GEO DataSets

Temporal transcriptional program of primary fibroblasts in response to serum. Samples taken at various time points up to 24 hours.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 10 time sets

Platform:

GPL169

Series:

GSE58

10 Samples

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(Submitter supplied) The temporal program of gene expression during a model physiological response of human cells, the response of fibroblasts to serum, was explored with a complementary DNA microarray representing about 8600 different human genes. Genes could be clustered in groups on the basis of their temporal patterns of expression in this program. Many features of the transcriptional program appeared to be related to the physiology of wound repair suggesting that fibroblasts play a larger and richer role in this complex multicellular response than had previously been appreciated. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS85 GDS86

Platforms:

GPL168 GPL169

18 Samples

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(Submitter supplied) The transcriptional program in the response of human fibroblasts to serum control group. This study is described more fully in Iyer VR, et al. 1999. Science 283:83-7 Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL169

2 Samples

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(Submitter supplied) The transcriptional program in the response of human fibroblasts to serum. Serum was added to fibroblasts in the presence of cycloheximide. This study is described more fully in Iyer VR, et al. 1999. Science 283:83-7 Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS145

Platform:

GPL169

4 Samples

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(Submitter supplied) The transcriptional program in the response of human fibroblasts to serum. Serum was added to fibroblasts, and samples were taken. This study is described more fully in Iyer VR, et al. 1999. Science 283:83-7 Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL168 GPL169

12 Samples

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Analysis of the transcriptional program in the response of human fibroblasts to serum. Serum added to fibroblasts in the presence of cycloheximide and samples taken at 0, 0.5, 2 and 4 hours.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 4 time sets

Platform:

GPL169

Series:

GSE56

4 Samples

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Analysis of a temporal transcriptional program in the response of primary fibroblast cultures to serum. Samples taken at 15 minutes and 2 hours.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 2 time sets

Platform:

GPL168

Series:

GSE58

2 Samples

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(Submitter supplied) PI-3K inhibitor (LY294002) was added to quiescent fibroblasts 30 minutes prior growth factor/serum treatments. The role of PI-3K pathway was thus monitored by comparing with untreated samples. Keywords: time course, growth factor response, cell line comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3279

20 Samples

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(Submitter supplied) Quiescent human fibroblasts (2091 and Wi-38) were stimulated with different growth factors and serum. Cells were collected at 6 different time points followed by global transcriptional profiling. Keywords: time course, growth factor response, cell line comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3279

120 Samples

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(Submitter supplied) The skin is a protective barrier against external insults and any lesion must be rapidly and efficiently repaired. Dermal fibroblasts are the major source of extracellular connective tissue matrix and play an important role in wound healing. Vitamin C is an important water-soluble free radical scavenger and an essential cofactor for collagen synthesis by dermal fibroblasts and, consequently, may contribute to the maintenance of healthy skin. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3635

Platform:

GPL570

9 Samples

Download data: CEL

Analysis of GM5659 skin fibroblasts treated with ascorbic acid or the stable vitamin C derivative, ascorbic acid 2-phosphate. Vitamin C is an important water-soluble free radical scavenger and an essential cofactor for collagen synthesis by dermal fibroblasts.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent sets

Platform:

GPL570

Series:

GSE11919

9 Samples

Download data: CEL

(Submitter supplied) Human cytomegalovirus (HCMV) has been shown to have the potential to alter cellular gene expression early after infection. However, one-gene approaches and the use of closed system gene expression technologies have identified only few cellular genes whose activity changed immediate-early. We therefore used serial analysis of gene expression (SAGE) to investigate the transcriptional program of human fibroblasts in response to HCMV in the immediate-early phase of infection. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

2 Samples

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(Submitter supplied) Wound healing within the oral mucosa results in minimal scar formation compared to wounds within the skin. We have recently demonstrated distinct differences in the ageing profiles of cells (oral mucosal and patient-matched skin fibroblasts) isolated from these tissues. We hypothesize that the increased replicative potential of oral mucosal fibroblasts may confer upon them preferential wound healing capacities. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3856

Platform:

GPL96

15 Samples

Download data: CEL, CHP

Temporal analysis of oral mucosal (OM) fibroblasts and patient-matched skin fibroblasts following serum starvation/induction. OM fibroblasts exhibit a preferential wound-healing response compared to that of dermis. Results provide insight into molecular basis of OM fibroblast wound healing response.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type, 4 individual, 2 protocol, 2 time sets

Platform:

GPL96

Series:

GSE21648

15 Samples

Download data: CEL, CHP

(Submitter supplied) Rationale: During excessive pressure or overload, cardiac cells are subjected to increased mechanical stress. We investigate how the stress response of cardiac cells to mechanical stress can be compared to genotoxic stresses induced by DNA damaging agents. Methods: Cultures enriched for cardiomyocytes and cultures of fibroblasts were derived from ventricles of neonatal rat hearts. In three independent experiments, both cell cultures were subjected to mechanical stress (cyclic stretch at 60 cycles/min), UV irradiation (10 J/m2), or X-rays (8.5 Gy). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL85

35 Samples

Download data: CEL, EXP

(Submitter supplied) Maggot ES is known to induce wound healing in vivo to improve chronic wound repair. The effects have been studies at the protein and molecular level but never before at the transcriptional level. Affymetrix microarrays were used to identify the genes modulated by maggot ES and link these to gene pathways to understand the molecular effects on cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL15207 GPL6244

24 Samples

Download data: CEL, XLSX

(Submitter supplied) In mammals, increasing evidence supports the generality of cotranscriptional gene regulation and the idea that genetic control often occurs subsequent to RNA polymerase II (Pol II) recruitment. In our recent experiments, we have used Pol II Chromatin Immunoprecipitation (ChIP) to investigate relationships between the mechanistic events that control acute stress response (ASR) gene activation following stimulation of the alpha1a-Adrenergic Receptor expressed in rat-1 fibroblasts. more...

Organism:

Rattus norvegicus; Rattus rattus

Type:

Expression profiling by array

Platform:

GPL9207

20 Samples

Download data: GPR

(Submitter supplied) To elucidate biological processes underlying the keratocyte, fibroblast, and myofibroblast phenotypes of corneal stromal cells, the gene expression patterns of these primary cultures from mouse cornea were compared with those of the adult and 10-day postnatal mouse cornea. Keywords = corneal stroma maturation keratocyte fibroblast myofibroblast TGF beta 1 Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS857

Platform:

GPL81

14 Samples

Download data: CEL

Expression profiling of cultured keratocyte, fibroblast, and myofibroblast cells of the corneal stroma. Cultured cells compared to cornea from adults and 10 day postnatals. Keratocytes maintain the transparent corneal stroma, and upon injury differentiate into fibroblasts and myofibroblasts.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 cell type, 3 protocol sets

Platform:

GPL81

Series:

GSE1867

14 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

5 related Platforms

34 Samples

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