GEO DataSets

Analysis of highly glycolytic A549 lung cancer cells depleted for steroid receptor coactivator SRC-1 and grown in the absence of glucose. SRC-1 promotes gluconeogenesis and glycemia. Results provide insight into the role of SRC-1 in glycolytic cancer cells undergoing glucose deprivation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL570

Series:

GSE56843

8 Samples

Download data: CEL

(Submitter supplied) SRC-1 affects the expression of complex I of the mitochondrial electron transport chain, a set of enzymes responsible for the conversion of NADH to NAD(+). NAD(+) and NADH were subsequently identified as metabolites that underlie SRC-1's response to glucose deprivation. Knockdown of SRC-1 in glycolytic cancer cells abrogated their ability to grow in the absence of glucose consistent with SRC-1's role in promoting cellular adaptation to reduced glucose availability

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5418

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) The molecular targets of SRC-2 regulation in the murine liver stimulate fatty acid degradation and glycolytic pathway while fatty acid, cholesterol, and steroid biosynthetic pathways are down-regulated.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4785

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of livers from C57 females lacking Steroid receptor coactivator-2 (SRC-2). SRC-2 is a mediator of steroid receptor action. Results provide insight into the role of steroid receptor coactivators in hepatic metabolism.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE55002

6 Samples

Download data: CEL

(Submitter supplied) In this study we explore the genomic re-wiring of a human colon cancer cell line (LS174T) in which fermentative glycolysis has been fully suppressed by CRISPR/Cas9 to disrupt glucose-6-phosphate isomerase (GPI-KO) or dual disruption of lactate dehydrogenases A and B (LDHAB-DKO). The effects of both KOs, had no effect on loss of viable cell function, corresponding to high concentrations of ATP production vs WT. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

19 Samples

Download data: CEL, CHP

(Submitter supplied) SRC-1 (NCOA1) is a steroid receptor coactivator that has been associated with various aspects of the progression of breast cancer disease such as tamoxifen resistance, metastasis, cell proliferation and invasiveness. In a tamoxifen resistant breast cancer cell line (LY2), SRC-1 has been found to interact with the developmental transcription factor HoxC11. ChIP-sequencing of HoxC11 in LY2 cells shows where the transcription factor binds throughout the genome.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9115

3 Samples

Download data: BIGWIG

(Submitter supplied) We profile the binding of Steroid Receptor Co-activator (SRC1) in LY2 cells, a tamoxifen-resistant cell line, in the presence and absence of tamoxifen using ChIP-sequencing technology. The development of breast cancer resistance to endocrine therapy results from an increase in cellular plasticity leading to the development of a steroid-independent tumour. The p160 steroid coactivataor protein SRC-1, through interactions with developmental proteins and other non-steroidal transcription factors, drives this tumour adaptability. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9115

3 Samples

Download data: MAP, WIG

(Submitter supplied) The development of breast cancer resistance to endocrine therapy results from an increase in cellular plasticity leading to the development of a steroid independent tumour. The p160 steroid coactivator protein SRC-1, through interactions with developmental proteins and other non-steroidal transcription factors drives this tumour adaptability. Here, using discovery studies we identify ADAM22, a non-protease member of the ADAMs family, as a direct target of SRC-1, independent of estrogen receptor(ER). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4095

Platform:

GPL570

14 Samples

Download data: CEL

Analysis of endocrine-resistant LY2 breast cancer cells depleted of p160 steroid coactivator protein SRC-1 and treated with tamoxifen. SRC-1 is central to the development of the endocrine resistant phenotype. Results provide insight into the molecular basis of endocrine resistant breast cancer.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 genotype/variation sets

Platform:

GPL570

Series:

GSE28645

14 Samples

Download data: CEL

(Submitter supplied) These arrays contain data from the livers of 10 week old L-Pex5 -/- male mice

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL