored in detail, the influence of FSS on smooth muscle cell function is poorly characterized. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

6 Samples

Download data: CEL

(Submitter supplied) To explore global molecular changes in smooth muscle in response to PDGFR activation, primary human bladder smooth muscle cells were treated with 1 nM PDGF-BB (hereafter PDGF) for 0, 4 or 24 h. Total RNA were prepared, and analyzed using expression profiling, and subjected to bioinformatic and functional interrogation.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

12 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rattus norvegicus; synthetic construct

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL21572 GPL17117

24 Samples

Download data: CEL, CHP

(Submitter supplied) Bilateral freezing of the pelvic ganglia in female rats were performed to denervate the urinary bladder. Sham operated rats were used as controls. The rats were sacrificed 10 days after surgery. The urinary bladders (including the urothelium) were frozen and used for RNA extraction.

Organism:

Rattus norvegicus; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL21572

12 Samples

Download data: CEL, CHP

(Submitter supplied) Bilateral freezing of the pelvic ganglia in female rats were performed to denervate the urinary bladder. Sham operated rats were used as controls. The rats were sacrificed 10 days after surgery. The urinary bladders (including the urothelium) were frozen and used for RNA extraction.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

12 Samples

Download data: CEL, CHP

(Submitter supplied) Using whole-genome Affymetrix microarrays (HG-U133A), we characterized the transcriptome profile of cultured human macrophages stimulated for 4 h with interleukin 1 (IL-1) or interleukin 6 (IL-6). We found that, in distinction to liver cells, IL-1 is much more potent than IL-6 in modifying macrophage gene expression, although considerable heterogeneity in response of macrophages deriving from individual blood donors was observed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3005

Platform:

GPL96

15 Samples

Download data: CEL

Analysis of cultured monocyte-derived macrophages (MDM) stimulated with interleukin (IL) 1 or IL-6 for 4 hours. Cytokines IL-1 and IL-6 are not only produced by macrophages but also affect the cells in an autocrine manner. Results provide insight into IL-1 and IL-6 responsive genes in MDMs.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent sets

Platform:

GPL96

Series:

GSE8515

15 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1779

Platform:

GPL570

12 Samples

Download data

(Submitter supplied) Oxygen is vital for the development and survival of mammals. In response to hypoxia, the brain initiates numerous adaptive responses at the organ level as well as at the molecular and cellular level, including the alteration of gene expression. Astrocytes play critical roles in the proper functioning of the brain, thus the manner in which astrocytes respond to hypoxia is likely important in determining the outcome of brain hypoxia. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data

(Submitter supplied) Oxygen is vital for the development and survival of mammals. In response to hypoxia, the brain initiates numerous adaptive responses at the organ level as well as at the molecular and cellular level, including the alteration of gene expression. Astrocytes play critical roles in the proper functioning of the brain, thus the manner in which astrocytes respond to hypoxia is likely important in determining the outcome of brain hypoxia. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data

Analysis of purified primary astrocytes exposed to hypoxia for 24 hours. Gene expression in HeLa cells also examined. Astrocytes play critical roles in the proper functioning of the brain. Results provide insight into the molecular events underlying the astrocytic responses to hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type, 2 stress sets

Platform:

GPL570

Series:

GSE4483

12 Samples

Download data

(Submitter supplied) The Eol1 cell line has been derived from a patient with chronic eosiniphilic leukemia. Eol1 cells express the FIP1L1-PDGFRalpha oncogene. Inhibition of FIP1L1-PDGFRalpha with imatinib mesylate (Glivec) blocks proliferation and survival of the cells. We performed microarray expression analysis to identify genes specifically regulated by FIP1L1-PDGFRalpha using imatinib-treated cells as baseline. The list of regulated genes was consistent with the activation of STAT trancription factors by FIP1L1-PDGFRA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL, CHP

(Submitter supplied) We analyzed gene expression in human fibroblasts stimulated by platelet-derived growth factor-BB (PDGF-BB) or basic fibroblast growth factor (bFGF) for 1h and 24h. The results of two independent experiments were merged. SAM analysis identified 116 relevant probe sets. Hierarchical clustering of these probe sets showed divergent early gene regulation by PDGF and FGF but overlapping late response. We first analyzed genes commonly regulated by PDGF-BB and b-FGF more than 2 fold after 24h of stimulation and we found that these two growth factors repressed FOXO. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

10 Samples

Download data: CEL, CHP

(Submitter supplied) The effect of cyclic mecanical stretch on cardiac gene expression was studied in neonatal rat ventricular myocytes (NRVMs).

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

35 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; Mus musculus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human immunodeficiency virus 1; Rattus norvegicus; Murid gammaherpesvirus 4; Betapolyomavirus hominis; human gammaherpesvirus 4; JC polyomavirus; Human gammaherpesvirus 8; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array

Platform:

GPL7722

20 Samples

Download data

(Submitter supplied) The effect of cyclic mecanical stretch on cardiac microRNA expression was studied in neonatal rat ventricular myocytes (NRVMs).

Organism:

Homo sapiens; Mus musculus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; human gammaherpesvirus 4; Betapolyomavirus macacae; Rattus norvegicus; JC polyomavirus; Human gammaherpesvirus 8; Betapolyomavirus hominis

Type:

Non-coding RNA profiling by array

Platform:

GPL7722

9 Samples

Download data: XLS

(Submitter supplied) The effect of cyclic mecanical stretch on cardiac microRNA expression was studied in neonatal rat ventricular myocytes (NRVMs).

Organism:

Homo sapiens; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human immunodeficiency virus 1; Human alphaherpesvirus 1; human gammaherpesvirus 4; Betapolyomavirus macacae; Mus musculus; Human gammaherpesvirus 8; Rattus norvegicus; JC polyomavirus; Murid gammaherpesvirus 4; Betapolyomavirus hominis

Type:

Non-coding RNA profiling by array

Platform:

GPL7722

11 Samples

Download data: XLS

(Submitter supplied) In vitro time course experiment using the A404 clone of P19 mouse embryonal carcinoma cells to evaluate SMC differentiation in response to retinoic acid followed by phenotype-specific selection with puromycin. Four time points of 5-6 biological replicates each were performed. Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS799

Platform:

GPL1307

23 Samples

Download data